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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002489
  • pan locus tag?: SAUPAN006141000
  • symbol: JSNZ_002489
  • pan gene symbol?: —
  • synonym:
  • product: MFS transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002489
  • symbol: JSNZ_002489
  • product: MFS transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2495630..2496907
  • length: 1278
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAGGAAAAACGAACTAACGTAAGGTGGATGTTCGCGCTTGCGTTCTTCTTTATTGGG
    GTTATTGCGTATATGGATAGAGCAAACATTTCATATATCGCTAAGCAAATGATGGATGAT
    TTAGGGATGACAAAACCACAATTTGGTTTATTGGCATCATTCTTCTCTCTAGGTTATGCA
    TTAATGCAAGTACCATCGGGGATGTTGGCTGAAAAATTCGGTCCACGTAAGATGATTACT
    ATTGCATTAGTTTGGTGGAGTGCATTTACAATCTTAACGGGTATGATTAAGAACCACGGT
    TTAATTTATTTAGTGAGATTCTTATTTGGTGTGGGTGAGGCGCCAATGTACCCTTCTAAT
    GCTGTGTTTAACTCATTTTGGTTCTCTAAAAATGAAAAAGGTAGAGCATCAAGTGCATTA
    TTAGCAGGATCATATTTCGGACCTGTATTAGCACCAATAGTTACAATTGCTATTGTTAAC
    GCATTTAACTGGCAAGCAGTATTTTACATTTTTGGTGCAGTAGGTATTTTAATGGCTGTA
    TTATGGGCGATTATTGCCAAAGACTTACCTGAGCAACATAGAATGGTTAATGAAGCGGAG
    AAACGTTTCATTATGGAAAATCGTGATATCGTAGCTACTGAAAAGTCATCACCACCATGG
    AATGATTTCTTTAAACGTTTTAGCTTCTATGCAATTGCAATTCAATACTTTGTTGTACAA
    TTTATCATTACATTATTCTTAATTTGGTTACCGACGTATTTAACAGAAGTATTCCACGTT
    AACTTTAAAGAAATGAGCATTAGTTCATTACCTTGGTTATTAATGTTCTTCTTAATCTTA
    TCAGCAGGTGCAATTTCTGACCGTGTATTAGGATTAGGTCGTTCAAAATTCGTAGCTAGA
    GGTGTAATTGCAATCGCAGGATTTATTGTATTTGCAGTTTCAATTATCTTTGCTGTACGT
    ACAGGAAATTTATATGTAAGTATTTTCTGGTTATCACTAGGTCTTGGTGGTATCGGTATT
    TCAATGGGTATGAGTTGGGCTGCAGCAACTGACTTAGGACGTAACTTCTCTGGTACAGTA
    TCAGGGTGGATGAACTTATGGGGTAATATAGGTGCATTAATCAGTCCGCTATTAGCAGGT
    CTATTCGTAGAACATTTAGGTTGGACAATGACATTCCAATTGTTAATCGTTCCAGCAGTA
    ATCGCTGTGATTATGTGGTTCTATGTGAAACCAGATCAACCTTTAATTGTTAGTGATGAT
    AAAGCAATAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1278

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002489
  • symbol: JSNZ_002489
  • description: MFS transporter
  • length: 425
  • theoretical pI: 10.0181
  • theoretical MW: 47626.3
  • GRAVY: 0.676706

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 236.9)
    and 19 more
    Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 169.5)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 134.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 90.9)
    Metabolism Transport and binding proteins Other glycerol-3-phosphate transporter (TIGR00712; HMM-score: 72.8)
    4-hydroxyphenylacetate permease (TIGR02332; HMM-score: 64.3)
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 54.6)
    multidrug resistance protein (TIGR00880; HMM-score: 50.6)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 47.7)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 40.2)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 37.9)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 37.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid/peptide transporter (Peptide:H+ symporter) (TIGR00924; HMM-score: 33.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 30.7)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 28.8)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 24.9)
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 20.7)
    Metabolism Transport and binding proteins Anions phosphate:H+ symporter (TIGR00887; HMM-score: 16.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 15.7)
    Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 13.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 223)
    and 3 more
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 18.9)
    no clan defined RseC_MucC; Positive regulator of sigma(E), RseC/MucC (PF04246; HMM-score: 7.5)
    DUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 6.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9992
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0008
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.103938
    • TAT(Tat/SPI): 0.001043
    • LIPO(Sec/SPII): 0.021064
  • predicted transmembrane helices (TMHMM): 11

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMTKPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLIYLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVNAFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSPPWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMFFLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLGLGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTFQLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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