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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1981 [new locus tag: SACOL_RS10355 ]
  • pan locus tag?: SAUPAN004953000
  • symbol: SACOL1981
  • pan gene symbol?: pncA
  • synonym:
  • product: isochorismatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1981 [new locus tag: SACOL_RS10355 ]
  • symbol: SACOL1981
  • product: isochorismatase
  • replicon: chromosome
  • strand: +
  • coordinates: 2044944..2045504
  • length: 561
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3238234 NCBI
  • RefSeq: YP_186805 NCBI
  • BioCyc: see SACOL_RS10355
  • MicrobesOnline: 913459 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACACATCGCGCACTATTAGTTGTTGACTATTCATATGACTTTATCGCAGACGACGGC
    TTACTAACATGCGGTAAACCTGGACAAAATATTGAAGATTTTATTGTTTCTCGTATCAAT
    GACTTTAATTATTATCAAGACCATATATTCTTTTTAATGGATTTACATTATTTACATGAC
    ATTCATCATCCTGAAAGTAAATTATTCCCACCACACAATATCGTAGATACAAGTGGTAGA
    GAATTATACGGTAAAGTAGGTAAATTATACGAAACAATTAAAGCGCAACCTAATGTACAT
    TTCATTGATAAAACGCGCTATGATTCGTTCTTTGGTACCCCGCTTGATAGTTTATTGAGA
    GAAAGAAGTATTAATCAAGTCGAAATCGTTGGTGTATGTACCGATATTTGCGTGTTACAT
    ACAGCAATTTCTGCATACAACTTAGGTTATAAAATTTCAGTACCTGCTGAGGGAGTGGCT
    TCATTTAATCAAAAAGGGCATGAATGGGCACTTGCACATTTCAAAAACTCATTAGGTGCA
    GAGGTAGAACAACACGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    561

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1981 [new locus tag: SACOL_RS10355 ]
  • symbol: SACOL1981
  • description: isochorismatase
  • length: 186
  • theoretical pI: 6.05943
  • theoretical MW: 21198.8
  • GRAVY: -0.187097

⊟Function[edit | edit source]

  • TIGRFAM:
    pyrimidine utilization protein B (TIGR03614; HMM-score: 41.5)
  • ⊞TheSEED  :
    • Nicotinamidase (EC 3.5.1.19)
    Cofactors, Vitamins, Prosthetic Groups, Pigments NAD and NADP NAD and NADP cofactor biosynthesis global  Nicotinamidase (EC 3.5.1.19)
    and 1 more
    Stress Response Oxidative stress Redox-dependent regulation of nucleus processes  Nicotinamidase (EC 3.5.1.19)
  • PFAM:
    no clan defined Isochorismatase; Isochorismatase domain (PF00857; HMM-score: 102.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9883
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0116
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006109
    • TAT(Tat/SPI): 0.000245
    • LIPO(Sec/SPII): 0.004608
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 57650671 NCBI
  • RefSeq: YP_186805 NCBI
  • UniProt: A0A0H2WX58 UniProt

⊟Protein sequence[edit | edit source]

  • MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNYYQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFIDKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVASFNQKGHEWALAHFKNSLGAEVEQHV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell: 140 [3]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SACOL1981 > ppaC

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL1981&oldid=86946"
  • This page was last edited on 11 March 2016, at 06:05.
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