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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1893 [new locus tag: SAUSA300_RS10360 ]
  • pan locus tag?: SAUPAN004945000
  • symbol: nadE
  • pan gene symbol?: nadE
  • synonym:
  • product: NAD synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1893 [new locus tag: SAUSA300_RS10360 ]
  • symbol: nadE
  • product: NAD synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 2057924..2058745
  • length: 822
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAGTAAATTACAAGACGTTATTGTACAAGAAATGAAAGTGAAAAAGCGTATCGATAGT
    GCTGAAGAAATTATGGAATTAAAGCAATTTATAAAAAATTATGTACAATCACATTCATTT
    ATAAAATCTTTAGTGTTAGGTATTTCAGGAGGACAGGATTCTACATTAGTTGGAAAACTA
    GTACAAATGTCTGTTAACGAATTACGTGAAGAAGGCATTGATTGTACGTTTATTGCAGTT
    AAATTACCTTATGGAGTTCAAAAAGATGCTGATGAAGTTGAGCAAGCTTTGCGATTCATT
    GAACCAGATGAAATAGTAACAGTCAATATTAAGCCTGCAGTTGATCAAAGTGTGCAATCA
    TTAAAAGAAGCCGGTATTGTTCTTACAGATTTCCAAAAAGGAAATGAAAAAGCGCGTGAA
    CGTATGAAAGTACAATTTTCAATTGCTTCAAACCGACAAGGTATTGTAGTAGGAACAGAT
    CATTCAGCTGAAAATATAACTGGGTTTTATACGAAGTACGGTGATGGTGCTGCAGATATC
    GCACCTATATTTGGTTTGAATAAACGACAAGGTCGTCAATTATTAGCGTATCTTGGTGCG
    CCAAAGGAATTATATGAAAAAACGCCAACTGCTGATTTAGAAGATGATAAACCACAGCTT
    CCAGATGAAGATGCATTAGGTGTAACTTATGAGGCGATTGATAATTATTTAGAAGGTAAG
    CCAGTTACGCCAGAAGAACAAAAAGTAATTGAAAATCATTATATACGAAATGCACACAAA
    CGTGAACTTGCATATACAAGATACACGTGGCCAAAATCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    822

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1893 [new locus tag: SAUSA300_RS10360 ]
  • symbol: NadE
  • description: NAD synthetase
  • length: 273
  • theoretical pI: 5.00393
  • theoretical MW: 30696.6
  • GRAVY: -0.477656

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.1.5?  ExPASy
    NAD+ synthase ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 233.6)
    and 3 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 15.2)
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 11.9)
    Genetic information processing DNA metabolism Restriction/modification putative sulfurtransferase DndC (TIGR03183; HMM-score: 11.4)
  • TheSEED  :
    • NAD synthetase (EC 6.3.1.5)
    Cofactors, Vitamins, Prosthetic Groups, Pigments NAD and NADP NAD and NADP cofactor biosynthesis global  NAD synthetase (EC 6.3.1.5)
  • PFAM:
    HUP (CL0039) NAD_synthase; NAD synthase (PF02540; HMM-score: 226.6)
    and 3 more
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 14)
    no clan defined DUF3576; Domain of unknown function (DUF3576) (PF12100; HMM-score: 13.2)
    Nucleoporin_C; Non-repetitive/WGA-negative nucleoporin C-terminal (PF03177; HMM-score: 9.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005328
    • TAT(Tat/SPI): 0.002313
    • LIPO(Sec/SPII): 0.000556
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEPDEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTDHSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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