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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0699 [new locus tag: NWMN_RS03955 ]
  • pan locus tag?: SAUPAN002629000
  • symbol: nrdI
  • pan gene symbol?: nrdI
  • synonym:
  • product: ribonucleotide reductase stimulatory protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0699 [new locus tag: NWMN_RS03955 ]
  • symbol: nrdI
  • product: ribonucleotide reductase stimulatory protein
  • replicon: chromosome
  • strand: +
  • coordinates: 784263..784661
  • length: 399
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 5330403 NCBI
  • RefSeq: YP_001331733 NCBI
  • BioCyc:
  • MicrobesOnline: 3706246 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
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    ATGAAAATAATATATTTTTCATTTACTGGAAATGTCCGTCGTTTTATTAAGAGAACAGAA
    CTTGAAAATACGCTTGAGATTACAGCAGAAAATTGTATGGAACCAGTTCATGAACCGTTT
    ATTATCGTTACTGGCACTATTGGATTTGGAGAAGTACCAGAACCCGTTCAATCTTTTTTA
    GAAGTTAATCATCAATACATCAGAGGTGTGGCAGCTAGCGGTAATCGAAATTGGGGACTA
    AATTTCGCAAAAGCGGGTCGCACGATATCAGAAGAGTATAATGTCCCTTTATTAATGAAG
    TTTGAGTTACATGGAAAAAACAAAGACGTTATTGAATTTAAGAACAAGGTGGGTAATTTT
    AATGAAAACCATGGAAGAGAAAAAGTACAATCATATTGA
    60
    120
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    399

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0699 [new locus tag: NWMN_RS03955 ]
  • symbol: NrdI
  • description: ribonucleotide reductase stimulatory protein
  • length: 132
  • theoretical pI: 7.67183
  • theoretical MW: 15166.2
  • GRAVY: -0.422727

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism nrdI protein (TIGR00333; HMM-score: 115.3)
  • TheSEED  :
    • Ribonucleotide reduction protein NrdI
    Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Ribonucleotide reduction  Ribonucleotide reduction protein NrdI
  • PFAM:
    Flavoprotein (CL0042) Flavodoxin_NdrI; NrdI Flavodoxin like (PF07972; HMM-score: 126.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9903
    • Cytoplasmic Membrane Score: 0.005
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0046
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005252
    • TAT(Tat/SPI): 0.000118
    • LIPO(Sec/SPII): 0.001041
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 151220911 NCBI
  • RefSeq: YP_001331733 NCBI
  • UniProt: A6QF39 UniProt

⊟Protein sequence[edit | edit source]

  • MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVTGTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFELHGKNKDVIEFKNKVGNFNENHGREKVQSY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: nrdI > nrdE > nrdF

⊟Regulation[edit | edit source]

  • ⊞regulator: NrdR (repression) regulon
    NrdR(TF)important in Deoxyribonucleotide biosynthesis; RegPrecise 

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_0699&oldid=56953"
  • This page was last edited on 10 March 2016, at 13:28.
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