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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS01800 [old locus tag: SA0312 ]
  • pan locus tag?: SAUPAN001855000
  • symbol: SA_RS01800
  • pan gene symbol?: —
  • synonym:
  • product: LLM class flavin-dependent oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS01800 [old locus tag: SA0312 ]
  • symbol: SA_RS01800
  • product: LLM class flavin-dependent oxidoreductase
  • replicon: chromosome
  • strand: +
  • coordinates: 370176..371177
  • length: 1002
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (370176..371177) NCBI
  • BioCyc: SA_RS01800 BioCyc
  • MicrobesOnline: see SA0312

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGGTTAAATTAAGCGTATTAGACTATGCCTTAATAGATGAAGGTAAGGATGCACAAAAG
    GCATTGCAAGATTCAGTGACACTTGCAAAATTAGCAGATCGACTTGGCTTTAAGCGAATT
    TGGTTTACGGAACATCATAATGTACCAGCGTTTGCGTGTAGTAGTCCAGAACTTTTGATG
    ATGCATACATTGGCGCAGACAAATCACATACGAGTTGGCTCTGGTGGTGTGATGCTGCCG
    CACTATCGACCTTATAAAATTGCTGAGCATTTTAGAATGATGGCAGCGTTATATCCAAAT
    CGTATTGATTTAGGTATTGGTAATAATCCAGGTACTACTATGGTAAAGCAAGCTTTAGAT
    GGAATAAATCCTACATATGATAGTTACGATGAATCGATTTCGTTATTACGTGATTATCTT
    ACAATAAAGGATAAACCAAGTGCGCATACGTTAGGTGTCCAACCACACATTGATCATTTT
    CCAGAAATGTGGTTATTAAGTAGTAGCGCAACATCTGCCAAAATAGCTGCCGAACTAGGT
    ATAGGGCTTTCTGTTGGAACATTTTTGCTACCAGATATAAATGCGATACATGCAGCGAAG
    GATAACATTGATATTTACAAAAAACATTTCCAAGCATCAACGATTAAAATGGACGCAAAG
    GTGATGGCATCTGTATTTGTCATTGTAGCAGATAACGAAGCGGAAGTAGCAGCATTGCAA
    CATGCCTTAGATGTTTGGTTATTAGGTAAATTACAATTTGCAGAATTTGAACATTTTCCT
    TCAGTAGACACAGCACAAAAGTATAAGCTTAATGATCGAGACAAAGAGATGATTCAAGTA
    CATCAAGCACGCATCATTGCAGGCACACAAGAACAGGTTAAAGCACAATTAGATGATTTC
    ATTGCTACGTTTGAAGTTGATGAGGTGTTAGTAGCACCGCTTATTCCAGGTATTGAACAG
    CGTTGTAAAACATTAAAATTACTCGCGGAAATTTATTTGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS01800 [old locus tag: SA0312 ]
  • symbol: SA_RS01800
  • description: LLM class flavin-dependent oxidoreductase
  • length: 333
  • theoretical pI: 6.27138
  • theoretical MW: 37119.6
  • GRAVY: -0.0225225

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Unknown function Enzymes of unknown specificity luciferase family oxidoreductase, group 1 (TIGR03558; EC 1.-.-.-; HMM-score: 349)
    and 20 more
    Unknown function Enzymes of unknown specificity putative FMN-dependent luciferase-like monooxygenase, KPN_01858 family (TIGR04027; EC 1.-.-.-; HMM-score: 48.7)
    natural product biosynthesis luciferase-like monooxygenase domain (TIGR04020; EC 1.-.-.-; HMM-score: 42.8)
    Unknown function Enzymes of unknown specificity putative luciferase-like monooxygenase, FMN-dependent, CE1758 family (TIGR04036; EC 1.-.-.-; HMM-score: 41.3)
    probable oxidoreductase, LLM family (TIGR03858; EC 1.-.-.-; HMM-score: 38.3)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_3544 family (TIGR03854; HMM-score: 34.8)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv3093c family (TIGR03841; EC 1.-.-.-; HMM-score: 34.5)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_4141 family (TIGR03620; HMM-score: 28.6)
    TAT-translocated F420-dependent dehydrogenase, FGD2 family (TIGR04465; EC 1.-.-.-; HMM-score: 26.8)
    Unknown function Enzymes of unknown specificity F420-dependent oxidoreductase, MSMEG_4879 family (TIGR03564; EC 1.-.-.-; HMM-score: 26.1)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv2161c family (TIGR03619; EC 1.-.-.-; HMM-score: 25.1)
    Metabolism Energy metabolism Methanogenesis 5,10-methylenetetrahydromethanopterin reductase (TIGR03555; EC 1.5.98.2; HMM-score: 24.3)
    Metabolism Energy metabolism Pentose phosphate pathway glucose-6-phosphate dehydrogenase (coenzyme-F420) (TIGR03554; EC 1.1.98.2; HMM-score: 23.2)
    Unknown function Enzymes of unknown specificity luciferase-type oxidoreductase, BA3436 family (TIGR03571; EC 1.-.-.-; HMM-score: 22.2)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_2249 family (TIGR03857; HMM-score: 20.8)
    Unknown function Enzymes of unknown specificity F420-dependent oxidoreductase, CPS_4043 family (TIGR03842; HMM-score: 18.1)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_2516 family (TIGR03621; HMM-score: 16.8)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv3520c family (TIGR03559; HMM-score: 14.5)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv1855c family (TIGR03560; HMM-score: 14)
    Unknown function Enzymes of unknown specificity coenzyme F420-dependent oxidoreductase, NP1902A family (TIGR04024; EC 1.14.-.-; HMM-score: 12.8)
    Unknown function Enzymes of unknown specificity FMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family (TIGR03860; EC 1.-.-.-; HMM-score: 12.6)
  • TheSEED: see SA0312
  • PFAM:
    TIM_barrel (CL0036) Bac_luciferase; Luciferase-like monooxygenase (PF00296; HMM-score: 121.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.975
    • Cytoplasmic Membrane Score: 0.0067
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0179
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004806
    • TAT(Tat/SPI): 0.000337
    • LIPO(Sec/SPII): 0.000547
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 446162208 NCBI
  • RefSeq: WP_000240063 NCBI
  • UniProt: see SA0312

⊟Protein sequence[edit | edit source]

  • MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTEHHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRIDLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHFPEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMDAKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKEMIQVHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS01800&oldid=89325"
  • This page was last edited on 11 March 2016, at 07:20.
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