PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA2300 [new locus tag: SA_RS13185 ]
pan locus tag^?: SAUPAN006141000
symbol: SA2300
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2300 [new locus tag: SA_RS13185 ]
• symbol: SA2300
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2584066..2585343
• length: 1278
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125227 NCBI
• RefSeq: NP_375624 NCBI
• BioCyc: see SA_RS13185
• MicrobesOnline: 104650 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGAAGGAAAAACGAACTAACGTAAGGTGGATGTTCGCGCTTGCGTTCTTCTTTATTGGG
  GTTATTGCGTATATGGATAGAGCAAACATTTCATATATCGCTAAACAAATGATGGATGAT
  TTAGGGATGACCAAACCACAATTTGGTTTATTGGCATCATTCTTCTCTCTAGGTTATGCA
  TTAATGCAAGTACCATCGGGGATGTTGGCTGAAAAATTCGGTCCACGTAAGATGATTACA
  ATTGCATTAGTTTGGTGGAGTGCATTTACAATCTTAACGGGTATGATTAAGAACCACGGT
  TTAATTTATTTAGTGAGATTCTTATTTGGTGTGGGTGAGGCGCCAATGTATCCTTCTAAT
  GCTGTGTTTAACTCATTTTGGTTCTCTAAAAATGAAAAAGGTAGAGCATCAAGTGCATTA
  TTAGCAGGATCATATTTCGGACCTGTATTAGCACCAATAGTTACAATTGCTATTGTTAAC
  GCATTTAACTGGCAAGCAGTATTTTACATTTTTGGTGCAGTAGGTATTTTAATGGCTGTA
  TTATGGGCGATTATTGCCAAAGACTTACCTGAGCAACATAGAATGGTTAATGAAGCGGAG
  AAACGTTTCATTATGGAAAATCGTGATATCGTAGCTACTGAAAAGTCATCACCACCATGG
  AATAATTTCTTTAAACGTTTTAGCTTCTATGCAATTGCAATTCAATACTTTGTTGTACAA
  TTTATCATTACATTATTCTTAATTTGGTTACCGACGTATTTAACAGAAGTATTCCACGTT
  AACTTTAAAGAAATGAGCATTAGTTCATTACCTTGGTTATTAATGTTCTTCTTAATCTTA
  TCAGCAGGTGCAATTTCTGACCGTGTATTAGGATTAGGTCGTTCAAAATTCGTAGCTAGA
  GGTGTAATTGCAATCGCAGGATTTATTGTGTTTGCAGTTTCAATTATCTTTGCTGTACGT
  ACAGGAAATTTATATGTAAGTATTTTCTGGTTATCACTAGGTCTTGGTGGTATCGGTATT
  TCAATGGGTATGAGTTGGGCTGCAGCAACTGACTTAGGACGTAACTTCTCTGGTACAGTA
  TCAGGGTGGATGAACTTATGGGGTAATATAGGTGCATTAATCAGTCCGCTATTAGCAGGT
  CTATTCGTAGAACATTTAGGTTGGACAATGACATTCCAATTGTTAATCGTTCCAGCAGTA
  ATCGCTGTGATTATGTGGTTCTATGTGAAACCAGATCAACCTTTAATTGTTAGTGATGAT
  AAAGCAATAGAAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1278
  

⊟Protein

⊟General

• locus tag: SA2300 [new locus tag: SA_RS13185 ]
• symbol: SA2300
• description: hypothetical protein
• length: 425
• theoretical pI: 10.0983
• theoretical MW: 47625.3
• GRAVY: 0.676706

⊟Function

• ⊞TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 236.8)
  and 19 more
  Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 169.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 134.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 90.9)
  Metabolism Transport and binding proteins Other glycerol-3-phosphate transporter (TIGR00712; HMM-score: 72.8)
  4-hydroxyphenylacetate permease (TIGR02332; HMM-score: 64.3)
  Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 54.3)
  multidrug resistance protein (TIGR00880; HMM-score: 50.6)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 48.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 39.7)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 37.4)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 37.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid/peptide transporter (Peptide:H+ symporter) (TIGR00924; HMM-score: 33.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 32.3)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 28.8)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 24.5)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 20.3)
  Metabolism Transport and binding proteins Anions phosphate:H+ symporter (TIGR00887; HMM-score: 16.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 15.7)
  Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 13.2)
• TheSEED  :
  • Uncharacterized MFS-type transporter
• ⊞PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 220.9)
  and 3 more
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 19.6)
  no clan defined RseC_MucC; Positive regulator of sigma(E), RseC/MucC (PF04246; HMM-score: 7.5)
  DUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 6.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9992
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0008
• ⊞LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.103938
  • TAT(Tat/SPI): 0.001043
  • LIPO(Sec/SPII): 0.021064
• predicted transmembrane helices (TMHMM): 11

⊟Accession numbers

• GI: 15928091 NCBI
• RefSeq: NP_375624 NCBI
• UniProt: A0A0H3JRU7 UniProt

⊟Protein sequence

• MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMTKPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLIYLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVNAFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSPPWNNFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMFFLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLGLGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTFQLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• ⊞regulator: CcpA regulon

  CcpA

  (TF)

  important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications