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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07500 [old locus tag: SA1322 ]
  • pan locus tag?: SAUPAN004024000
  • symbol: SA_RS07500
  • pan gene symbol?: srrB
  • synonym:
  • product: two-component sensor histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07500 [old locus tag: SA1322 ]
  • symbol: SA_RS07500
  • product: two-component sensor histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1530839..1532590
  • length: 1752
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1530839..1532590) NCBI
  • BioCyc: SA_RS07500 BioCyc
  • MicrobesOnline: see SA1322

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGAGCCGGCTAAATAGTGTCGTAATTAAACTGTGGTTAACTATTATTTTAATAGTG
    ACGACAGTTTTAATTTTATTAAGTATTGCTTTAATTACCTTTATGCAATACTATTTCACA
    CAAGAAACCGAAAATGCCATAAGAGAAGATGCTAGACGTATAAGTTCACTGGTCGAACAA
    TCACATAATAAAGAAGAAGCAATAAAATATAGTCAAACATTAATTGAAAATCCTGGTGGG
    TTGATGATTATAAATAATAAACATCGTCAATCAACGGCTTCACTTTCTAATATTAAAAAG
    CAAATGTTGAATGAAGTAGTCAACAACGACCATTTTGACGATGTGTTTGATAAAGGTAAA
    TCTGTTACTCGAAATGTAACGATTAAAGAAAAGGGTTCATCTCAAACATATATTTTGTTA
    GGCTATCCAACAAAAGCACAGAAGAATAGTCATAGCAAATATAGTGGAGTCTTTATATAT
    AAAGACTTGAAATCAATCGAAGATACAAATAATGCTATTACGATTATCACTATAATTACG
    GCTGTTATTTTCTTAACAATTACAACAGTCTTTGCGTTTTTCTTATCGTCAAGAATTACA
    AAACCTTTAAGACGTTTAAGAGACCAAGCTACACGTGTATCTGAAGGGGATTACTCTTAT
    AAACCTTCTGTCACAACGAAAGATGAAATTGGTCAATTATCGCAGGCATTTAATCAGATG
    AGTACAGAAATCGAAGAGCATGTCGACGCATTATCCACATCTAAAAATATTAGAGACAGC
    TTAATTAACTCTATGGTAGAAGGTGTCCTAGGTATTAATGAGAGTCGACAAATTATCTTA
    TCTAATAAGATGGCGAATGATATTATGGACAATATTGATGAAGATGCTAAAGCTTTCTTA
    TTAAGACAAATTGAAGATACTTTTAAATCAAAACAAACGGAAATGCGTGATTTAGAAATG
    AATGCACGATTCTTTGTTGTGACCACAAGCTATATCGATAAGATTGAACAGGGAGGTAAA
    AGTGGTGTTGTTGTGACAGTTCGTGATATGACTAATGAGCACAATCTAGATCAAATGAAG
    AAAGATTTCATTGCTAATGTATCACATGAATTACGAACACCTATATCATTACTACAAGGT
    TATACTGAATCAATTGTAGATGGTATTGTTACAGAACCGGATGAAATAAAAGAATCGCTC
    GCCGTTGTCCTTGATGAATCGAAACGTTTAAATCGATTAGTTAATGAATTGTTAAATGTC
    GCACGCATGGATGCTGAAGGGTTATCCGTAAATAAAGAAGTTCAGCCTATTGCAGCGTTA
    CTAGATAAGATGAAAATTAAGTATCGCCAACAAGCTGATGATTTAGGTCTAAATATGACT
    TTTAATTATTGTAAGAAGCGTGTTTGGAGTTATGATATGGATCGCATGGACCAAGTACTA
    ACGAACTTAATTGATAATGCATCACGTTATACGAAACCTGGAGATGAAATTGCAATTACT
    TGTGATGAAAATGAAAGCGAAGATATTTTATACATTAAAGATACAGGCACAGGCATTGCA
    CCAGAACATTTACAACAAGTATTTGATCGTTTTTATAAAGTTGATGCAGCGAGAACGCGA
    GGTAAACAAGGTACCGGTTTAGGTTTGTTCATTTGTAAAATGATTATCGAAGAGCATGGT
    GGTTCCATTGATGTTAAAAGCGAATTAGGAAAAGGCACAACATTTATTATTAAACTACCA
    AAACCAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1752

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS07500 [old locus tag: SA1322 ]
  • symbol: SA_RS07500
  • description: two-component sensor histidine kinase
  • length: 583
  • theoretical pI: 5.76595
  • theoretical MW: 66061.1
  • GRAVY: -0.300515

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • ⊞TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 242.1)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 199.6)
    and 6 more
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 135.4)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 96.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.8)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.8)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.7)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.7)
  • TheSEED: see SA1322
  • ⊞PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 105)
    and 13 more
    Cache (CL0165) HisK_sensor; Histidine kinase sensor domain (PF18698; HMM-score: 80.5)
    His_Kinase_A (CL0025) HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 67.5)
    HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 65.6)
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 24.5)
    no clan defined Halogen_Hydrol; 5-bromo-4-chloroindolyl phosphate hydrolysis protein (PF10112; HMM-score: 17.2)
    PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 16.7)
    no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 13.3)
    Vpu; Vpu protein (PF00558; HMM-score: 13.2)
    TPR (CL0020) DUF6377; Domain of unknown function (DUF6377) (PF19904; HMM-score: 12.7)
    no clan defined CD34_antigen; CD34/Podocalyxin family (PF06365; HMM-score: 10.5)
    TSPAN_4TM-like (CL0347) Tetraspanin; Tetraspanin family (PF00335; HMM-score: 10.4)
    no clan defined CemA; CemA family (PF03040; HMM-score: 7.7)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 6.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9997
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0003
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002704
    • TAT(Tat/SPI): 0.000289
    • LIPO(Sec/SPII): 0.00216
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETENAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQMLNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIYKDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDYSYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESRQIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDKIEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTEPDEIKESLAVVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQQADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESEDILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVKSELGKGTTFIIKLPKPE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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