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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12530 [old locus tag: SA2184 ]
  • pan locus tag?: SAUPAN005927000
  • symbol: SA_RS12530
  • pan gene symbol?: narH
  • synonym:
  • product: nitrate reductase subunit beta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12530 [old locus tag: SA2184 ]
  • symbol: SA_RS12530
  • product: nitrate reductase subunit beta
  • replicon: chromosome
  • strand: -
  • coordinates: 2451164..2452723
  • length: 1560
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2451164..2452723) NCBI
  • BioCyc: SA_RS12530 BioCyc
  • MicrobesOnline: see SA2184

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    TTGAAGATTAAAGCGCAAGTTGCGATGGTATTAAATTTAGATAAATGCATAGGATGCCAT
    ACGTGTAGTGTGACATGTAAAAACACTTGGACAAATCGTCCAGGTGCTGAGTACATGTGG
    TTCAATAACGTAGAAACGAAGCCAGGTGTAGGGTATCCGAAACGTTGGGAAGACCAAGAA
    CACTACAAAGGTGGTTGGGTACTAAATCGTAAAGGGAAACTTGAATTAAAATCTGGAAGT
    AGAATTTCAAAAATTGCTTTAGGTAAAATTTTTTATAACCCAGATATGCCATTAATAAAA
    GATTATTATGAACCATGGAACTATAATTATGAACATTTAACAACTGCGAAATCAGGGAAG
    CATTCGCCAGTTGCTAGAGCGTATTCAGAAATTACAGGGGATAACATTGAAATTGAATGG
    GGACCTAACTGGGAAGATGACTTAGCAGGTGGTCATGTTACAGGCCCAAAAGATCCTAAC
    ATCCAAAAAATAGAAGAAGATATTAAATTCCAATTTGACGAAACTTTTATGATGTATTTA
    CCACGTTTATGTGAACATTGCTTGAATCCAAGTTGCGTTGCATCGTGCCCTTCAGGTGCT
    ATGTATAAACGTGATGAAGATGGCATTGTATTAGTTGATCAAGACGCATGTCGTGGATGG
    CGTTACTGTATGACAGGTTGTCCATATAAGAAAGTTTACTTCAATTGGAAAACGAACAAA
    GCTGAAAAATGTACTTTCTGTTTTCCGAGAATCGAGGCGGGTATGCCTACGGTATGCTCT
    GAAACTTGTACAGGACGTATGCGTTACTTAGGTGTTTTATTATATGATGCAGATCGAGTT
    CATGAAGCGGCTTCAGCAGTAGACGAGAAAGGTTTATACGAAAAACAATTAGATATATTC
    TTAAATCCATTTGACGAAGAAGTCATTGCTCAAGCTGAAAAAGATGGTATTGGTTATGAT
    TGGATTGAAGCTGCACAAAACTCTCCAATTTATAAGTTAGCAATTGAATATAAACTTGCA
    TTTCCGTTACATCCTGAATTTAGAACGATGCCGATGGTTTGGTATTGCCCACCACTTAGC
    CCAATTATGAGCTATTTTGAAGGTAAAAATACAACTCAAAATCCAGATGCGATTTTCCCA
    GCCATTGAAGAAATGCGTTTACCTATTGAATACTTAGCTAATATTTTCACTGCAGGAGAT
    ACAGAGCCAGTAAAAGGAGCCCTACAACGTATGGCAATGATGAGAAGTTATATGAGATCT
    CAAGTTACTCAACAGCCATTTGACACGTCTCGATTAGAGCGATTAGGTATAACCGAGCGC
    CAAACTAAAGACATGTATCGTTTATTAGGACTAGCTAAATATGAAGATCGATTTGTTATT
    CCAACATCACACAAAGAAACCTATTTAGATACGTATCACGCACAAGGTAGTACAGGATAC
    AATTACGGCGGCGAGCATTTTGGAGATAACTGTGAAGGCTGTGGCGTTGCAGTAGGTTCA
    GGGAAAACTGGTCAAGAAATTTATAATGAGAATTTCTATGGAGGGATTTTCCGTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS12530 [old locus tag: SA2184 ]
  • symbol: SA_RS12530
  • description: nitrate reductase subunit beta
  • length: 519
  • theoretical pI: 5.68879
  • theoretical MW: 59372.2
  • GRAVY: -0.530636

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Energy metabolism Anaerobic nitrate reductase, beta subunit (TIGR01660; EC 1.7.99.4; HMM-score: 837.9)
    and 21 more
    Metabolism Energy metabolism Anaerobic DMSO reductase family type II enzyme, iron-sulfur subunit (TIGR03478; HMM-score: 288.7)
    Metabolism Energy metabolism Electron transport DMSO reductase family type II enzyme, iron-sulfur subunit (TIGR03478; HMM-score: 288.7)
    dimethylsulfoxide reductase, chain B (TIGR02951; HMM-score: 107)
    Metabolism Energy metabolism Electron transport cytochrome c nitrite reductase, Fe-S protein (TIGR03149; EC 1.7.2.2; HMM-score: 86.6)
    Metabolism Energy metabolism Anaerobic formate dehydrogenase, beta subunit (TIGR01582; EC 1.2.1.2; HMM-score: 71.9)
    Metabolism Energy metabolism Electron transport formate dehydrogenase, beta subunit (TIGR01582; EC 1.2.1.2; HMM-score: 71.9)
    [FeFe] hydrogenase, group B1/B3 (TIGR04105; EC 1.12.-.-; HMM-score: 22.6)
    Unknown function General archaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 22.3)
    2-oxoacid:acceptor oxidoreductase, delta subunit, pyruvate/2-ketoisovalerate family (TIGR02179; HMM-score: 16.2)
    Genetic information processing Protein fate Protein modification and repair glycine radical enzyme activase, YjjW family (TIGR04041; EC 1.97.1.-; HMM-score: 15.7)
    Metabolism Central intermediary metabolism Nitrogen fixation ferredoxin III, nif-specific (TIGR02936; HMM-score: 14.9)
    methylamine methyltransferase corrinoid protein reductive activase (TIGR04270; HMM-score: 14.8)
    Metabolism Energy metabolism Amino acids and amines choline TMA-lyase-activating enzyme (TIGR04395; EC 1.97.-.-; HMM-score: 14.5)
    glycyl-radical enzyme activating protein (TIGR02494; EC 1.97.1.-; HMM-score: 12.2)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfite reductase, dissimilatory-type beta subunit (TIGR02066; EC 1.8.99.3; HMM-score: 10.9)
    coenzyme F420 hydrogenase, subunit gamma (TIGR03294; EC 1.12.98.1; HMM-score: 9.2)
    [FeFe] hydrogenase, group A (TIGR02512; EC 1.12.-.-; HMM-score: 8.1)
    Metabolism Energy metabolism Electron transport electron transport complex, RnfABCDGE type, B subunit (TIGR01944; HMM-score: 5.8)
    Metabolism Energy metabolism Methanogenesis putative methanogenesis marker 16 metalloprotein (TIGR03287; HMM-score: 4.3)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfite reductase, subunit C (TIGR02912; EC 1.8.-.-; HMM-score: 4)
    Metabolism Energy metabolism Other benzoyl-CoA oxygenase/reductase, BoxA protein (TIGR03224; EC 1.14.12.21; HMM-score: 3.9)
  • TheSEED: see SA2184
  • ⊞PFAM:
    4Fe-4S (CL0344) Fer4_11; 4Fe-4S dicluster domain (PF13247; HMM-score: 125.1)
    no clan defined Nitr_red_bet_C; Respiratory nitrate reductase beta C-terminal (PF14711; HMM-score: 101.5)
    and 11 more
    4Fe-4S (CL0344) Fer4_10; 4Fe-4S dicluster domain (PF13237; HMM-score: 37.5)
    Fer4_7; 4Fe-4S dicluster domain (PF12838; HMM-score: 29.1)
    Fer4_2; 4Fe-4S binding domain (PF12797; HMM-score: 19.9)
    Fer4_9; 4Fe-4S dicluster domain (PF13187; HMM-score: 17.7)
    Fer4_6; 4Fe-4S binding domain (PF12837; HMM-score: 17.1)
    Fer4_13; 4Fe-4S single cluster domain of Ferredoxin I (PF13370; HMM-score: 16.4)
    Fer4; 4Fe-4S binding domain (PF00037; HMM-score: 15)
    SUKH (CL0526) SUKH_5; SMI1-KNR4 cell-wall (PF14567; HMM-score: 12.6)
    4Fe-4S (CL0344) Fer4_8; 4Fe-4S dicluster domain (PF13183; HMM-score: 11.5)
    Fer4_21; 4Fe-4S dicluster domain (PF14697; HMM-score: 10.6)
    Fer4_17; 4Fe-4S dicluster domain (PF13534; HMM-score: 9.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.1428
    • Cytoplasmic Membrane Score: 0.4808
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.3762
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.193248
    • TAT(Tat/SPI): 0.002364
    • LIPO(Sec/SPII): 0.257463
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKGLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: NreC*, Rex* see SA2184

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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