⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS01445 [old locus tag: SA0245 ]
pan locus tag^?: SAUPAN001133000
symbol: SA_RS01445
pan gene symbol^?: tarI
• synonym:
• product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS01445 [old locus tag: SA0245 ]
• symbol: SA_RS01445
• product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
• replicon: chromosome
• strand: +
• coordinates: 298241..298957
• length: 717
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (298241..298957) NCBI
• BioCyc: SA_RS01445 BioCyc
• MicrobesOnline: see SA0245

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAATACGCTGGTATTCTAGCTGGAGGTATAGGCTCAAGAATGGGTAACGTACCTTTA
  CCTAAACAATTTTTAGATTTAGACAACAAACCGATTTTAATCCATACATTAGAAAAATTT
  ATTTTAATTAATGATTTTGAAAAAATTATTATCGCGACGCCACAACAATGGATGACGCAT
  ACGAAAGATACACTTAGAAAATTCAAAATTTCTGATGAAAGAATTGAAGTCATTCAAGGT
  GGTAGCGATCGTAACGATACAATTATGAATATCGTTAAACATATTGAATCAACAAATGGT
  ATTAACGATGACGATGTCATTGTGACACATGATGCAGTTAGACCATTTTTAACGCATCGT
  ATTATTAAAGAAAATATTCAAGCTGCTTTAGAGTACGGTGCAGTAGATACAGTGATTGAT
  GCTATAGATACGATTGTTACATCTAAAGATAATCAAACGATTGATGCAATTCCAGTGCGT
  AATGAAATGTACCAAGGTCAAACACCTCAATCGTTTAATATTAATTTATTAAAAGAAAGC
  TATGCACAGTTGAGTGATGAGCAAAAGAGTATTTTATCTGATGCTTGTAAGATTATTGTA
  GAAACAAACAAACCGGTTCGACTTGTAAAAGGTGAGTTATATAACATTAAAGTAACAACA
  CCTTACGATTTAAAAGTAGCGAATGCTATTATTCGAGGTGGTATTGCCGATGATTAA
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  717
  

⊟Protein

⊟General

• locus tag: SA_RS01445 [old locus tag: SA0245 ]
• symbol: SA_RS01445
• description: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
• length: 238
• theoretical pI: 5.36178
• theoretical MW: 26656.4
• GRAVY: -0.215546

⊟Function

• ⊞⊞reaction:
  EC 2.7.7.40?  ExPASy
  D-ribitol-5-phosphate cytidylyltransferase CTP + D-ribitol 5-phosphate = diphosphate + CDP-ribitol
  EC 2.7.7.60?  ExPASy
  2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
• ⊞⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TIGR00453; EC 2.7.7.60; HMM-score: 156.7)
  and 8 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor guanylyltransferase (TIGR02665; EC 2.7.7.77; HMM-score: 19.6)
  molybdenum cofactor cytidylyltransferase (TIGR03310; EC 2.7.7.76; HMM-score: 19.2)
  glucose-1-phosphate thymidylyltransferase (TIGR01208; EC 2.7.7.24; HMM-score: 16.8)
  Hypothetical proteins Conserved TIGR00454 family protein (TIGR00454; HMM-score: 14.6)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (TIGR01479; EC 2.7.7.13,5.3.1.8; HMM-score: 14.5)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides glucose-1-phosphate cytidylyltransferase (TIGR02623; EC 2.7.7.33; HMM-score: 14)
  Cell structure Cell envelope Other rare lipoprotein A (TIGR00413; HMM-score: 13.4)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides glucose-1-phosphate thymidylyltransferase (TIGR01207; EC 2.7.7.24; HMM-score: 13.2)
• TheSEED: see SA0245
• ⊞⊞PFAM:
  GT-A (CL0110) IspD; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (PF01128; HMM-score: 145.8)
  and 4 more
  NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 34)
  CTP_transf_3; Cytidylyltransferase (PF02348; HMM-score: 26.9)
  NTP_transferase; Nucleotidyl transferase (PF00483; HMM-score: 18.2)
  no clan defined DUF1699; Protein of unknown function (DUF1699) (PF08004; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9652
  • Cytoplasmic Membrane Score: 0.0138
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0209
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004137
  • TAT(Tat/SPI): 0.000092
  • LIPO(Sec/SPII): 0.000995
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446795230 NCBI
• RefSeq: WP_000872486 NCBI
• UniProt: see SA0245

⊟Protein sequence

• MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILINDFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGINDDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVRNEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKVTTPYDLKVANAIIRGGIADD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• ⊟⊟interaction partners:

  SA_RS05350		pyruvate dehydrogenase E1 component subunit alpha   (data from MRSA252)
  SA_RS11680		30S ribosomal protein S5   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications