⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS05865 [old locus tag: SACOL1148 ]
pan locus tag^?: SAUPAN003374000
symbol: SACOL_RS05865
pan gene symbol^?: pheS
• synonym:
• product: phenylalanine--tRNA ligase subunit alpha

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS05865 [old locus tag: SACOL1148 ]
• symbol: SACOL_RS05865
• product: phenylalanine--tRNA ligase subunit alpha
• replicon: chromosome
• strand: +
• coordinates: 1155545..1156603
• length: 1059
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1155545..1156603) NCBI
• BioCyc: SACOL_RS05865 BioCyc
• MicrobesOnline: see SACOL1148

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  1021
  
  ATGTCTGAACAACAAACAATGTCAGAGTTAAAACAACAAGCGCTTGTAGATATTAATGAA
  GCAAATGATGAACGTGCACTGCAAGAAGTTAAAGTGAAATACTTAGGTAAAAAAGGGTCA
  GTTAGCGGACTAATGAAATTGATGAAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGT
  CAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATG
  TTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCA
  GGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAA
  GACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGAACAAGATCAT
  TATAACTTCGAAATGCTGAATTTACCTAAATCACACCCTGCACGTGATATGCAAGATAGT
  TTCTATATTACGGATGAAATTTTATTACGTACGCATACATCACCAGTGCAGGCACGTACG
  ATGGAATCACGTCATGGTCAAGGTCCAGTTAAAATTATTTGCCCTGGTAAAGTGTATCGT
  CGTGACTCTGATGATGCGACACATAGTCATCAATTTACACAAATCGAAGGATTAGTTGTT
  GATAAAAACGTTAAAATGAGTGATTTGAAAGGTACTTTAGAATTGTTAGCTAAGAAATTA
  TTTGGTGCTGATCGTGAAATTCGTTTACGTCCAAGTTACTTCCCATTCACTGAACCTTCT
  GTAGAAGTTGATGTGTCATGTTTTAAATGTAAAGGAAAAGGTTGTAATGTGTGTAAACAC
  ACAGGATGGATTGAAATTTTAGGTGCTGGAATGGTACATCCTAATGTATTAGAAATGGCT
  GGTTTTGATTCTTCAGAGTACTCTGGATTTGCATTTGGTATGGGACCAGACCGTATTGCA
  ATGTTGAAATATGGTATAGAAGATATTCGTCATTTCTATACTAATGATGTGAGATTTTTA
  GATCAATTTAAAGCGGTAGAAGATAGAGGTGACATGTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS05865 [old locus tag: SACOL1148 ]
• symbol: SACOL_RS05865
• description: phenylalanine--tRNA ligase subunit alpha
• length: 352
• theoretical pI: 5.47515
• theoretical MW: 40106.4
• GRAVY: -0.540057

⊟Function

• ⊞⊞reaction:
  EC 6.1.1.20?  ExPASy
  Phenylalanine--tRNA ligase ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
• ⊞⊞TIGRFAM:
  Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 380.5)
  and 7 more
  Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase (TIGR00469; EC 6.1.1.20; HMM-score: 124.3)
  Genetic information processing Protein synthesis tRNA aminoacylation O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 48.9)
  Metabolism Amino acid biosynthesis Serine family O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 48.9)
  Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00471; EC 6.1.1.20; HMM-score: 18.9)
  Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 15.6)
  Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase, regulatory subunit (TIGR00443; HMM-score: 13.3)
  Genetic information processing Transcription Degradation of RNA ribonuclease, Rne/Rng family (TIGR00757; EC 3.1.4.-; HMM-score: 6.9)
• TheSEED: see SACOL1148
• ⊞⊞PFAM:
  tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 364.3)
  and 3 more
  tRNA_bind_arm (CL0298) Phe_tRNA-synt_N; Aminoacyl tRNA synthetase class II, N-terminal domain (PF02912; HMM-score: 83.7)
  tRNA_synt_II (CL0040) tRNA_synthFbeta; Phenylalanyl tRNA synthetase beta chain CLM domain (PF17759; HMM-score: 19.8)
  tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9974
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0026
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003802
  • TAT(Tat/SPI): 0.003535
  • LIPO(Sec/SPII): 0.000434
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445925704 NCBI
• RefSeq: WP_000003559 NCBI
• UniProt: see SACOL1148

⊟Protein sequence

• MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGLMKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGRHIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDSFYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGLVVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCNVCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYTNDVRFLDQFKAVEDRGDM

⊟Experimental data

• experimentally validated: see SACOL1148
• protein localization: see SACOL1148
• quantitative data / protein copy number per cell: see SACOL1148
• ⊟⊟interaction partners:

  SACOL_RS01040		formate acetyltransferase   (data from MRSA252)
  SACOL_RS03080		elongation factor Tu   (data from MRSA252)
  SACOL_RS04840		NADH dehydrogenase   (data from MRSA252)
  SACOL_RS05630		pyruvate dehydrogenase E1 component subunit alpha   (data from MRSA252)
  SACOL_RS05645		dihydrolipoyl dehydrogenase   (data from MRSA252)
  SACOL_RS05870		phenylalanine--tRNA ligase subunit beta   (data from MRSA252)
  SACOL_RS06505		30S ribosomal protein S2   (data from MRSA252)
  SACOL_RS07385		dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex   (data from MRSA252)
  SACOL_RS08865		aldehyde dehydrogenase   (data from MRSA252)
  SACOL_RS08905		isocitrate dehydrogenase (NADP(+))   (data from MRSA252)
  SACOL_RS08930		pyruvate kinase   (data from MRSA252)
  SACOL_RS09045		30S ribosomal protein S4   (data from MRSA252)
  SACOL_RS11615		30S ribosomal protein S9   (data from MRSA252)
  SACOL_RS11685		50S ribosomal protein L15   (data from MRSA252)
  SACOL_RS11695		30S ribosomal protein S5   (data from MRSA252)
  SACOL_RS11770		50S ribosomal protein L23   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: T-box(Phe) see SACOL1148

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1148

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications