PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0573 [new locus tag: SA_RS03310 ]
pan locus tag^?: SAUPAN002485000
symbol: sarA
pan gene symbol^?: sarA
• synonym:
• product: accessory regulator A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0573 [new locus tag: SA_RS03310 ]
• symbol: sarA
• product: accessory regulator A
• replicon: chromosome
• strand: -
• coordinates: 666347..666721
• length: 375
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123379 NCBI
• RefSeq: NP_373827 NCBI
• BioCyc: see SA_RS03310
• MicrobesOnline: 102853 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGCAATTACAAAAATCAATGATTGCTTTGAGTTGTTATCAATGGTCACTTATGCTGAC
  AAATTAAAAAGTTTAATTAAAAAGGAATTTTCAATTAGCTTTGAAGAATTCGCTGTATTG
  ACATACATCAGCGAAAACAAAGAGAAAGAATACTATCTTAAAGATATTATTAATCATTTA
  AACTACAAACAACCACAAGTTGTTAAAGCAGTTAAAATTTTATCTCAAGAAGATTACTTC
  GATAAAAAACGTAATGAGCATGATGAAAGAACTGTATTAATTCTTGTTAATGCACAACAA
  CGTAAAAAAATCGAATCATTATTGAGTCGAGTAAATAAACGAATCACTGAAGCAAACAAC
  GAAATTGAACTATAA
  60
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  375
  

⊟Protein

⊟General

• locus tag: SA0573 [new locus tag: SA_RS03310 ]
• symbol: SarA
• description: accessory regulator A
• length: 124
• theoretical pI: 8.4094
• theoretical MW: 14717.9
• GRAVY: -0.495968

⊟Function

• ⊞TIGRFAM:
  Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 118)
  and 6 more
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 13.3)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 13.3)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 13.3)
  homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 13)
  undecaprenyl-phosphate glucose phosphotransferase (TIGR03023; EC 2.7.8.-; HMM-score: 12.2)
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 11.3)
• TheSEED  :
  • Staphylococcal accessory regulator A (SarA)
  Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Staphylococcal accessory regulator A (SarA)
• ⊞PFAM:
  HTH (CL0123) Staph_reg_Sar_Rot; Transcriptional regulator SarA/Rot (PF22381; HMM-score: 87.2)
  and 4 more
  HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 25)
  MarR_2; MarR family (PF12802; HMM-score: 22.4)
  MarR; MarR family (PF01047; HMM-score: 19.9)
  P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9019
  • Cytoplasmic Membrane Score: 0.0091
  • Cell wall & surface Score: 0.0007
  • Extracellular Score: 0.0884
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004617
  • TAT(Tat/SPI): 0.000227
  • LIPO(Sec/SPII): 0.000605
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926294 NCBI
• RefSeq: NP_373827 NCBI
• UniProt: Q7A732 UniProt

⊟Protein sequence

• MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYISENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRKKIESLLSRVNKRITEANNEIEL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Jean-Philippe Didier, Alain J Cozzone, Bertrand Duclos
Phosphorylation of the virulence regulator SarA modulates its ability to bind DNA in Staphylococcus aureus.
FEMS Microbiol Lett: 2010, 306(1);30-6
[PubMed:20337713] [WorldCat.org] [DOI] (I p)