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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2171 [new locus tag: NWMN_RS12550 ]
  • pan locus tag?: SAUPAN005732000
  • symbol: moaE
  • pan gene symbol?: moaE
  • synonym:
  • product: molybdopterin synthase large subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2171 [new locus tag: NWMN_RS12550 ]
  • symbol: moaE
  • product: molybdopterin synthase large subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 2395659..2396105
  • length: 447
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAACAATTTGAAATCGTGACAGAACCGATACAAACAGAACAATATCGTGAATTCACT
    ATAAATGAATATCAAGGTGCAGTAGTTGTTTTTACCGGTCATGTTCGCGAATGGACTAAA
    GGCGTCAAAACGGAATATTTAGAATATGAAGCGTATATTCCAATGGCTGAAAAGAAATTG
    GCACAAATTGGAGATGAAATAAATGAAAAATGGCCTGGAACGATAACGAGTATTGTTCAT
    AGAATAGGGCCATTACAAATTTCAGATATCGCTGTATTAATTGCGGTTTCTTCACCGCAT
    CGTAAAGATGCCTATCGAGCAAATGAATATGCAATTGAGCGTATAAAAGAAATTGTTCCG
    ATTTGGAAAAAAGAAATTTGGGAAGATGGTTCAAAATGGCAAGGGCATCAAAAAGGGAAT
    TATGAAGAAGCGAAGAGGGAGGAATAA
    60
    120
    180
    240
    300
    360
    420
    447

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2171 [new locus tag: NWMN_RS12550 ]
  • symbol: MoaE
  • description: molybdopterin synthase large subunit
  • length: 148
  • theoretical pI: 5.35216
  • theoretical MW: 17338.5
  • GRAVY: -0.664189

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Molybdopterin synthase catalytic subunit MoaE (EC 2.8.1.12)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdenum cofactor biosynthesis protein MoaE
  • ⊞PFAM:
    no clan defined MoaE; MoaE protein (PF02391; HMM-score: 125.3)
    and 1 more
    Thioredoxin (CL0172) AhpC-TSA; AhpC/TSA family (PF00578; HMM-score: 15.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9994
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0005
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002814
    • TAT(Tat/SPI): 0.00042
    • LIPO(Sec/SPII): 0.000317
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRKDAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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