PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

organism: Staphylococcus aureus NCTC8325

locus tag: SAOUHSC_00973

pan locus tag^?: SAUPAN003234000

symbol: SAOUHSC_00973

pan gene symbol^?: tarM

synonym:
product:

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SAOUHSC_00973
symbol: SAOUHSC_00973
product:
replicon: chromosome
strand: +
coordinates: 947205..948687
length: 1482
essential: no DEG
comment: The sequence of SAOUHSC_00973 was corrected based on the resequencing performed by Berscheid et al., 2012 ^[1].

⊟Accession numbers[edit | edit source]

Gene ID: 3920118 NCBI
RefSeq:
BioCyc: G1I0R-917 BioCyc
MicrobesOnline: 1289439 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
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541
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661
721
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841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAAAAAAATATTTATGATGGTACATGAGTTAGATGTGAATAAAGGTGGTATGACCTCT
TCGATGTTCAATAGAAGTAAAGAGTTTTATGATGCGGACATACCTGCTGATATTGTTACT
TTCGATTACAAAGGAAACTATGATGAAATTATTAAAGCTTTGAAAAAACAAGGTAAAATG
GATCGAAGAACGAAAATGTATAATGTATTTGAGTATTTTAAACAAATTTCAAATAATAAA
CATTTTAAGTCTAATAAATTGTTATATAAACATATTTCAGAAAGACTAAAAAATACGATT
GAAATTGAAGAGAGTAAAGGTATTTCAAGATATTTTGATATAACGACTGGTACATATATT
GCCTACATTAGAAAAAGTAAATCTGAAAAAGTGATTGATTTCTTTAAAGATAATAAACGA
ATTGAACGGTTTAGTTTTATAGATAATAAAGTGCATATGAAGGAAACATTTAATGTAGAT
AATAAAGTTTGTTATCAAGTATTTTATGATGAAAAGGGATACCCATATATTTCAAGGAAT
ATTAATGCTAATAATGGTGCTGTAGGTAAAACTTATGTGTTAGTTAATAAAAAAGAATTT
AAAAACAATTTAGCACTGTGTGTTTACTATTTAGAAAAACTAATAAAAGATTCTAAAGAT
AGTATTATGATTTGTGATGGACCAGGGAGTTTTCCAAAAATGTTTAATACAAATCATAAA
AATGCTCAGAAATATGGCGTTATTCATGTTAATCATCATGAAAATTTCGATGATACGGGT
GCATTTAAAAAAAGTGAGAAATATATTATTGAGAATGCGAATAAAATTAACGGTGTAATT
GTATTAACAGAGGCACAAAGATTAGATATTCTTAATCAATTTGATGTAGAAAATATTTTC
ACTATTAGCAATTTTGTTAAGATACATAATGCTCCAAAACATTTTCAAACTGAAAAAATC
GTAGGTCATATTTCTAGAATGGTACCAACGAAGCGAATTGATTTGCTTATTGAAGTGGCT
GAGTTAGTCGTAAAAAAAGATAATGCTGTTAAATTTCATATATATGGAGAAGGATCTGTC
AAAGATAAAATAGCTAAAATGATTGAAGATAAAAATTTAGAAAGAAATGTTTTTCTTAAA
GGATATACAACAACTCCACAAAAATGCTTGGAAGATTTTAAATTAGTCGTTTCTACATCT
CAATATGAAGGTCAAGGGTTAAGTATGATAGAAGCAATGATTTCTAAAAGGCCTGTTGTT
GCCTTTGACATCAAATACGGACCAAGTGATTTTATAGAAGATAATAAAAATGGTTATTTA
ATAGAAAACCATAATATTAACGACATGGCTGATAAAATACTTCAGCTTGTTAATAATGAT
GTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACG
GAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAA
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1482

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SAOUHSC_00973
symbol: SAOUHSC_00973
description:
length: 493
theoretical pI: 9.34538
theoretical MW: 57274.4
GRAVY: -0.49858

⊟Function[edit | edit source]

⊞⊞TIGRFAM:
Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 117)
and 15 more
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 89.2)
sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 84.2)
Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 35.2)
colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 32.7)
PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 31.9)
Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 29.3)
D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 29)
glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 27.7)
sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 25.5)
Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 24.8)
sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 19.6)
Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 16.6)
sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03087; HMM-score: 16.4)
putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 14.8)
RNA polymerase sigma factor, SigM family (TIGR02950; HMM-score: 12.8)
TheSEED :
- Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
⊞⊞PFAM:
GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 120.3)
Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 106)
and 4 more
GT4-conflict; Family 4 Glycosyltransferase in conflict systems (PF20706; HMM-score: 16.6)
Glyco_trans_1_2; Glycosyl transferase-like (PF13524; HMM-score: 14.5)
no clan defined DUF4116; Domain of unknown function (DUF4116) (PF13475; HMM-score: 12.9)
DUF3695; Protein of unknown function (DUF3695) (PF12494; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
⊞⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.627
- Cytoplasmic Membrane Score: 0.0758
- Cell wall & surface Score: 0.0049
- Extracellular Score: 0.2922
⊞⊞
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
⊞⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010241
- TAT(Tat/SPI): 0.000295
- LIPO(Sec/SPII): 0.001551
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt: Q2FZM7 UniProt
STRING: 93061.SAOUHSC_00973 STRING

⊟Protein sequence[edit | edit source]

MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYKGNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEIEESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMADKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS

⊟Experimental data[edit | edit source]

experimentally validated: PeptideAtlas ^[2] ^[3]
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

predicted SigA promoter ^[4] : SAOUHSC_00972 > SAOUHSC_00973

⊟Regulation[edit | edit source]

regulator:

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊞Other Information[edit | edit source]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
Int J Med Microbiol: 2012, 302(2);84-7
[PubMed:22417616] [WorldCat.org] [DOI] (I p)
↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p)
↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e)
↑ ^{Jump up to: 4.0} ^4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Solmaz Sobhanifar, Liam James Worrall, Robert J Gruninger, Gregory A Wasney, Markus Blaukopf, Lars Baumann, Emilie Lameignere, Matthew Solomonson, Eric D Brown, Stephen G Withers, Natalie C J Strynadka
Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid α-glycosyltransferase.
Proc Natl Acad Sci U S A: 2015, 112(6);E576-85
[PubMed:25624472] [WorldCat.org] [DOI] (I p)

[pubmed22417616-1] Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
Int J Med Microbiol: 2012, 302(2);84-7
[PubMed:22417616] [WorldCat.org] [DOI] (I p)

[pubmed26224020-2] Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p)

[pubmed28887440-3] Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e)

[pubmed27035918-4] {Jump up to: 4.0} ^4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)

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Links