PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS08515 [old locus tag: SACOL1669 ]
pan locus tag^?: SAUPAN004210000
symbol: SACOL_RS08515
pan gene symbol^?: —
• synonym:
• product: O-methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS08515 [old locus tag: SACOL1669 ]
• symbol: SACOL_RS08515
• product: O-methyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1697218..1697856
• length: 639
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1697218..1697856) NCBI
• BioCyc: SACOL_RS08515 BioCyc
• MicrobesOnline: see SACOL1669

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGATGACCTAAATAAAAAATATTTAATAGATTTACATCAACATCAAAATAGTTCAATC
  GAAGTTTTGCGTGAATTTGCCGAGGTAAATGAAGTGCCAATTGTAGATCGTTTAACATTA
  GATTTAATTAAGCAATTAATTCGTATGAATAATGTTAAAAATATTTTAGAAATTGGTACA
  GCAATCGGCTATAGTTCTATGCAATTCGCTTCTATATCTGATGACATTCATGTCACAACG
  ATAGAGCGTAATGAAACGATGATTCAATATGCTAAACAAAATTTAGCTACTTATCATTTT
  GAAAACCAGGTTCGAATTATTGAAGGTAATGCTTTAGAGCAATTTGAAAATGTAAATGAC
  AAAGTTTATGATATGATATTTATTGATGCAGCAAAAGCGCAATCAAAGAAATTTTTTGAA
  ATATATACACCACTTTTAAAGCACCAAGGTCTCGTAATTACAGATAATGTTTTATATCAC
  GGTTTTGTATCGGATATTGGGATTGTTAGATCGAGAAATGTAAGACAAATGGTTAAAAAG
  GTTCAAGATTATAATGAGTGGTTAATAAAGCAACCAGGATATACAACGAATTTTTTAAAT
  ATAGACGATGGATTAGCGATTTCAATTAAAGGAGAATGA
  60
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⊟Protein

⊟General

• locus tag: SACOL_RS08515 [old locus tag: SACOL1669 ]
• symbol: SACOL_RS08515
• description: O-methyltransferase
• length: 212
• theoretical pI: 5.31765
• theoretical MW: 24526.8
• GRAVY: -0.255189

⊟Function

• ⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 40.9)
  Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 33.3)
  and 13 more
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 31.2)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 27.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 25.1)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 24.9)
  methyltransferase, ATP-grasp peptide maturase system (TIGR04188; HMM-score: 23.1)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 22.6)
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 21.7)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 18)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 17.7)
  methyltransferase, FxLD system (TIGR04364; HMM-score: 17.3)
  Unknown function Enzymes of unknown specificity tRNA (cmo5U34)-methyltransferase (TIGR00740; EC 2.1.1.-; HMM-score: 15.9)
  pseudaminic acid biosynthesis-associated methylase (TIGR03587; HMM-score: 15.5)
  methyltransferase, FkbM family (TIGR01444; HMM-score: 13.3)
• TheSEED: see SACOL1669
• ⊞PFAM:
  NADP_Rossmann (CL0063) Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 90.6)
  and 17 more
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 47.2)
  Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 46.5)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 35.9)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 30.1)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 29.6)
  Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 27.6)
  GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 26)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 25.9)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 21.8)
  CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 21.3)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 20.1)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 20)
  TRM5-TYW2_MTfase; TRM5/TYW2 methyltransferase domain (PF02475; HMM-score: 19)
  Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 18.8)
  Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 16.7)
  RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 16.4)
  Spermine_synth; Spermine/spermidine synthase domain (PF01564; HMM-score: 15.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9945
  • Cytoplasmic Membrane Score: 0.0048
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0004
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004664
  • TAT(Tat/SPI): 0.000192
  • LIPO(Sec/SPII): 0.000152
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446264411 NCBI
• RefSeq: WP_000342266 NCBI
• UniProt: see SACOL1669

⊟Protein sequence

• MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIKQLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFENQVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYHGFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE

⊟Experimental data

• experimentally validated: see SACOL1669
• protein localization: see SACOL1669
• quantitative data / protein copy number per cell: see SACOL1669
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1669

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications