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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08645 [old locus tag: SACOL1695 ]
  • pan locus tag?: SAUPAN004246000
  • symbol: SACOL_RS08645
  • pan gene symbol?: queA
  • synonym:
  • product: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS08645 [old locus tag: SACOL1695 ]
  • symbol: SACOL_RS08645
  • product: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA
  • replicon: chromosome
  • strand: -
  • coordinates: 1727737..1728762
  • length: 1026
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1727737..1728762) NCBI
  • BioCyc: SACOL_RS08645 BioCyc
  • MicrobesOnline: see SACOL1695

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    GTGAATATTGAAGAATTTGACTATGACTTACCAGAATCATTAATTGCTCAAACGCCTTTA
    AAAGATCGTGATCATAGTCGTTTATTAGTCATGGATAGAGAAACTGGTGAAATGAAACAT
    TTACATTTCAAAGATATCATTGAGTATTTTAGACCTGGTGATACATTAGTGCTTAACGAT
    ACGCGAGTAATGCCAGCTAGACTTTTTGGTTTAAAAGAAGAAACTGGTGCAAAAGTTGAA
    ATGTTAATGTTAACTCAAATTGAAGGTAATGATTGGGAAGTCTTACTGAAACCAGCTAAG
    CGTATTAAAGTTGGTAATAAATTGAATTTTGGTAATGGCAAAATTATAGCTGAATGCATA
    AAAGAAATGGATCAAGGTGGACGCATCATGCGTTTACATTATGAAGGTATTTTACAAGAA
    AGATTAGATGAATTAGGGGAAATGCCACTGCCACCATACATCAAAGAACGTTTAGATGAT
    CCAGATCGTTATCAAACAGTTTACGCTAAAGAAAGTGGTTCAGCGGCAGCACCAACAGCA
    GGATTACATTTTACTGATGAGTTATTAATTGAAATTAAAAATAAAGGTGTTAATATCGCA
    TTTGTTACATTACATGTTGGGTTAGGTACGTTTAGACCGGTGAGCGTAGACGATGTGAAT
    GACCACGAAATGCATAGTGAATATTATCAAATGACACAAGAAACAGCTGATTTATTAAAT
    GATACTAAGTCCAAAGGACATCGCATTATATCAGTTGGTACAACTTCAACACGTACACTT
    GAAACAATTCGACGCGATCATGATAAATTTGTTGAAACGAGTGGCTGGACTAATATATTT
    ATTTATCCAGGATTTGATTTTAAAGCAATTGATGGCCAGATTACTAATTTTCATTTACCA
    AAATCAACATTAGTTATGCTAGTATCAGCGTTTAGTAGTCGTGAAAATGTTCTGAATGCT
    TATAAAACGGCAGTAAATTTAGAATATAGATTCTTTAGTTTTGGCGATGCAATGTTAATT
    ATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    840
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    960
    1020
    1026

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS08645 [old locus tag: SACOL1695 ]
  • symbol: SACOL_RS08645
  • description: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA
  • length: 341
  • theoretical pI: 5.29781
  • theoretical MW: 38968.3
  • GRAVY: -0.343988

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.4.99.17?  ExPASy
    S-adenosylmethionine:tRNA ribosyltransferase-isomerase S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine34 in tRNA = L-methionine + adenine + epoxyqueuosine34 in tRNA
    EC 5.-.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification S-adenosylmethionine:tRNA ribosyltransferase-isomerase (TIGR00113; EC 2.4.99.17; HMM-score: 476.2)
  • TheSEED: see SACOL1695
  • PFAM:
    no clan defined Queuosine_synth; Queuosine biosynthesis protein (PF02547; HMM-score: 469)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9926
    • Cytoplasmic Membrane Score: 0.0007
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0067
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003628
    • TAT(Tat/SPI): 0.000381
    • LIPO(Sec/SPII): 0.000497
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDDPDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSFGDAMLII

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1695
  • protein localization: see SACOL1695
  • quantitative data / protein copy number per cell: see SACOL1695
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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