PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1728 [new locus tag: SA_RS09920 ]
pan locus tag^?: SAUPAN004945000
symbol: nadE
pan gene symbol^?: nadE
• synonym:
• product: NAD synthetase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1728 [new locus tag: SA_RS09920 ]
• symbol: nadE
• product: NAD synthetase
• replicon: chromosome
• strand: -
• coordinates: 1978963..1979784
• length: 822
• essential: yes DEG other strains

⊟Accession numbers

• Gene ID: 1124612 NCBI
• RefSeq: NP_375019 NCBI
• BioCyc: see SA_RS09920
• MicrobesOnline: 104045 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAGTAAATTACAAGACGTTATTGTACAAGAAATGAAAGTGAAAAAGCGTATCGATAGT
  GCTGAAGAAATTATGGAATTAAAGCAATTTATAAAAAATTATGTACAATCACATTCATTT
  ATAAAATCTTTAGTGTTAGGTATTTCAGGAGGACAAGATTCTACATTAGTTGGAAAACTA
  GTACAAATGTCTGTTAACGAATTACGTGAAGAAGGCATTGATTGTACGTTTATTGCAGTT
  AAATTACCTTATGGAGTTCAAAAAGATGCTGATGAAGTTGACCAAGCTTTGCGATTCATT
  GAACCAGATGAAATAGTAACAGTCAATATTAAGCCTGCAGTTGATCAAAGTGTGCAATCA
  TTAAAAGAAGCTGGTATTGTTCTTACAGATTTCCAAAAAGGAAATGAAAAAGCGCGTGAA
  CGTATGAAAGTACAATTTTCAATTGCTTCAAACCGACAAGGTATTGTAGTAGGAACAGAT
  CATTCAGCTGAAAATATAACTGGGTTTTATACGAAGTACGGTGATGGTGCTGCAGATATC
  GCACCTATATTTGGTTTGAATAAACGACAAGGTCGTCAATTATTAGCGTATCTTGGTGCG
  CCAAAGGAATTATATGAAAAAACGCCAACTGCTGATTTAGAAGATGATAAACCACAGCTT
  CCAGATGAAGATGCATTAGGTGTAACTTATGAGGCGATTGATAATTATTTAGAAGGTAAG
  CCAGTTACGCCAGAAGAACAAAAAGTAATTGAAAATCATTATATACGAAATGCACACAAA
  CGTGAACTTGCATATACAAGATACACGTGGCCAAAATCCTAA
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  822
  

⊟Protein

⊟General

• locus tag: SA1728 [new locus tag: SA_RS09920 ]
• symbol: NadE
• description: NAD synthetase
• length: 273
• theoretical pI: 4.99962
• theoretical MW: 30682.6
• GRAVY: -0.477656

⊟Function

• ⊞reaction:
  EC 6.3.1.5?  ExPASy
  NAD⁺ synthase ATP + deamido-NAD⁺ + NH₃ = AMP + diphosphate + NAD⁺
• ⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 232.6)
  and 2 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 14.3)
  Genetic information processing DNA metabolism Restriction/modification putative sulfurtransferase DndC (TIGR03183; HMM-score: 11.4)
• TheSEED  :
  • NAD synthetase (EC 6.3.1.5)
  Cofactors, Vitamins, Prosthetic Groups, Pigments NAD and NADP NAD and NADP cofactor biosynthesis global  NAD synthetase (EC 6.3.1.5)
• ⊞PFAM:
  HUP (CL0039) NAD_synthase; NAD synthase (PF02540; HMM-score: 219.9)
  and 4 more
  ATP_bind_3; PP-loop family (PF01171; HMM-score: 13.2)
  GME (CL0197) PAD_porph; Porphyromonas-type peptidyl-arginine deiminase (PF04371; HMM-score: 12.5)
  no clan defined DUF3576; Domain of unknown function (DUF3576) (PF12100; HMM-score: 12.5)
  GlnB-like (CL0089) NRho; Rhomboid N-terminal domain (PF16733; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9869
  • Cytoplasmic Membrane Score: 0.0011
  • Cell wall & surface Score: 0.0034
  • Extracellular Score: 0.0085
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005328
  • TAT(Tat/SPI): 0.002313
  • LIPO(Sec/SPII): 0.000556
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927486 NCBI
• RefSeq: NP_375019 NCBI
• UniProt: P99150 UniProt

⊟Protein sequence

• MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEPDEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTDHSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: nadE < SA1729

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)