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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1954 [new locus tag: SACOL_RS10220 ]
  • pan locus tag?: SAUPAN004918000
  • symbol: SACOL1954
  • pan gene symbol?: —
  • synonym:
  • product: exonuclease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1954 [new locus tag: SACOL_RS10220 ]
  • symbol: SACOL1954
  • product: exonuclease
  • replicon: chromosome
  • strand: +
  • coordinates: 2014487..2015041
  • length: 555
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGATACAAGATGCGTTTGTTGCACTTGATTTTGAAACAGCAAATGGTAAACGTACAAGT
    ATTTGTTCTGTCGGAATGGTTAAAGTCATTGATAGTCAAATAACAGAAACATTTCATACT
    CTTGTGAATCCGCAAGACTATTTTTCACAACAAAATATTAAAATTCATGGCATACAACCA
    GAAGATGTTGAAAATGCACCTACGTTTGACTACGTATTTCCATATATGATGCAATTTATT
    GCAGATTTACCTGTTGTCGCACATAACGCGGCATTTGATATGAACGTCTTACATCAAAGC
    ATTCAAAATATTGGTTTACCAACTCCAAATTTAACTTACTTTTGTAGTTATCAACTTGCT
    AAAAGAACCGTTGATTCGTATCGATACGGTTTAAAACATATGATGGAGTTTTATCAATTA
    GATTTTCATGGTCATCATGATGCATTGAATGATGCCAAAGCATGCGCAATGATTACTTTT
    AGGCTACTGAAAAATTATGAAAATTTAACATATGTAACTAATATTTATGGTAAAAATCTA
    AAAGATAAAGGCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    555

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1954 [new locus tag: SACOL_RS10220 ]
  • symbol: SACOL1954
  • description: exonuclease
  • length: 184
  • theoretical pI: 6.2936
  • theoretical MW: 21120
  • GRAVY: -0.209239

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 87.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 81.4)
    Genetic information processing DNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 69.7)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 67.5)
    Genetic information processing Transcription RNA processing ribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 12.6)
  • TheSEED  :
    • DNA polymerase III polC-type (EC 2.7.7.7)
  • ⊞PFAM:
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 91.2)
    and 3 more
    Rv2179c-like; 3'-5' exoribonuclease Rv2179c-like domain (PF16473; HMM-score: 44)
    DNA_pol_A_exo1; 3'-5' exonuclease (PF01612; HMM-score: 20.8)
    no clan defined DUF1265; Protein of unknown function (DUF1265) (PF06887; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9832
    • Cytoplasmic Membrane Score: 0.0049
    • Cell wall & surface Score: 0.0026
    • Extracellular Score: 0.0093
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008011
    • TAT(Tat/SPI): 0.000547
    • LIPO(Sec/SPII): 0.0029
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNPQDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQNIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITFRLLKNYENLTYVTNIYGKNLKDKG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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