⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_0399 [new locus tag: NWMN_RS02260 ]
pan locus tag^?: SAUPAN002122000
symbol: hsdS
pan gene symbol^?: hsdS1
• synonym:
• product: type I restriction modification system, site specificity determination subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0399 [new locus tag: NWMN_RS02260 ]
• symbol: hsdS
• product: type I restriction modification system, site specificity determination subunit
• replicon: chromosome
• strand: +
• coordinates: 448640..449851
• length: 1212
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332016 NCBI
• RefSeq: YP_001331433 NCBI
• BioCyc:
• MicrobesOnline: 3705930 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  481
  541
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  661
  721
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  841
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  1021
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  1141
  1201
  
  ATGAGTAATACACAAAAGAAAAATGTGCCAGAATTGAGGTTCCCAGGGTTTGAAGGCGAA
  TGGGAAGAGAAGCAGTTAGGGGATCTTACAGATAGAGTAATTAGGAAAAATAAAAACTTA
  GAATCGAAAAAGCCTTTAACAATATCCGGACAGTTAGGTTTAATTGATCAAACAGAATAT
  TTTAGTAAATCAGTTTCGTCGAAAAATCTAGAAAATTATACACTAATAAAGAATGGAGAA
  TTCGCGTATAACAAAAGTTATTCTAATGGATACCCATTAGGGGCTATTAAAAGATTAACT
  AGATATGATAGTGGTGTATTGTCCTCTTTGTATATTTGTTTTTCTATTAAAAGTGAAATG
  TCTAAAGACTTCATGGAAGCATATTTTGATTCGACACACTGGTATAGAGAAGTTTCTGGA
  ATTGCAGTTGAGGGTGCAAGAAATCACGGATTATTAAATGTTTCTGTGAATGATTTTTTT
  ACTATTCTAATTAAATATCCAAGTTTAGAAGAACAGCAAAAAATAGGCAAGTTCTTCAGC
  AAACTCGACCGACAAATTGAATTAGAAGAACAAAAGCTTGAATTACTTCAACAACAGAAA
  AAAGGCTATATGCAGAAAATTTTCTCACAGGAACTGCGATTCAAAGATGAGAATGGTGAA
  GATTATCCAGATTGGGAAAATAGCAAAATAGAAAAATATTTAAAAGAGAGAAACGAACGT
  TCTGACAAAGGGCAAATGCTTTCAGTAACTATAAATAGTGGCATTATAAAATTTAGTGAA
  TTGGATAGAAAAGATAATTCAAGTAAAGATAAAAGTAATTATAAAGTAGTTAGGAAAAAT
  GATATTGCATATAATTCTATGAGAATGTGGCAAGGGGCTAGTGGTAAATCAAATTATAAT
  GGGATTGTTAGCCCTGCATATACTGTGCTTTATCCAACACAAAATACTAGCTCATTATTT
  ATTGGATATAAGTTTAAAACACATAGAATGATTCATAAATTTAAAATTAATTCACAAGGA
  TTAACATCAGATACATGGAACTTAAAATATAAACAATTAAAAAATATAAATATAGATATA
  CCTGTATTGGAGGAACAAGAAAAGATAGGTGATTTCTTTAAAAAAATGGATATATTGATA
  AGTAAACAGAAAATGAAAATTGAAATATTAGAAAAAGAGAAACAATCCTTTTTACAAAAA
  ATGTTCTTATAA
  60
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  1212
  

⊟Protein

⊟General

• locus tag: NWMN_0399 [new locus tag: NWMN_RS02260 ]
• symbol: HsdS
• description: type I restriction modification system, site specificity determination subunit
• length: 403
• theoretical pI: 9.74077
• theoretical MW: 47135.4
• GRAVY: -0.74665

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Cell division septum site-determining protein MinD (TIGR01968; HMM-score: 12.2)
• ⊞⊞TheSEED  :
  • Type I restriction-modification system, specificity subunit S
  DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System  Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)
  and 1 more
  DNA Metabolism DNA Metabolism - no subcategory Type I Restriction-Modification  Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)
• PFAM:
  TRD (CL0477) Methylase_S; Type I restriction modification DNA specificity domain (PF01420; HMM-score: 54.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.5779
  • Cytoplasmic Membrane Score: 0.0328
  • Cell wall & surface Score: 0.0075
  • Extracellular Score: 0.3818
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00574
  • TAT(Tat/SPI): 0.000973
  • LIPO(Sec/SPII): 0.00056
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220611 NCBI
• RefSeq: YP_001331433 NCBI
• UniProt: A0A0H3K704 UniProt

⊟Protein sequence

• MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQTEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFSIKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIEKYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRMWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWNLKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hsdM > hsdS

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications