From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2632 [new locus tag: SACOL_RS13775 ]
  • pan locus tag?: SAUPAN006318000
  • symbol: cudT
  • pan gene symbol?: cudT
  • synonym:
  • product: BCCT family osmoprotectant transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2632 [new locus tag: SACOL_RS13775 ]
  • symbol: cudT
  • product: BCCT family osmoprotectant transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2691407..2693029
  • length: 1623
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    TTGAAAAAAGAGAAAGTTATGGACTGGACGACCTTTATAGGGACAGTAGCTGTACTTCTT
    TTTGCAGTTATACCTATGATGGCTTTTCCAAAAGCAAGTGAAGATATCATCACTGGTATT
    AATAGTGCCATTTCTGATTCAATTGGTTCGATATATTTATTTATGGGGCTGGCGATTTTT
    TGCTTTGTAATGTATATCGCATTTGGTAAATATGGTAATGTCACACTTGGTAAAGCAAGT
    GATAAACCAGAATTTAATACATTTACATGGGCGGCAATGCTGTTTTGTGCAGGCATAGGC
    TCTGATATTTTATACTGGGGCGTTATTGAATGGGCTTTTTACTATCAAGTTCCACCAAAT
    GGCGCGAAAAGTATGAGTGATGAAGCACTCCAATATGCGACGCAATATGGTATGTTCCAC
    TGGGGGCCAATTGCTTGGGCTATTTATGTTCTACCAGCATTACCAATTGGTTATTTAGTA
    TTTGTTAAAAAACAACCGGTGTATAAAATTAGTCAAGCTTGTCGTCCGATTTTAAAAGGT
    CAAACAGATAAATTTGTAGGTAAAGTTGTAGATATCTTATTTATCTTTGGATTGCTAGGT
    GGTGCGGCAACATCACTAGCGTTAGGTGTGCCATTAATTTCTGCAGGCATAGAAAGATTA
    ACTGGTTTAGATGGTAAAAATATGATTTTACGTTCGGCCATTTTATTAACAATCACGGTT
    ATATTTGCCATTAGTTCATATACAGGATTGAAAAAAGGTATTCAAAAGTTAAGTGATATC
    AACGTTTGGCTATCCTTTGTACTTTTAGCCTTTATATTTATTATTGGACCGACTGTTTTT
    ATTATGGAAACGACAGTGACAGGGTTCGGAAATATGTTGAGAGATTTCTTTCATATGGCA
    ACATGGTTAGAACCATTCGGTGGTATTAAAGGTCGAAAAGAAACGAATTTCCCACAAGAC
    TGGACAATATTCTACTGGTCATGGTGGTTAGTATATGCGCCATTTATCGGTTTATTTATC
    GCTAGAATTTCAAAAGGTCGACGCCTTAAAGAAGTCGTGCTAGGAACAATTATTTATGGA
    ACGCTTGGATGCGTATTATTCTTTGGTATTTTTGGTAACTATGCTGTGTATTTACAAATT
    TCTGGACAGTTTAATGTAACACAATATTTAAATACACATGGTACAGAGGCAACCATTATT
    GAAGTGGTGCATCATTTACCATTCCCATCATTGATGATTGTACTATTCTTAGTATCTGCT
    TTCTTATTCTTAGCAACAACATTTGATTCGGGTTCATATATTTTAGCGGCAGCATCTCAG
    AAAAAAGTGGTAGGCGAACCATTACGTGCCAATCGTTTATTCTGGGCATTTGCATTGTGC
    TTATTGCCATTTTCATTGATGCTAGTTGGTGGTGAACGTGCATTAGAAGTATTGAAAACT
    GCTTCAATACTGGCAAGTGTGCCATTAATTGTTATTTTTATTTTCATGATGATATCATTT
    TTAATCATTTTAGGGCGCGATAGAATTAAACTTGAAACGCGTGCTGAAAAATTAAAAGAA
    GTTGAACGTCGTTCATTGCGAATCGTTCAAGTATCAGAAGAAGAACAAGACGATAATTTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1623

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2632 [new locus tag: SACOL_RS13775 ]
  • symbol: CudT
  • description: BCCT family osmoprotectant transporter
  • length: 540
  • theoretical pI: 9.09649
  • theoretical MW: 60254
  • GRAVY: 0.619259

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Other transporter, betaine/carnitine/choline transporter (BCCT) family (TIGR00842; HMM-score: 460.9)
  • TheSEED  :
    • Betaine/carnitine/choline transporter (BCCT) family
    Stress Response Osmotic stress Choline and Betaine Uptake and Betaine Biosynthesis  High-affinity choline uptake protein BetT
  • ⊞PFAM:
    APC (CL0062) BCCT; BCCT, betaine/carnitine/choline family transporter (PF02028; HMM-score: 507.5)
    and 2 more
    no clan defined AtTam38; AtTam38 family (PF25114; HMM-score: 15.2)
    PH (CL0266) DUF3093; Protein of unknown function (DUF3093) (PF11292; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.242236
    • TAT(Tat/SPI): 0.005112
    • LIPO(Sec/SPII): 0.056956
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAISDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSDILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLVFVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIERLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIGPTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAPFIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNTHGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRANRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDRIKLETRAEKLKEVERRSLRIVQVSEEEQDDNL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: ArgR/AhrC (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL2632&oldid=95640"