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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL

locus tag: SACOL1931 [new locus tag: SACOL_RS10100 ]

pan locus tag^?: SAUPAN004856000

symbol: recX

pan gene symbol^?: recX

synonym:
product: recombination regulator RecX

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL1931 [new locus tag: SACOL_RS10100 ]
symbol: recX
product: recombination regulator RecX
replicon: chromosome
strand: -
coordinates: 1995687..1996505
length: 819
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 3237532 NCBI
RefSeq: YP_186756 NCBI
BioCyc: see SACOL_RS10100
MicrobesOnline: 913410 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
GTGCCGAAGATTACTAAAATAGAAGTTCAAAAGAAAAACAAAGAACGGTTTAATCTTTTT
TTAGACGAACAATTTGAAATGGGTATAGATATTGATACATTAGTCAAATTTAATTTAAAA
AAAGGGCAACAACTTGAAGCTGCTGACATGGCAGAGATTCAAAAGTATGATCATTATCGC
ATAGGTTTAAATAAAGCAATCCAATATTTATCATATAAAAAGAGAACTGAAAAAGAAGTT
ATACAATATTTACAAAAAGAAGAGATATCAGAGCAAGCGATTTCTGAAGTGATTGAATAT
TGTTATCGCGAAAAGTTAATCGACCATCAAGATTATGCGGAAAGTTTAAAAAATACAATG
ATTCGCACGACAGATAAAGGACCTAAAATTTATCAACAAAAACTATATCAACTTGGTATA
GAACCAAATATCATTGAAATATTTACAGAACTTTATAGAGAACAACAGGAACTTGATGAT
ATTATCCAAATTGCTGAAAAAATATCTAAAACAAAAAAAGGGCCACAAAATAAAGTTAAA
GAAAAAGTAATGCAATCTTTAATTCAAAAAGGGTTTGAAATGGAAACCATTCATGCTGTA
TTGAATGAAATGGATTTTACACAAGATGAAGCGGTTTTAGACGATTTATTACAACGAGAT
TTAGAAAAAATTTATAATAAAAATCGAAAGAAGTACACGCAACAGAAATTAATTTCAAAA
ACTATCGAAGGCCTTATGAGAAAAGGATATAAATATGATAAAATTAAAGCTAAATTAGAA
GAAAGTGGTATTGCCGATGGAACAGAAGAAATTGAGTGA
60
120
180
240
300
360
420
480
540
600
660
720
780
819

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL1931 [new locus tag: SACOL_RS10100 ]
symbol: RecX
description: recombination regulator RecX
length: 272
theoretical pI: 5.9301
theoretical MW: 32240.8
GRAVY: -0.814338

⊟Function[edit | edit source]

TIGRFAM:
Metabolism Central intermediary metabolism Other 2,3-diketo-5-methylthio-1-phosphopentane phosphatase (TIGR01489; HMM-score: 8.8)
TheSEED :
- regulatory protein RecX
⊞PFAM:
HTH (CL0123) RecX_HTH1; RecX, first three-helix domain (PF21982; HMM-score: 58.1)
RecX_HTH3; RecX, third three-helix domain (PF21981; HMM-score: 47)
and 6 more
RecX_HTH2; RecX, second three-helix domain (PF02631; HMM-score: 32.5)
DUF6471; Domain of unknown function (DUF6471) (PF20075; HMM-score: 15.7)
no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 12.9)
HTH (CL0123) MCM5_C; MCM5, C-terminal domain (PF21933; HMM-score: 12.6)
Lin1244_N; Lin1244/Lin1753-like, N-terminal (PF14297; HMM-score: 9.5)
NusB (CL0633) NusB; NusB family (PF01029; HMM-score: 7.1)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9959
- Cytoplasmic Membrane Score: 0.0028
- Cell wall & surface Score: 0.001
- Extracellular Score: 0.0003
⊞
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002192
- TAT(Tat/SPI): 0.000124
- LIPO(Sec/SPII): 0.000204
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 57652092 NCBI
RefSeq: YP_186756 NCBI
UniProt: Q5HEQ1 UniProt

⊟Protein sequence[edit | edit source]

MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQLEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCYREKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDDIIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQRDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE

⊟Experimental data[edit | edit source]

experimentally validated: PeptideAtlas
protein localization: Cytoplasmic ^[1] ^[2]
quantitative data / protein copy number per cell: 88 ^[3]
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: SACOL1928 < SACOL1929 < SACOL1930 < recX

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib:

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊞Other Information[edit | edit source]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e)
↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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