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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00636
  • pan locus tag?: SAUPAN002508000
  • symbol: SAOUHSC_00636
  • pan gene symbol?: mntB
  • synonym:
  • product: iron (chelated) ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00636
  • symbol: SAOUHSC_00636
  • product: iron (chelated) ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 625507..626343
  • length: 837
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTTAGAGTTTGTCGAACATTTATTTACATATCAATTCTTGAATCGAGCATTGATAACT
    TCAATTATTGTAGGGATAGTTTGTGGTACAGTTGGTAGTTTAATTGTATTACGTGGTCTT
    TCATTAATGGGAGATGCAATGAGTCACGCAGTATTACCTGGTGTTGCCCTATCATTCTTA
    TTTGGTATTCCAATGTTTGTAGGCGCACTTATAACTGGTATGATCGCAAGTATTTTTATC
    GGTTATATCACATCTAGTAGTAAAACGAAACCTGACGCCGCTATTGGAATTAGTTTTACC
    GCTTTCTTAGCTTCGGGGATTATTATTATTAGCTTAATAAACACTACGACAGATTTGTAC
    CATATTCTGTTTGGTAATTTACTGGCAATCACAAATAGTGCATTTTTAACAACTATTGTG
    ATTGGTTCAATCGTCCTTATTCTAATCATTATTTTCTATCGTCCATTAATGATTTCTACA
    TTCGATCCAACGTTTAGTAGAATGAGTGGTCTAAATACGACGTTATTACATTACTTTGTG
    ATGTTGTTACTCTCATTAGTAACAGTAGCAAGTATTCAAACGGTAGGTATTATTCTTGTA
    GTTGCATTATTAATTACACCTGCATCTACAGCATTTTTAATTAGTAAAAAACTATACTCA
    ATGATGATTATAGCAAGTTTAATAAGTGTCATAAGTTCAATTGTCGGACTTTATTACAGC
    TATATTTACAACATTCCTAGTGGTGCGACAATCGTACTTTGTACGTTTGTAATTTATATC
    ATCACATTATTTTTCACAAAATTTACGAATAGAAAGAAACGAGGAAGTTTAACATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    837

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00636
  • symbol: SAOUHSC_00636
  • description: iron (chelated) ABC transporter permease
  • length: 278
  • theoretical pI: 9.77742
  • theoretical MW: 30104.2
  • GRAVY: 1.25108

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, permease subunit (TIGR03770; HMM-score: 182.1)
  • TheSEED  :
    • Manganese/zinc ABC transporter (EC 7.2.2.5), permease protein ScaB
  • PFAM:
    Membrane_trans (CL0142) ABC-3; ABC 3 transport family (PF00950; HMM-score: 260.9)
    and 4 more
    FecCD; FecCD transport family (PF01032; HMM-score: 28)
    no clan defined Cation_ATPase_C; Cation transporting ATPase, C-terminus (PF00689; HMM-score: 15.2)
    4HB_MCP (CL0457) 4HB_MCP_1; Four helix bundle sensory module for signal transduction (PF12729; HMM-score: 10.4)
    no clan defined Dsc3_C; DSC E3 ubiquitin ligase complex subunit 3, C-terminal domain (PF13373; HMM-score: 8.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007622
    • TAT(Tat/SPI): 0.001273
    • LIPO(Sec/SPII): 0.024432
  • predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMGDAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAFLASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMISTFDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKLYSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGSLT

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MntR* (repression) regulon
    MntR*(TF)important in Manganese homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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