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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03385 [old locus tag: SA0588 ]
  • pan locus tag?: SAUPAN002508000
  • symbol: SA_RS03385
  • pan gene symbol?: mntB
  • synonym:
  • product: metal ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03385 [old locus tag: SA0588 ]
  • symbol: SA_RS03385
  • product: metal ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 681665..682501
  • length: 837
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (681665..682501) NCBI
  • BioCyc: SA_RS03385 BioCyc
  • MicrobesOnline: see SA0588

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTTAGAGTTTGTCGAACATTTATTTACATATCAATTCTTGAATCGAGCATTGATAACT
    TCAATTATTGTAGGGATAGTTTGTGGTACAGTTGGTAGTTTAATTGTATTACGTGGTCTT
    TCATTAATGGGAGATGCAATGAGTCACGCAGTATTACCTGGTGTTGCCCTATCATTCTTA
    TTTGGTATTCCAATGTTTGTAGGCGCACTTATAACTGGTATGATCGCAAGTATTTTTATC
    GGTTATATCACATCTAGTAGTAAAACGAAACCTGACGCCGCTATTGGAATTAGTTTTACC
    GCTTTCTTAGCTTCGGGGATTATTATTATTAGCTTAATAAACACTACGACAGATTTGTAC
    CATATTCTGTTTGGTAATTTACTGGCAATCACAAATAGTGCATTTTTAACAACTATTGTG
    ATTGGTTCAATCGTCCTTATTCTAATCATTATTTTCTATCGTCCATTAATGATTTCTACA
    TTCGATCCAACGTTTAGTAGAATGAGTGGTCTAAATACGACGTTATTACATTACTTTGTG
    ATGTTGTTACTCTCATTAGTAACAGTAGCAAGTATTCAAACGGTAGGTATTATTCTTGTA
    GTTGCATTATTAATTACACCTGCATCTACAGCATTTTTAATTAGTAAAAAACTATACTCA
    ATGATGATTATAGCAAGTTTAATAAGTGTCATAAGTTCAATTGTCGGACTTTATTACAGC
    TATATTTACAACATTCCTAGTGGTGCGACAATCGTACTTTGTACGTTTGTAATTTATATC
    ATCACATTATTTTTCACAAAATTTACGAATAGAAAGAAACGAGGAAGTTTAACATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    837

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03385 [old locus tag: SA0588 ]
  • symbol: SA_RS03385
  • description: metal ABC transporter permease
  • length: 278
  • theoretical pI: 9.77742
  • theoretical MW: 30104.2
  • GRAVY: 1.25108

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, permease subunit (TIGR03770; HMM-score: 182.1)
  • TheSEED: see SA0588
  • ⊞PFAM:
    Membrane_trans (CL0142) ABC-3; ABC 3 transport family (PF00950; HMM-score: 260.9)
    and 4 more
    FecCD; FecCD transport family (PF01032; HMM-score: 28)
    no clan defined Cation_ATPase_C; Cation transporting ATPase, C-terminus (PF00689; HMM-score: 15.2)
    4HB_MCP (CL0457) 4HB_MCP_1; Four helix bundle sensory module for signal transduction (PF12729; HMM-score: 10.4)
    no clan defined Dsc3_C; DSC E3 ubiquitin ligase complex subunit 3, C-terminal domain (PF13373; HMM-score: 8.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007622
    • TAT(Tat/SPI): 0.001273
    • LIPO(Sec/SPII): 0.024432
  • predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

  • GI: 446811996 NCBI
  • RefSeq: WP_000889252 NCBI
  • UniProt: see SA0588

⊟Protein sequence[edit | edit source]

  • MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMGDAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAFLASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMISTFDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKLYSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGSLT

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MntR* see SA0588

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS03385&oldid=63292"
  • This page was last edited on 10 March 2016, at 16:54.
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