From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0979 [new locus tag: SA_RS05550 ]
  • pan locus tag?: SAUPAN003362000
  • symbol: isdD
  • pan gene symbol?: isdD
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0979 [new locus tag: SA_RS05550 ]
  • symbol: isdD
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1108742..1109818
  • length: 1077
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGAAATGTTAAACAAATTGCTACAAAATCTATTATAGCTATTATTAGCTTATGTATA
    CTTACATATACAACAATGATTGGTAGCGTGTTGGCTGATGAGATAAAATATCCATCAGCC
    AAATTTAATCAACCTGAAGCAAAAGATAAAACAGAATTAACTACATCAATTTTTGATGAA
    AAGATAAAAGAGAATAAAGCGTTAGAGTTACTAATTTTTAATCAAGAAAATAAAAATGTA
    ACTGAGGAACAACAACTAGTTGACGAAAAGGCGCAATTGATTTCAGATATGACTGGTAAA
    ATTTACTTGCAAGTAAAGCTAAAAGGTCAAATAGATAAAGAACAACTTGTTTTTCAAAAT
    GACAAAAATGAAGAATTTCCTTTTGTTATAAAAGATGAAAAGGATGACACAATAGTAAGA
    ATTTTAATTGAACAGCATATGGATAAAATCAATATGCATGTTAAAACGTTGGCTGAAAAG
    AAAAATCTAGATAACAAAGAAATGGTGTATTCTATTCATTTTAAAGAGAAAAAAGTACAA
    CATGATGATGCAAAAGAAGTGCCTTCAAAACATCAAAATCAAGAAAATAATCAAGATCAG
    CTTAAAAAAGATATTGATGACAAAAAAGATAGTCAAAAATCAGATACTAAGGAAAGACGT
    ACTAGCCTTTTTACTGAAAAAGGATTAAATGATATTCCTGTACAAAAAGATAAAGTGCAA
    CAAGACAGTAATAAAAAGATTGAAAATGAGCGACCTAAAGCATCAGGTACATTAAAAGTT
    GAAAATAGCCCTCCAACAATAAAAAAGGTTGAAAATAATCACAAAGAGCAACCGAAACAT
    AAAGATGAAAAATCAAAAAAGGAAAAGAAAAAAGTAGTTGAAAAAGAAAAAGCGTTACCA
    GCTTTTAATAGAGATGATGATAGCAAGAATAGTAGTCAATTATCTAGTGATATTAAAGAA
    CTTGATGAACCAAATCATAAAAAGCAATATATGTTATTTGCAGCTGGCATTGTGTTAGCA
    ACTATTTTACTTATTTCGGCACATTTATACAGCAGAAAGAGAGGTAACCAAGTTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1077

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0979 [new locus tag: SA_RS05550 ]
  • symbol: IsdD
  • description: hypothetical protein
  • length: 358
  • theoretical pI: 9.39959
  • theoretical MW: 41393.9
  • GRAVY: -0.984358

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Heme transporter IsdDEF, membrane component IsdD
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme transporter IsdDEF, membrane component IsdD
  • PFAM:

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.7981
    • Cell wall & surface Score: 0.046
    • Extracellular Score: 0.1558
  • ⊞LocateP: C-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: SVLADEIK
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 32 aa
    • SP(Sec/SPI): 0.985108
    • TAT(Tat/SPI): 0.001277
    • LIPO(Sec/SPII): 0.001536
    • Cleavage Site: CS pos: 32-33. VLA-DE. Pr: 0.9724
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRNVKQIATKSIIAIISLCILTYTTMIGSVLADEIKYPSAKFNQPEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIYLQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEKKNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKERRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKEQPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFAAGIVLATILLISAHLYSRKRGNQV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: Fur* (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Michelle L Reniere, Eric P Skaar
    Staphylococcus aureus haem oxygenases are differentially regulated by iron and haem.
    Mol Microbiol: 2008, 69(5);1304-15
    [PubMed:18643935] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA0979&oldid=89008"