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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS05935 [old locus tag: SA1044 ]
  • pan locus tag?: SAUPAN003481000
  • symbol: SA_RS05935
  • pan gene symbol?: pyrC
  • synonym:
  • product: dihydroorotase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS05935 [old locus tag: SA1044 ]
  • symbol: SA_RS05935
  • product: dihydroorotase
  • replicon: chromosome
  • strand: +
  • coordinates: 1180762..1182036
  • length: 1275
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1180762..1182036) NCBI
  • BioCyc: SA_RS05935 BioCyc
  • MicrobesOnline: see SA1044

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAATTAATTAAAAACGGTAAAGTATTACAAAATGGAGAATTACAACAAGCAGATATT
    TTAATTGATGGTAAGGTAATTAAACAAATTGCACCTGCAATTGAACCAAGCAATGGTGTT
    GACATCATAGATGCGAAAGGTCACTTTGTGTCACCTGGATTTGTCGATGTTCATGTTCAT
    TTACGTGAACCTGGTGGTGAATATAAAGAGACAATTGAAACTGGTACTAAAGCTGCTGCT
    AGAGGCGGATTTACAACTGTATGTCCAATGCCTAACACAAGACCGGTACCAGATTCTGTA
    GAACATTTTGAAGCTTTACAAAAATTAATCGATGACAATGCTCAAGTACGTGTATTACCT
    TATGCTTCAATTACAACACGTCAATTAGGTAAAGAATTGGTTGATTTCCCAGCACTAGTA
    AAAGAAGGTGCCTTTGCGTTTACAGATGACGGTGTAGGAGTACAAACTGCAAGCATGATG
    TATGAAGGCATGATTGAAGCTGCAAAAGTAAACAAAGCCATCGTAGCACACTGTGAAGAT
    AATTCATTAATCTATGGTGGTGCAATGCATGAAGGGAAACGCAGTAAAGAGTTAGGTATA
    CCAGGTATTCCAAACATTTGTGAATCTGTTCAAATCGCAAGAGATGTACTATTAGCTGAA
    GCAGCAGGTTGTCATTATCATGTATGTCATGTTTCTACTAAAGAAAGTGTTAGAGTCATT
    CGTGACGCTAAACGCGCAGGCATTCATGTTACAGCTGAAGTTACACCACACCATTTATTG
    TTAACAGAAGATGATATTCCTGGTAATAATGCCATTTATAAAATGAATCCACCATTGAGA
    AGTACTGAAGATAGAGAGGCTTTGTTAGAAGGGTTACTAGACGGTACAATTGACTGTATC
    GCAACAGACCATGCACCACATGCACGTGATGAAAAAGCACAACCAATGGAAAAAGCACCA
    TTCGGAATTGTTGGTAGTGAAACAGCATTCCCATTATTATATACGCATTTTGTAAAAAAT
    GGTGATTGGACATTACAACAATTAGTAGATTACTTAACAATTAAACCATGTGAGACATTT
    AATTTAGAATACGGCACATTAAAAGAAAATGGTTATGCAGATTTAACAATCATTGATTTA
    GATAGTGAACAAGAAATTAAAGGAGAAGATTTCTTATCAAAAGCAGATAATACACCATTT
    ATCGGCTATAAAGTTTATGGAAATCCGATCTTAACAATGGTTGAAGGCGAAGTTAAATTT
    GAGGGGGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1275

⊟Protein[edit | edit source]

Protein Data Bank: 3GRI

⊟General[edit | edit source]

  • locus tag: SA_RS05935 [old locus tag: SA1044 ]
  • symbol: SA_RS05935
  • description: dihydroorotase
  • length: 424
  • theoretical pI: 4.84608
  • theoretical MW: 46371.4
  • GRAVY: -0.22783

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 3.5.2.3?  ExPASy
    Dihydroorotase (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate
  • ⊞TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 471.8)
    and 12 more
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 245.6)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 201.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, homodimeric type (TIGR00856; EC 3.5.2.3; HMM-score: 47)
    Hypothetical proteins Conserved putative amidohydrolase, EF_0837/AHA_3915 family (TIGR03583; HMM-score: 43.1)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 33.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 28.1)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 27)
    Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 26.7)
    Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 25.9)
    Metabolism Central intermediary metabolism Amino sugars N-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 24.7)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 18.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 18.2)
  • TheSEED: see SA1044
  • ⊞PFAM:
    TIM_barrel (CL0036) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 139.4)
    and 2 more
    DHOase; Dihydro-orotase-like (PF12890; HMM-score: 110.9)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 37.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9972
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0024
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003924
    • TAT(Tat/SPI): 0.000187
    • LIPO(Sec/SPII): 0.000441
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIIDAKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEHFEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMMYEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLLAEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMNPPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLYTHFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFLSKADNTPFIGYKVYGNPILTMVEGEVKFEGDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: pyrR leader see SA1044

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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