⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS03330 [old locus tag: SA0577 ]
pan locus tag^?: SAUPAN002492000
symbol: SA_RS03330
pan gene symbol^?: —
• synonym:
• product: recombinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS03330 [old locus tag: SA0577 ]
• symbol: SA_RS03330
• product: recombinase
• replicon: chromosome
• strand: +
• coordinates: 669396..669956
• length: 561
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (669396..669956) NCBI
• BioCyc: SA_RS03330 BioCyc
• MicrobesOnline: see SA0577

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATAAAGTAGAAGCGATTAAATTTAATGATGATATTGTTAAAATGTATGAAGCGCTC
  AAGATAAAATCTGAACGTGACTATTTATTCTTTAAGTTAGCTATACATAGTGGATTGAAA
  GTATCAGAATTATTAACAATTACAGTCTCTCAAGTTAAGAGACTAATTGAAAAGTGTACG
  TTATCAGAAATGTGTAAAGCACATTTTCATTCGTTGATTAAAATTAGGTTACCAGAAACA
  TTATCGAAAGAACTACTTCAATATATAGAGGACAGGAGTCTTTCGAATGAAGACGTTCTT
  TTTCAATCACTACGAACAAATCAAGTATTATCTAGACAGCAAGCATATCGAATAATTCAC
  CAAGCATCAATTGAAGCTGGTATAGATAATGTAGGACTAACGACATTGCGTAAGACATTT
  GCATATCATGCTTATCAAAAAGGTATACCTATACCAGTCATTCAAAAGTATTTAGGGCAT
  CAATCTGCTATTGAAACACTAAATTTTATCGGTTTAGAAAATGAGTGTGAACATAGTATT
  TATATTTCATTACAATTATAG
  60
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  540
  561
  

⊟Protein

⊟General

• locus tag: SA_RS03330 [old locus tag: SA0577 ]
• symbol: SA_RS03330
• description: recombinase
• length: 186
• theoretical pI: 8.45255
• theoretical MW: 21455.8
• GRAVY: -0.104839

⊟Function

• ⊞⊞TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 63.6)
  and 3 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 39.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 30.7)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 30.7)
• TheSEED: see SA0577
• ⊞⊞PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 79.9)
  and 2 more
  Phage_integr_3; Archaeal phage integrase (PF16795; HMM-score: 20.9)
  no clan defined ADSL_C; Adenylosuccinate lyase C-terminus (PF10397; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8207
  • Cytoplasmic Membrane Score: 0.1273
  • Cell wall & surface Score: 0.003
  • Extracellular Score: 0.049
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005027
  • TAT(Tat/SPI): 0.00212
  • LIPO(Sec/SPII): 0.00081
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446967659 NCBI
• RefSeq: WP_001044915 NCBI
• UniProt: see SA0577

⊟Protein sequence

• MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSELLTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQSLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGHQSAIETLNFIGLENECEHSIYISLQL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications