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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01168
- pan locus tag?: SAUPAN003481000
- symbol: pyrC
- pan gene symbol?: pyrC
- synonym:
- product: dihydroorotase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_01168
- symbol: pyrC
- product: dihydroorotase
- replicon: chromosome
- strand: +
- coordinates: 1116581..1117855
- length: 1275
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3920919 NCBI
- RefSeq: YP_499707 NCBI
- BioCyc: G1I0R-1094 BioCyc
- MicrobesOnline: 1289621 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1261ATGAAATTAATTAAAAACGGTAAAGTATTACAAAATGGCGAATTACAACAAGCAGATATT
TTAATTGATGGTAAGGTAATTAAACAAATTGCACCTGCAATTGAACCAAGCAATGGTGTT
GACATCATAGATGCGAAAGGTCACTTTGTGTCACCTGGATTTGTCGATGTTCATGTTCAT
TTACGTGAACCTGGTGGTGAATATAAAGAGACAATTGAAACTGGTACTAAAGCTGCTGCT
AGAGGCGGATTTACAACTGTATGTCCAATGCCTAACACAAGACCGGTACCAGATTCTGTA
GAACATTTTGAAGCTTTACAAAAATTAATCGATGACAATGCTCAAGTACGTGTATTACCT
TATGCTTCAATTACAACACGTCAATTAGGTAAAGAATTGGTTGATTTCCCAGCACTAGTA
AAAGAAGGTGCCTTTGCGTTTACAGATGACGGTGTAGGAGTACAAACTGCAAGCATGATG
TATGAAGGCATGATTGAAGCTGCAAAAGTAAACAAAGCCATCGTAGCACACTGTGAAGAT
AATTCATTAATCTATGGTGGTGCAATGCATGAAGGGAAACGCAGTAAAGAGTTAGGTATA
CCAGGTATTCCAAACATTTGTGAATCTGTTCAAATCGCAAGAGATGTACTATTAGCTGAA
GCAGCAGGTTGTCATTATCATGTATGTCATGTTTCTACTAAAGAAAGTGTTAGAGTCATT
CGTGACGCTAAACGCGCAGGCATTCATGTTACAGCTGAAGTTACACCACACCATTTATTG
TTAACAGAAGATGATATTCCTGGTAATAATGCCATTTATAAAATGAATCCACCATTGAGA
AGTACTGAAGATAGAGAGGCTTTGTTAGAAGGGTTACTAGACGGTACAATTGACTGTATC
GCAACAGACCATGCACCACATGCACGTGATGAAAAAGCACAACCAATGGAAAAAGCACCA
TTCGGAATTGTTGGTAGTGAAACAGCATTCCCATTATTATATACGCATTTTGTAAAAAAT
GGTGATTGGACATTACAACAATTAGTAGATTACTTAACAATTAAACCATGTGAGACATTT
AATTTAGAATACGGCACATTAAAAGAAAATGGTTATGCAGATTTAACAATCATTGATTTA
GATAGTGAACAAGAAATTAAAGGAGAAGATTTCTTATCAAAAGCAGATAATACACCATTT
ATCGGCTATAAAGTTTATGGAAATCCGATCTTAACAATGGTTGAAGGCGAAGTTAAATTT
GAGGGGGATAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01168
- symbol: PyrC
- description: dihydroorotase
- length: 424
- theoretical pI: 4.84608
- theoretical MW: 46371.4
- GRAVY: -0.22783
⊟Function[edit | edit source]
- reaction: EC 3.5.2.3? ExPASyDihydroorotase (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate
- TIGRFAM: Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 471.8)and 12 moreallantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 245.6)dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 201.9)Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, homodimeric type (TIGR00856; EC 3.5.2.3; HMM-score: 47)Hypothetical proteins Conserved putative amidohydrolase, EF_0837/AHA_3915 family (TIGR03583; HMM-score: 43.1)Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 33.8)Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 28.1)putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 27)Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 26.7)Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 25.9)Central intermediary metabolism Amino sugars N-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 24.7)formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 18.4)Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 18.2)
- TheSEED :
- Dihydroorotase (EC 3.5.2.3)
- PFAM: Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 161.9)and 2 moreDHOase; Dihydro-orotase-like (PF12890; HMM-score: 120)Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 39.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Zn2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003924
- TAT(Tat/SPI): 0.000187
- LIPO(Sec/SPII): 0.000441
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIIDAKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEHFEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMMYEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLLAEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMNPPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLYTHFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFLSKADNTPFIGYKVYGNPILTMVEGEVKFEGDK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_01164 > SAOUHSC_01165 > pyrB > pyrC > SAOUHSC_01169 > carB > SAOUHSC_01171 > pyrEpredicted SigA promoter [3] : lspA > SAOUHSC_01163 > S480 > S481 > SAOUHSC_01164 > S482 > SAOUHSC_01165 > pyrB > pyrC > SAOUHSC_01169 > carB > S483 > SAOUHSC_01171 > pyrE > SAOUHSC_01173 > S484 > SAOUHSC_01174 > S485predicted SigA promoter [3] : S481 > SAOUHSC_01164 > S482 > SAOUHSC_01165 > pyrB > pyrC > SAOUHSC_01169 > carB > S483 > SAOUHSC_01171 > pyrE > SAOUHSC_01173 > S484 > SAOUHSC_01174 > S485
⊟Regulation[edit | edit source]
- regulator: pyrR leader (transcription antitermination) regulon
pyrR leader (5' cis-acting region) important in Pyrimidine metabolism; transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)