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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0732 [new locus tag: SA_RS04165 ]
  • pan locus tag?: SAUPAN002711000
  • symbol: SA0732
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0732 [new locus tag: SA_RS04165 ]
  • symbol: SA0732
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 839200..839658
  • length: 459
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATC
    GTATTCTCAATATTATTGATTATTCATTACTTTGTTTCGTTGGATAATGCGACTGCCAAA
    GCATTACTTAATTTAACGAATCAAAACACTTCAGATAAAGCGATTGATTACATTTTAAAC
    AGCTTTAGATTCACTGGTATTATGTATATTTTGGCTTATCTAGCAGGCTTCATCACTTTT
    TGGAATCGACATACTTATGTGTGGTGGTTTATGTTTGCAGTTTATGTATCAAATAGTTTG
    TTTACGTTGATTAATTTATCAATCACAATTCAAGCAATAAAAGCTGCACACGGTGCGTAC
    TTAACATTGCCAATTTTAATCGTTATTATAGGTTCGGTTGCATTAGCGATTTATATGCTT
    GTTGTTTCTATCAAACGTAAAAGTACATTTAATCGCTAG
    60
    120
    180
    240
    300
    360
    420
    459

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0732 [new locus tag: SA_RS04165 ]
  • symbol: SA0732
  • description: hypothetical protein
  • length: 152
  • theoretical pI: 10.3529
  • theoretical MW: 17242.3
  • GRAVY: 0.698026

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01108117: hypothetical protein
  • ⊞PFAM:
    TSPAN_4TM-like (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 16.8)
    and 3 more
    no clan defined DUF805; Protein of unknown function (DUF805) (PF05656; HMM-score: 9.8)
    DUF373; Domain of unknown function (DUF373) (PF04123; HMM-score: 9.1)
    DUF2721; Protein of unknown function (DUF2721) (PF11026; HMM-score: 8.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.9718
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0277
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.359003
    • TAT(Tat/SPI): 0.008734
    • LIPO(Sec/SPII): 0.00174
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLNLTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFTLINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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