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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0723 [new locus tag: SACOL_RS03715 ]
  • pan locus tag?: SAUPAN002545000
  • symbol: SACOL0723
  • pan gene symbol?: cxcA
  • synonym:
  • product: LysM domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0723 [new locus tag: SACOL_RS03715 ]
  • symbol: SACOL0723
  • product: LysM domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 747932..748729
  • length: 798
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    TTGAAAAAATTAGCATTTGCAATAACAGCAACATCTGGTGCAGCTGCATTTTTAACGCAT
    CATGATGCACAAGCTTCTACACAACATACAGTACAATCTGGTGAATCATTATGGAGTATT
    GCTCAAAAATACAACACTTCAGTAGAGAGTATTAAACAAAATAACCAATTAGATAACAAC
    TTGGTATTCCCTGGTCAAGTTATCTCAGTAGGTGGAAGTGATGCACAAAATACGTCAAAC
    ACTTCTCCACAAGCTGGTTCAGCATCATCTCATACTGTACAAGCTGGTGAATCATTAAAT
    ATCATTGCTAGCAGATATGGTGTTTCAGTTGATCAATTAATGGCAGCCAATAACTTACGT
    GGTTATTTAATTATGCCTAACCAAACATTACAAATTCCTAATGGTGGATCAGGTGGTACA
    ACACCAACAGCTACAACAGGTAGCAATGGCAATGCATCATCTTTTAATCACCAAAATTTA
    TACACTGCTGGTCAATGTACATGGTACGTATTTGACCGTCGTGCTCAAGCTGGTAGTCCA
    ATTAGCACATATTGGTCAGACGCTAAGTATTGGGCTGGTAACGCAGCTAATGATGGTTAC
    CAAGTAAACAACACACCATCAGTTGGTTCAATTATGCAAAGCACACCTGGTCCATATGGT
    CATGTTGCTTATGTTGAACGTGTCAATGGTGATGGTAGTATCTTGATTTCTGAAATGAAT
    TACACATATGGTCCATACAATATGAACTACCGTACAATTCCAGCTTCAGAAGTTTCTAGC
    TATGCATTCATCCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    798

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0723 [new locus tag: SACOL_RS03715 ]
  • symbol: SACOL0723
  • description: LysM domain-containing protein
  • length: 265
  • theoretical pI: 6.62611
  • theoretical MW: 28186.6
  • GRAVY: -0.392075

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 63.9)
    and 1 more
    Cellular processes Cellular processes Sporulation and germination stage VI sporulation protein D (TIGR02907; HMM-score: 41.2)
  • TheSEED  :
    • N-acetylmuramoyl-L-alanine amidase SAV0665 (EC 3.5.1.28)
  • ⊞PFAM:
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 106.5)
    and 6 more
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 78.6)
    LysM (CL0187) LysM3_LYK4_5; LYK4/5 LysM3 domain (PF23473; HMM-score: 40.7)
    LysM_RLK; LysM domain (PF23577; HMM-score: 22.3)
    LysM1_NFP_LYK; NFP/LYK LysM1 domain (PF23446; HMM-score: 18.9)
    LysM2_CERK1_LYK3_4_5; LYK3/4/5 LysM2 domain (PF23472; HMM-score: 17.7)
    NMB0315_dom_I; NMB0315-like, domain I (PF22310; HMM-score: 17.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.21
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0.057
    • Extracellular Score: 0.9429
  • ⊞LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: HDAQASTQ
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 25 aa
    • SP(Sec/SPI): 0.982953
    • TAT(Tat/SPI): 0.004467
    • LIPO(Sec/SPII): 0.002627
    • Cleavage Site: CS pos: 25-26. AQA-ST. Pr: 0.9495
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKYNTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNIIASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNLYTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGPYGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Viktor H Mebus, Larissa M Busch, Morten Børre, Tobias K Nielsen, Martin Saxtorph Bojer, Camilla Henriksen, Maria D Barbuti, Danae M Angeles, Kamilla Brejndal, Stephan Michalik, Manuela Gesell Salazar, Morten Kjos, Uwe Völker, Birgitte H Kallipolitis, Dorte Frees
Temperature-dependent regulation of bacterial cell division hydrolases by the coordinated action of a regulatory RNA and the ClpXP protease.
Cell Surf: 2025, 13;100144
[PubMed:40496198] [WorldCat.org] [DOI] (I e)

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