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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07375 [old locus tag: NWMN_1310 ]
  • pan locus tag?: SAUPAN003812000
  • symbol: NWMN_RS07375
  • pan gene symbol?: alr2
  • synonym:
  • product: alanine racemase 2

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07375 [old locus tag: NWMN_1310 ]
  • symbol: NWMN_RS07375
  • product: alanine racemase 2
  • replicon: chromosome
  • strand: +
  • coordinates: 1442694..1443779
  • length: 1086
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1442694..1443779) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1310

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGACAGCAACATGGTCTGTAAATAAGAAAATATTTTTACAAAATGCAATCACAGTCAAA
    AACAATCAGCCATTAATGGCAGTTGTTAAAAATAATGCATATCACTATGACCTAGAATTT
    GCTGTAACTCAGTTTATCCATGCAGGTATAGATACATTTAGCACAACATCACTACGAGAA
    GCAATTCAAATTAGACAACTTGCTCCAGATGCAACAATCTTTTTAATGAATGCAGTTTAC
    GAGTTTGATTTAGTCCGTGAACATCAAATACACATGACTTTGCCGTCGTTGACATATTAC
    TATAACCATAAAAATGATTTAGCTGGTATTCATGTTCACTTAGAATTTGAAAATTTATTA
    CATCGGTCTGGATTTAAAGATTTAAACGAAATTAAAGAAGTATTGAAAGATCACCATCAT
    AATCAAAATGCAAAAATGATTATTAGTGGTTTATGGACCCATTTTGGATATGCTGATGAA
    TTCGATGTGTCAGATTATAATGTTGAACGTTCACAATGGATGGAAATTGTTGAAGCACTT
    TTATCTGAAGGTTATCAGTTCGACCTAATCCATGCTCAAAATAGTGCGAGTTTTTATCGG
    GAAGGACAAATATTACTACCCCACCATACACATGCGCGTGTAGGTATTGCGTTATACGGT
    TCAAGACCATATAGTTCACTGAATCAACATGATATAGTTCAGTCATTAACTTTAAAAGCA
    CATGTTATTCAAGTGCGCGAAGTACAAGCTGGTGATTATTGCGGTTATAGCTTTGCCTTT
    GAAGTGACTAAAAACAATACAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATT
    TTAAGAACTCGTGCTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCA
    TTAATGATGAGCCATATGTTTGTTGAAGTAGATGGCAATGTACATGCACAAGATGAAGTT
    ATTCTTTATAATAATGATATCCGCATCGATGAATATACCTTTAAAGGTGTTGGTGCAAAT
    TCTGAACAATTAAGTGCTATGAATCATGATTCTTTAAAAAAGGAGTACATTTCAAATGAC
    TGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1086

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS07375 [old locus tag: NWMN_1310 ]
  • symbol: NWMN_RS07375
  • description: alanine racemase 2
  • length: 361
  • theoretical pI: 6.43166
  • theoretical MW: 41409.5
  • GRAVY: -0.29723

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 5.1.1.1?  ExPASy
    Alanine racemase L-alanine = D-alanine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan alanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 148.9)
  • TheSEED: see NWMN_1310
  • ⊞PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 125.7)
    and 1 more
    ALR_C-like (CL0866) Ala_racemase_C; Alanine racemase, C-terminal domain (PF00842; HMM-score: 67.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9979
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0019
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002434
    • TAT(Tat/SPI): 0.000132
    • LIPO(Sec/SPII): 0.000473
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQFIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYNHKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADEFDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIALYGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGYGDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTFKGVGANSEQLSAMNHDSLKKEYISNDC

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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