From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0939 [new locus tag: NWMN_RS05260 ]
  • pan locus tag?: SAUPAN003284000
  • symbol: purM
  • pan gene symbol?: purM
  • synonym:
  • product: phosphoribosylaminoimidazole synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0939 [new locus tag: NWMN_RS05260 ]
  • symbol: purM
  • product: phosphoribosylaminoimidazole synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1042970..1043998
  • length: 1029
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCTAAAGCATATGAACAATCTGGTGTAAATATTCATGCTGGTTATGAAGCTGTAGAA
    AGAATGTCTAGTCATGTTAAACGTACGATGCGTAAAGAAGTTATCGGTGGTTTAGGTGGA
    TTCGGTGCTACATTTGATTTATCACAATTAAATATGACAGCGCCAGTTTTAGTTTCTGGA
    ACAGACGGAGTAGGTACGAAATTAAAACTAGCTATCGACTATGGTAAACATGATTCGATA
    GGTATCGATGCAGTCGCAATGTGTGTTAATGATATTTTAACGACAGGTGCAGAACCATTA
    TATTTTTTAGATTACATTGCTACAAACAAAGTTGTTCCTGAAGTTATTGAACAAATTGTT
    AAAGGTATTAGTGATGCATGTGTTGAAACGAATACTGCACTTATCGGTGGAGAGACTGCT
    GAAATGGGTGAAATGTATCACGAAGGCGAATATGATGTAGCTGGATTTGCTGTTGGAGCA
    GTTGAAAAGGATGACTATGTAGATGGTTCAGAAGTGAAAGAGGGACAAGTTGTTATAGGG
    CTTGCGTCAAGTGGCATTCATTCAAATGGATACAGTTTAGTGCGCAAATTAATTAATGAA
    TCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATCGATGTCTTTTTAGAA
    CCAACTAAATTATATGTCAAACCTGTACTTGCTTTGAAAAAAGAAGTTTCTATTAAGGCA
    ATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGA
    TATGCTGCTAGAATTGATACTACATCATTTCCAACACCAAAAATATTTGATTGGTTACAA
    CAACAAGGCAATATAGACACAAATGAAATGTATAACATTTTTAATATGGGTATTGGCTAT
    ACGGTTATCGTTGATGAAAAAGATGTATCACGCGCTTTGAAGATTTTAGCAGAACAAAAT
    GTGGAAGCCTATCAAATTGGTCATATTGTGAAAAATGAGTCAACTGCAATTGAATTGTTG
    GGGGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1029

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0939 [new locus tag: NWMN_RS05260 ]
  • symbol: PurM
  • description: phosphoribosylaminoimidazole synthetase
  • length: 342
  • theoretical pI: 4.52335
  • theoretical MW: 37016.8
  • GRAVY: -0.0175439

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.3.3.1?  ExPASy
    Phosphoribosylformylglycinamidine cyclo-ligase ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
  • ⊞TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 429.2)
    and 8 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 41.6)
    hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 41.3)
    Metabolism Energy metabolism Methanogenesis putative methanogenesis marker protein 2 (TIGR03267; HMM-score: 32.6)
    selenide, water dikinase (TIGR00476; EC 2.7.9.3; HMM-score: 24.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 22.2)
    Unknown function Enzymes of unknown specificity AIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 17.6)
    Unknown function Enzymes of unknown specificity AIR synthase-related protein, MSMEG_0567 C-terminal family (TIGR04050; HMM-score: 17.3)
    bacteriocin-type signal sequence (TIGR01847; HMM-score: 11)
  • TheSEED  :
    • Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1)
  • ⊞PFAM:
    no clan defined AIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 136.7)
    and 2 more
    PurM_N-like (CL0804) AIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 89.4)
    GlnB-like (CL0089) DUF2007; Putative prokaryotic signal transducing protein (PF09413; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9883
    • Cytoplasmic Membrane Score: 0.0025
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.009
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025668
    • TAT(Tat/SPI): 0.024848
    • LIPO(Sec/SPII): 0.002504
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGATFDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYFLDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGAVEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVFLEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIFDWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNESTAIELLGV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_0939&oldid=60276"