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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS02825 [old locus tag: NWMN_0483 ]
  • pan locus tag?: SAUPAN002286000
  • symbol: NWMN_RS02825
  • pan gene symbol?: nupC
  • synonym:
  • product: pyrimidine nucleoside transporter NupC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS02825 [old locus tag: NWMN_0483 ]
  • symbol: NWMN_RS02825
  • product: pyrimidine nucleoside transporter NupC
  • replicon: chromosome
  • strand: -
  • coordinates: 555714..556928
  • length: 1215
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (555714..556928) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0483

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCATATTCTAATTGGGATTATCGGAATTATCTTCTTTTTAGCACTTGCGTTTCTATTT
    AGCTCAGACAGGAAAAATATTCGCTGGAAATATGTAGGAATTTTACTTGCAATTCAGCTT
    ATCTTTGCATTTATCTTGCTTAAAACTACAATGGGAATCAAGGTTATTGGTGGTATTTCA
    AACGGTTTTAACTACTTATTATTAAAAGCAGCAGATGGTGTTAACTTTGTATTCGGTGGT
    ATTCAATATATCGATCCTAAGCAACCACCATTCTTCTTCAGTGTATTATTACCTATCGTA
    TTTATTTCAGCGATTATCGGTATCCTTCAATACACAAAGATTTTACCTTTAATCATTAAT
    GTTTTAGGATTCTTAATTTCTAAAATTAATGGTATGGGACGTTTAGAATCATACAATGCA
    GTTGCTGCAGCAATTTTGGGACAATCTGAAGTATTTATTTCAATTAAAAAACAATTACCA
    TACATACCTAAGCAACGTTTATACACATTAACAGCTTCAGCAATGTCGACTGTTTCTGCT
    TCAATTATCGGTGCATACTTTACCTTAATTGAACCTAAATATGTTGTAACGGCTGTTGTC
    TTAAACTTATTTGGTGGTTTCATCATCGCTTCAATTATTAACCCTTATAAAGTTAATGAA
    GAAGATGACAAATTATTAGTTGAAGAAACTGAAGAAAGACAACAATCATTCTTCGAAATG
    CTAGGAGAATATATTTTAGATGGATTTAAAGTAGCTGTCATTGTAGGCGCAATGTTAATC
    GGATACATTGCCATTATCGCTCTATTAAATGGAATTGTAAGCAATATCTTTAGTACAGTT
    TCAGGTGGCGCTATTTCTTGGGATTTCCAAACATTAATTGGTTTTGTCTTTGCACCATTT
    GCATTCTTAGTCGGTGTGCCATGGCAAGATGCAGTTCAAGCCGGTTCAGTTATGGCGACA
    AAACTATTATCTAACGAATTTGTAGCAATGCAAGCACTTGGTAAACTTAGTGATTTATCT
    GAACACGCAAAAGGTGTTACATCTGTATTCTTAGTATCATTTGCTAACTTTAGCTCTATC
    GGTATTATTTCAGGTGCGATTAAATCACTTAATGACAAAAAAGGAGATACAGTTGCCCGC
    TTTGGTTTGAAATTATTATTCGGTGCAACACTAGTATCTTTCATTTCAGCTGCAATCGCT
    GGATTCTTTATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1215

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS02825 [old locus tag: NWMN_0483 ]
  • symbol: NWMN_RS02825
  • description: pyrimidine nucleoside transporter NupC
  • length: 404
  • theoretical pI: 9.44764
  • theoretical MW: 43804.7
  • GRAVY: 0.870792

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside transporter, NupC family (TIGR00804; HMM-score: 433.4)
  • TheSEED: see NWMN_0483
  • ⊞PFAM:
    no clan defined Nucleos_tra2_C; Na+ dependent nucleoside transporter C-terminus (PF07662; HMM-score: 214.4)
    and 2 more
    Nucleos_tra2_N; Na+ dependent nucleoside transporter N-terminus (PF01773; HMM-score: 79.4)
    Gate; Nucleoside recognition (PF07670; HMM-score: 50)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9997
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0003
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011503
    • TAT(Tat/SPI): 0.000267
    • LIPO(Sec/SPII): 0.060914
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

  • GI: 446469940 NCBI
  • RefSeq: WP_000547794 NCBI
  • UniProt: see NWMN_0483

⊟Protein sequence[edit | edit source]

  • MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAFILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFISAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLPYIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKVNEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNIFSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALGKLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLVSFISAAIAGFFI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA see NWMN_0483

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_RS02825&oldid=72302"
  • This page was last edited on 10 March 2016, at 21:39.
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