PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_0800 [new locus tag: NWMN_RS04525 ]
pan locus tag^?: SAUPAN003027000
symbol: NWMN_0800
pan gene symbol^?: nagD
• synonym:
• product: hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0800 [new locus tag: NWMN_RS04525 ]
• symbol: NWMN_0800
• product: hydrolase
• replicon: chromosome
• strand: +
• coordinates: 888714..889493
• length: 780
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330473 NCBI
• RefSeq: YP_001331834 NCBI
• BioCyc:
• MicrobesOnline: 3706349 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAACAGTATAAAGCGTATTTAATCGATTTAGATGGCACAATGTATATGGGAACAGAT
  GAGATTGATGGAGCAAAACAATTCATCGATTATTTAAATGTAAAAGGCATTCCTCATTTA
  TACGTAACTAATAATTCAACAAAAACACCTGAGCAAGTAACTGAAAAATTACGTGAAATG
  CACATTGATGCTAAACCAGAAGAGGTTGTAACGTCAGCGTTAGCCACTGCTGATTATATT
  TCAGAACAATCACCAGGAGCATCAGTATATATGTTAGGTGGGAGTGGTTTAAATACTGCG
  TTAACCGAAGCGGGACTTGTCATTAAAAATGACGAGCATGTTGATTATGTAGTTATTGGA
  CTTGACGAACAAGTTACATATGAAAAGCTTGCGATTGCAACGTTAGGTGTAAGAAATGGT
  GCAACATTTATTTCTACAAATCCTGATGTATCAATTCCTAAAGAGCGTGGTTTATTACCT
  GGTAATGGTGCTATTACAAGTGTTGTAAGTGTATCGACAGGTGTATCGCCACAATTTATT
  GGTAAACCAGAACCGATTATTATGGTTAAAGCATTAGAAATTTTAGGATTAGATAAATCC
  GAAGTTGCTATGGTAGGCGATTTGTACGATACCGATATTATGTCTGGTATTAACGTAGGT
  ATGGATACGATTCATGTACAAACAGGTGTATCTACGTTAGAAGATGTGCAAAATAAAAAT
  GTGCCACCAACGTATTCTTTTAAAGATTTAAATGAAGCAATAGCTGAATTAGAAAAATAG
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⊟Protein

⊟General

• locus tag: NWMN_0800 [new locus tag: NWMN_RS04525 ]
• symbol: NWMN_0800
• description: hydrolase
• length: 259
• theoretical pI: 4.22967
• theoretical MW: 27945.5
• GRAVY: -0.0791506

⊟Function

• reaction:
  EC 3.1.3.-?  ExPASy
• ⊞TIGRFAM:
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01457 family (TIGR01457; HMM-score: 328.9)
  and 21 more
  phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 145.3)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 133)
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 75.7)
  HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 59.6)
  HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 40.9)
  noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 36.5)
  HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 31.4)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 27.2)
  HAD hydrolase, TIGR01456 family (TIGR01456; HMM-score: 21.6)
  histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 21.5)
  HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 19.8)
  AHBA synthesis associated protein (TIGR01454; HMM-score: 19.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides 5'-nucleotidase, lipoprotein e(P4) family (TIGR01533; HMM-score: 19.3)
  Metabolism Transport and binding proteins Other 5'-nucleotidase, lipoprotein e(P4) family (TIGR01533; HMM-score: 19.3)
  pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 17.4)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 16.5)
  phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 15.9)
  Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 15)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 14.2)
  HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 12.4)
  Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 12)
• TheSEED  :
  • Hypothetical NagD-like phosphatase
• ⊞PFAM:
  HAD (CL0137) Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 92.6)
  and 4 more
  Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 71.2)
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 70.5)
  HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 40.2)
  PGP_phosphatase; Mitochondrial PGP phosphatase (PF09419; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• ⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9956
  • Cytoplasmic Membrane Score: 0.0009
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0031
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005465
  • TAT(Tat/SPI): 0.000134
  • LIPO(Sec/SPII): 0.000695
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221012 NCBI
• RefSeq: YP_001331834 NCBI
• UniProt: A0A0H3K7Q5 UniProt

⊟Protein sequence

• MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTNNSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALTEAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLPGNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGINVGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0799 > NWMN_0800 > NWMN_0801

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications