From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0315 [new locus tag: NWMN_RS01795 ]
  • pan locus tag?: SAUPAN001852000
  • symbol: NWMN_0315
  • pan gene symbol?: —
  • synonym:
  • product: NADH-dependent flavin oxidoreductase Oye family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0315 [new locus tag: NWMN_RS01795 ]
  • symbol: NWMN_0315
  • product: NADH-dependent flavin oxidoreductase Oye family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 365224..366423
  • length: 1200
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTACCGATACAAACCACTTTTACAATCAATCCACTTACCTAATGGCATCAAAATTTCG
    AATCGCTTTGTATTATCACCAATGACCGTAAACACATCAACAAAAGAAGGCTATATTACA
    AAAGAAGACTTGGCTTATGCTGCACGTCGCTCTAATTCAGCTGGAATGCAAGTCACCGGC
    GCTGCTTACATTGAACCTTATGGTCAATTGTTTGAATATGGCTTTAATATTGATCATGAT
    GCTTGTATCCCTGGATTAACTAACATGGCATCTACGATGAAACAACATGGTAGCCTCGCT
    ATTATCCAGCTAGCGCATGCTGGTCGTTTTTCAAATCAAGCCATTTTAAATTTTGGAAAG
    GTGTATGGACCAAGCCCTATGACATTGCATTCACCGATTGAACACGATGTTATCGCCATG
    TCTCATGAGAAAATCAATAGCATCATTCAGCAGTATCGCGATGCTACATTACGCGCGATT
    AAAGCAGGTTTTGATGGTGTGGAAATTTCGATTGCACAACGTTTGCTGATTCAAACATTT
    TTCTCAACTTTTTCGAATAAACGTACAGACCATTACGGTGCCGACTCATTAAAAAATCGT
    GCAAGATTATGTTTAGAAGTTATGCGTGCGGTACAAGAAGTGATTGATAAAGAAGCACCT
    GACAATTTTATTTTAGGATTTCGAGCAACGCCAGAAGAAACCAGAGGCAGTGATTTAGGA
    TATACCATTGACGAGTTCAACCAATTAATCAATTGGGTCATGGACGTTTCAAACATCCAA
    TACTTAGCAATCGCTAGCTGGGGTCGACATATTTATCAAAATACGTCTCGTACACCAGGT
    GATCATTTCGGTCGACCAGTTAACCAAATTGTCTATGAACATTTAGCAGGTCGTATCCCT
    TTAATTGCAAGTGGCGGGATTAATTCTCCAGAGTCTGCTTTAGATGCTTTACAACATGCA
    GATATGGTCGGCATGTCTTCGCCTTTTGTCACAGAACCTGACTTTGTGCATAAACTTGCT
    GAACAGCGACCACATGATATCAATCTTGAATTTTCTATGGCAGATTTAGAAGATTTAGCC
    ATTCCACATGCTGCTTTTAAAGATATTGTTAAAATGATGGATTATGGGGAAGGTCTAAAG
    AAGCATACCCGTGACGCTTTACGTCAATTGGAACAGAATTATCATGACTCATCGTCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0315 [new locus tag: NWMN_RS01795 ]
  • symbol: NWMN_0315
  • description: NADH-dependent flavin oxidoreductase Oye family protein
  • length: 399
  • theoretical pI: 6.56025
  • theoretical MW: 44691.3
  • GRAVY: -0.302506

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 148.2)
    and 3 more
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 100)
    3-isopropylmalate dehydratase, small subunit (TIGR02087; HMM-score: 11.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 11.6)
  • TheSEED  :
    • Probable NADH-dependent flavin oxidoreductase in cluster with COG2110
  • ⊞PFAM:
    TIM_barrel (CL0036) Oxidored_FMN; NADH:flavin oxidoreductase / NADH oxidase family (PF00724; HMM-score: 184)
    and 4 more
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 16.4)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 14.8)
    DHDPS; Dihydrodipicolinate synthetase family (PF00701; HMM-score: 12.8)
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9797
    • Cytoplasmic Membrane Score: 0.0065
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0134
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.057426
    • TAT(Tat/SPI): 0.002729
    • LIPO(Sec/SPII): 0.004003
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDLAYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAIKAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_0315&oldid=56486"