PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0906 [new locus tag: NWMN_RS05075 ]
• pan locus tag^?: SAUPAN003234000
• symbol: NWMN_0906
• pan gene symbol^?: tarM
• synonym:
• product: glycosyl transferase, group 1 family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0906 [new locus tag: NWMN_RS05075 ]
• symbol: NWMN_0906
• product: glycosyl transferase, group 1 family protein
• replicon: chromosome
• strand: +
• coordinates: 1003989..1005470
• length: 1482
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330545 NCBI
• RefSeq: YP_001331940 NCBI
• BioCyc:
• MicrobesOnline: 3706457 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAAAATATTTATGATGGTACATGAGTTAGATGTGAATAAAGGTGGTATGACCTCT
  TCGATGTTCAATAGAAGTAAAGAGTTTTATGATGCGGACATACCTGCTGATATTGTTACT
  TTCGATTACAAAGGAAACTATGATGAAATTATTAAAGCTTTGAAAAAACAAGGTAAAATG
  GATCGAAGAACGAAAATGTATAATGTATTTGAGTATTTTAAACAAATTTCAAATAATAAA
  CATTTTAAGTCTAATAAATTGTTATATAAACATATTTCAGAAAGACTAAAAAATACGATT
  GAAATTGAAGAGAGTAAAGGTATTTCAAGATATTTTGATATAACGACTGGTACATATATT
  GCCTACATTAGAAAAAGTAAATCTGAAAAAGTGATTGATTTCTTTAAAGATAATAAACGA
  ATTGAACGGTTTAGTTTTATAGATAATAAAGTGCATATGAAGGAAACATTTAATGTAGAT
  AATAAAGTTTGTTATCAAGTATTTTATGATGAAAAGGGATACCCATATATTTCAAGGAAT
  ATTAATGCTAATAATGGTGCTGTAGGTAAAACTTATGTGTTAGTTAATAAAAAAGAATTT
  AAAAACAATTTAGCACTGTGTGTTTACTATTTAGAAAAACTAATAAAAGATTCTAAAGAT
  AGTATTATGATTTGTGATGGACCAGGGAGTTTTCCAAAAATGTTTAATACAAATCATAAA
  AATGCTCAGAAATATGGCGTTATTCATGTTAATCATCATGAAAATTTCGATGATACGGGT
  GCATTTAAAAAAAGTGAGAAATATATTATTGAGAATGCGAATAAAATTAACGGTGTAATT
  GTATTAACAGAGGCACAAAGATTAGATATTCTTAATCAATTTGATGTAGAAAATATTTTC
  ACTATTAGCAATTTTGTTAAGATACATAATGCTCCAAAACATTTTCAAACTGAAAAAATC
  GTAGGTCATATTTCTAGAATGGTACCAACGAAGCGAATTGATTTGCTTATTGAAGTGGCT
  GAGTTAGTCGTAAAAAAAGATAATGCTGTTAAATTTCATATATATGGAGAAGGATCTGTC
  AAAGATAAAATAGCTAAAATGATTGAAGATAAAAATTTAGAAAGAAATGTTTTTCTTAAA
  GGATATACAACAACTCCACAAAAATGCTTGGAAGATTTTAAATTAGTCGTTTCTACATCT
  CAATATGAAGGTCAAGGGTTAAGTATGATAGAAGCAATGATTTCTAAAAGGCCTGTTGTT
  GCCTTTGACATCAAATACGGACCAAGTGATTTTATAGAAGATAATAAAAATGGTTATTTA
  ATAGAAAACCATAATATTAAGGACATGGCTGATAAAATACTTCAGCTTGTTAATAATGAT
  GTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACG
  GAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAA
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⊟Protein

⊟General

• locus tag: NWMN_0906 [new locus tag: NWMN_RS05075 ]
• symbol: NWMN_0906
• description: glycosyl transferase, group 1 family protein
• length: 493
• theoretical pI: 9.4155
• theoretical MW: 57288.5
• GRAVY: -0.499391

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 117.2)
  and 15 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 89.5)
  sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 84.2)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 35.3)
  colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 33)
  PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 32.1)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 29.3)
  D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 29.2)
  glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 28)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 24.8)
  sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 24.7)
  sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 19.3)
  Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 16.8)
  sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03087; HMM-score: 16.4)
  putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 14.8)
  RNA polymerase sigma factor, SigM family (TIGR02950; HMM-score: 12.8)
• TheSEED: data available for COL, NCTC8325, USA300_FPR3757
• PFAM:
  GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 113.4)
  and 4 more
  Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 88.9)
  Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 32.3)
  no clan defined DUF4116; Domain of unknown function (DUF4116) (PF13475; HMM-score: 13.9)
  DUF3695; Protein of unknown function (DUF3695) (PF12494; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010241
  • TAT(Tat/SPI): 0.000295
  • LIPO(Sec/SPII): 0.001551
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221118 NCBI
• RefSeq: YP_001331940 NCBI
• UniProt: A0A0H3K7W9 UniProt

⊟Protein sequence

• MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYKGNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEIEESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNIKDMADKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0905 > NWMN_0906

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Solmaz Sobhanifar, Liam James Worrall, Robert J Gruninger, Gregory A Wasney, Markus Blaukopf, Lars Baumann, Emilie Lameignere, Matthew Solomonson, Eric D Brown, Stephen G Withers, Natalie C J Strynadka
Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid α-glycosyltransferase.
Proc Natl Acad Sci U S A: 2015, 112(6);E576-85
[PubMed:25624472] [WorldCat.org] [DOI] (I p)