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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0620 [new locus tag: NWMN_RS03525 ]
  • pan locus tag?: SAUPAN002529000
  • symbol: NWMN_0620
  • pan gene symbol?: dhaL
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0620 [new locus tag: NWMN_RS03525 ]
  • symbol: NWMN_0620
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 702583..703167
  • length: 585
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAAGTGAATGATATGAAAGCACGTTTATTAAATTTAGAAGAAACGTTTAAAAAACAT
    GAATCTGAATTAACTGAATTAGATCGAGCAATTGGTGATGGTGACCACGGGGTTAACATG
    GTTCGTGGGTTTAGTAGTCTTAAAGACAAACTTGATGATAGCTCAATGCAATCATTGTTC
    AAATCAACTGGTATGGCATTGATGTCAAATGTTGGGGGTGCATCAGGACCACTGTATGGC
    TTTAGCTTTGTTAAAATGTCTGCAGTCACCAAAGATGATATGGATAATCAAGATTTCATT
    ACACTAATTCAGGCATTTGCCGAAGCGGTTGAATCACGTGGTAAAGTTACTTTAAATGAA
    AAGACAATGTATGATGTAGTAGCGCGAGCAGCAGAGAAGCTTAAAAATGGTGAAACTTTA
    ACATTCAATGATTTACAGCAATTAGCAGATAATACAAAAGATATGGTAGCAACGAAAGGT
    AGAGCTGCATATTTTGGAGAAGAATCAAAAGGTTATATTGATCCAGGTGCTCAAAGTATG
    GTTTATATTTTAAACGCTTTGATTGGAGATGAAGATAATGCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    585

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0620 [new locus tag: NWMN_RS03525 ]
  • symbol: NWMN_0620
  • description: hypothetical protein
  • length: 194
  • theoretical pI: 4.53188
  • theoretical MW: 21260.9
  • GRAVY: -0.384021

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    dihydroxyacetone kinase, L subunit (TIGR02365; EC 2.7.-.-; HMM-score: 240.1)
    and 2 more
    dihydroxyacetone kinase (TIGR02361; EC 2.7.1.29; HMM-score: 79.3)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS7 (TIGR01029; HMM-score: 13.5)
  • TheSEED  :
    • Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), ADP-binding subunit DhaL
    Carbohydrates Central carbohydrate metabolism Dihydroxyacetone kinases  Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), ADP-binding subunit DhaL
  • ⊞PFAM:
    no clan defined Dak2; DAK2 domain (PF02734; HMM-score: 153.4)
    and 3 more
    ATPgrasp_N; ATP-grasp N-terminal domain (PF18130; HMM-score: 13.7)
    Phage_TACs (CL0567) Phage_TAC_12; Phage tail assembly chaperone protein, TAC (PF12363; HMM-score: 12.9)
    no clan defined Cluap1; Clusterin-associated protein-1 (PF10234; HMM-score: 9.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9636
    • Cytoplasmic Membrane Score: 0.0075
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0286
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015718
    • TAT(Tat/SPI): 0.000729
    • LIPO(Sec/SPII): 0.000894
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGFSSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITLIQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKGRAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: CcpA regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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