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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1441 [new locus tag: SAUSA300_RS07870 ]
  • pan locus tag?: SAUPAN004024000
  • symbol: srrB
  • pan gene symbol?: srrB
  • synonym:
  • product: respiratory response protein, SrrB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1441 [new locus tag: SAUSA300_RS07870 ]
  • symbol: srrB
  • product: respiratory response protein, SrrB
  • replicon: chromosome
  • strand: -
  • coordinates: 1595174..1596940
  • length: 1767
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    TTGAGGTTAAATCTAATGATGAGCCGGCTAAATAGTGTCGTAATTAAACTGTGGTTAACT
    ATTATTTTAATAGTGACGACAGTTTTAATTTTATTAAGTATTGCTTTAATTACCTTTATG
    CAATACTATTTCACACAAGAAACCGAAAATGCCATAAGAGAAGATGCTAGACGTATAAGT
    TCACTGGTCGAACAATCACATAATAAAGAAGAAGCAATAAAATATAGTCAAACATTAATT
    GAAAATCCTGGTGGGTTGATGATTATAAATAATAAACATCGTCAATCAACGGCTTCACTT
    TCTAATATTAAAAAGCAAATGTTGAATGAAGTAGTCAACAACGACCATTTTGACGATGTG
    TTTGATAAAGGTAAATCTGTTACTCGAAATGTAACGATTAAAGAAAAGGGCTCATCTCAA
    ACATATATTTTGTTAGGCTATCCAACAAAAGCACAGAAGAATAGTCATAGCAAATATAGT
    GGAGTCTTTATATATAAAGACTTGAAATCAATCGAAGATACAAATAATGCTATTACGATT
    ATCACCATAATTACGGCTGTTATTTTCTTAACAATTACAACAGTCTTTGCGTTTTTCTTA
    TCGTCAAGAATTACAAAACCTTTAAGACGTTTAAGAGACCAAGCTACACGTGTATCTGAA
    GGGGATTACTCTTATAAACCTTCTGTCACAACGAAAGATGAAATTGGTCAATTATCGCAG
    GCATTTAATCAGATGAGTACAGAAATCGAAGAGCATGTCGACGCATTATCCACATCTAAA
    AATATTAGAGACAGCTTAATTAACTCTATGGTAGAAGGTGTCCTAGGTATTAATGAGAGT
    CGACAAATTATCTTATCTAATAAGATGGCGAATGATATTATGGACAATATTGATGAAGAT
    GCTAAAGCTTTCTTATTAAGACAAATAGAAGATACTTTTAAATCAAAACAAACTGAAATG
    CGCGATTTAGAAATGAATGCACGATTCTTTGTTGTGACCACAAGCTATATCGACAAGATT
    GAACAGGGAGGTAAAAGTGGTGTTGTTGTGACAGTTCGTGATATGACTAATGAGCACAAT
    CTAGATCAAATGAAGAAAGATTTCATTGCTAATGTATCACATGAATTACGTACACCGATA
    TCATTACTTCAAGGTTATACTGAATCAATTGTAGATGGTATTGTTACAGAACCGGATGAA
    ATAAAAGAATCGCTTGCCATTGTCCTTGATGAATCGAAACGTTTAAATCGTTTAGTTAAT
    GAATTGTTAAATGTCGCACGCATGGATGCTGAAGGGTTATCCGTAAATAAAGAAGTTCAG
    CCTATTGCAGCGTTACTAGATAAGATGAAAATTAAGTATCGCCAACAAGCTGATGATTTA
    GGTCTAAATATGACTTTTAATTATTGTAAGAAGCGTGTTTGGAGTTATGATATGGATCGC
    ATGGACCAAGTACTAACGAACTTAATTGATAATGCATCACGTTATACGAAACCTGGAGAT
    GAAATTGCAATTACTTGTGATGAAAATGAAAGCGAAGATATTTTATACATTAAAGATACA
    GGTACAGGCATTGCACCAGAACATTTACAACAAGTATTTGATCGTTTTTATAAAGTTGAT
    GCAGCGAGAACGCGAGGTAAACAAGGTACCGGTTTAGGTTTGTTCATTTGTAAAATGATT
    ATCGAAGAGCATGGTGGTTCCATAGATGTTAAAAGCGAATTAGGGAAAGGCACAACATTT
    ATTATTAAACTACCAAAACCAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1767

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1441 [new locus tag: SAUSA300_RS07870 ]
  • symbol: SrrB
  • description: respiratory response protein, SrrB
  • length: 588
  • theoretical pI: 5.93902
  • theoretical MW: 66703
  • GRAVY: -0.294898

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.13.3  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine?
  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 242.7)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 199.2)
    and 6 more
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 135)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 95.6)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.6)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.6)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.3)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.3)
  • TheSEED  :
    • Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)
    Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 95.1)
    and 17 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 64.9)
    no clan defined HAMP; HAMP domain (PF00672; HMM-score: 62.8)
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 23.4)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 18.8)
    no clan defined Halogen_Hydrol; 5-bromo-4-chloroindolyl phosphate hydrolysis protein (PF10112; HMM-score: 18.6)
    PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 16.8)
    no clan defined Cep57_MT_bd; Centrosome microtubule-binding domain of Cep57 (PF06657; HMM-score: 14.5)
    EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 13.5)
    Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 12.5)
    DUF3328; Domain of unknown function (DUF3328) (PF11807; HMM-score: 12.3)
    Vpu; Vpu protein (PF00558; HMM-score: 12.2)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 10.8)
    no clan defined Serinc; Serine incorporator (Serinc) (PF03348; HMM-score: 9.4)
    PspB; Phage shock protein B (PF06667; HMM-score: 7.8)
    CemA; CemA family (PF03040; HMM-score: 7.1)
    DUF3165; Protein of unknown function (DUF3165) (PF11364; HMM-score: 6.6)
    TRP; Transient receptor potential (TRP) ion channel (PF06011; HMM-score: 5.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00135
    • TAT(Tat/SPI): 0.000222
    • LIPO(Sec/SPII): 0.001868
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRLNLMMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETENAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQMLNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIYKDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDYSYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESRQIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDKIEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTEPDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQQADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESEDILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVKSELGKGTTFIIKLPKPE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Rex (repression) regulon
    Rex(TF)important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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