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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1054 [new locus tag: SA_RS05985 ]
  • pan locus tag?: SAUPAN003493000
  • symbol: SA1054
  • pan gene symbol?: coaBC
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1054 [new locus tag: SA_RS05985 ]
  • symbol: SA1054
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1192089..1193288
  • length: 1200
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAGAAAATATTATTAGCCGTTACAGGTGGCATTGCGGCATATAAAGCAATTGATTTG
    ACAAGTAAGTTAACACAATCTGGGTATGAAGTTCGCGTTATGTTAACGAATCACGCACAA
    AAATTTGTGACACCATTAGCATTTCAAGCAATAAGTCGAAATGCTGTTTATACAGATACT
    TTTATAGAAGAAAATCCTTCAGAAATACAGCATATTGCATTAGGTGATTGGGCAGATGCA
    ATCATTGTTGCACCTGCAACGGCAAATACAATTGCAAAATTGAGTGTAGGTATTGCTGAT
    GATTTGGTGACATCAACGTTGCTAGCAACAGAGACACCGAAATTTATTGCGCCTGCTATG
    AATGTGCATATGTATGAAAATAAACGTACGCAGCAAAATATTAATATTTTAAAAGAAGAT
    GGGTATCATTTTATCGAACCAGGAAGCGGATTTCTAGCATGTGGTTATGTTGCTAAAGGA
    CGTATGGAAGAACCGCTTCAAATCGTTTCTGTTATTGATGCTCATTTTCAAAATAGTAAT
    CGTTTAGCTAATAGTTCATTTCAAGATAAACGCGCATTGGTTACAGCAGGACCAACTATT
    GAAGTTATCGATCCAGTCAGATTTGTATCCAATCGTTCTTCTGGAAAAATGGGCTATGCA
    ATAGCTGAAGCATTGCGAAATCGAGGAGCTATCGTGACGTTAGTTGCTGGTCCTACAACA
    CTAGAGGATCCAAAAGATATTGAAGTTATTCATGTTCAAAGTGCTGAAGAAATGTTTGAA
    CAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGAC
    TATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGGTGATTTGTCAGTA
    TCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTAT
    TTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTACGC
    AAGAAAAATGCAGATGTGATTATTTCAAATAATGTTGGGGATATGTCTATCGGATTTAGT
    TCTGATGATAATGAATTGACAATGCATTTTAAAAATAATGAAAAGGTAAATATCAAGAAA
    GGAAAAAAAGTAGTATTAGCTGCACAAATTTTAGATGAACTAGAAACTAGGTGGCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1054 [new locus tag: SA_RS05985 ]
  • symbol: SA1054
  • description: hypothetical protein
  • length: 399
  • theoretical pI: 5.87138
  • theoretical MW: 44140
  • GRAVY: -0.211278

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.3.2.5?  ExPASy
    Phosphopantothenate--cysteine ligase (CTP) CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine
    EC 4.1.1.36?  ExPASy
    Phosphopantothenoylcysteine decarboxylase N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2
  • ⊞TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase (TIGR00521; EC 4.1.1.36,6.3.2.5; HMM-score: 478.1)
    and 6 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase (TIGR02113; EC 4.1.1.36; HMM-score: 208.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenate--cysteine ligase (TIGR02114; EC 6.3.2.5; HMM-score: 72.2)
    Metabolism Energy metabolism Electron transport archaeoflavoprotein AfpA (TIGR02699; HMM-score: 32.3)
    Unknown function General archaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 31.4)
    Unknown function Enzymes of unknown specificity polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases (TIGR00421; EC 4.1.1.-; HMM-score: 27.7)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, B subunit (TIGR02852; HMM-score: 16.4)
  • ⊞TheSEED  :
    • Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
    • Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)
  • ⊞PFAM:
    NADP_Rossmann (CL0063) DFP; DNA / pantothenate metabolism flavoprotein C-terminal (PF04127; HMM-score: 237.4)
    and 7 more
    no clan defined Flavoprotein; Flavoprotein (PF02441; HMM-score: 128.4)
    NADP_Rossmann (CL0063) YjeF_N; YjeF-related protein N-terminus (PF03853; HMM-score: 17.7)
    adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 16.7)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 16.7)
    no clan defined Cwp8_D2; Cell wall binding protein Cwp8 domain 2 (PF22093; HMM-score: 12.9)
    NADP_Rossmann (CL0063) DpaA_N; Dipicolinate synthase subunit A N-terminal domain (PF16924; HMM-score: 12.8)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FMN
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9982
    • Cytoplasmic Membrane Score: 0.0006
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0009
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.128674
    • TAT(Tat/SPI): 0.000733
    • LIPO(Sec/SPII): 0.04171
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVTPLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDLVTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKGRMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMGYAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAAAVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENYAQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILDELETRWQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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