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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000618
  • pan locus tag?: SAUPAN002529000
  • symbol: dhaL
  • pan gene symbol?: dhaL
  • synonym:
  • product: dihydroxyacetone kinase subunit DhaL

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000618
  • symbol: dhaL
  • product: dihydroxyacetone kinase subunit DhaL
  • replicon: chromosome
  • strand: +
  • coordinates: 654056..654640
  • length: 585
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAAGTGAATGATATGAAAGCACGTTTATTAAATTTAGAAGAAACGTTTAAAAAACAT
    GAATCTGAATTAACTGAATTAGATCGAGCAATTGGTGATGGTGACCACGGGGTTAACATG
    GTTCGTGGGTTTAGTAGTCTTAAAGACAAACTTGATGATAGCTCAATGCAATCATTGTTC
    AAATCGACTGGTATGGCATTGATGTCAAATGTTGGGGGTGCATCAGGACCACTGTATGGC
    TTTAGCTTTGTTAAAATGTCTGCAGTCACCAAAGATGATATGGATAATCAAGATTTCATT
    ACACTAATTCAGGCATTTGCCGAAGCGGTTGAATCACGTGGTAAAGTTACTTTAAATGAA
    AAGACAATGTATGATGTAGTAGCGCGAGCAGCAGAGAAGCTTGAAAATGGTGAAACTTTA
    ACATTCAATGATTTACAGCAATTAGCAGATAATACAAAAGATATGGTAGCAACGAAAGGT
    AGAGCTGCATATTTTGGAGAAGAATCAAAAGGTTATATTGATCCAGGTGCTCAAAGTATG
    GTTTATATTTTAAACGCTTTGATTGGAGATGAAGATAATGCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    585

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000618
  • symbol: DhaL
  • description: dihydroxyacetone kinase subunit DhaL
  • length: 194
  • theoretical pI: 4.40795
  • theoretical MW: 21261.8
  • GRAVY: -0.381959

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.121?  ExPASy
    Phosphoenolpyruvate--glycerone phosphotransferase Phosphoenolpyruvate + glycerone = pyruvate + glycerone phosphate
  • TIGRFAM:
    dihydroxyacetone kinase, L subunit (TIGR02365; EC 2.7.-.-; HMM-score: 239.9)
    and 2 more
    dihydroxyacetone kinase (TIGR02361; EC 2.7.1.29; HMM-score: 79.3)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS7 (TIGR01029; HMM-score: 13.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Dak2; DAK2 domain (PF02734; HMM-score: 153.5)
    and 1 more
    ATPgrasp_N; ATP-grasp N-terminal domain (PF18130; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9747
    • Cytoplasmic Membrane Score: 0.0075
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.0172
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015718
    • TAT(Tat/SPI): 0.000729
    • LIPO(Sec/SPII): 0.000894
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGFSSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITLIQAFAEAVESRGKVTLNEKTMYDVVARAAEKLENGETLTFNDLQQLADNTKDMVATKGRAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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