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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0651 [new locus tag: SAUSA300_RS03490 ]
  • pan locus tag?: SAUPAN002545000
  • symbol: SAUSA300_0651
  • pan gene symbol?: cxcA
  • synonym:
  • product: CHAP domain-contain protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0651 [new locus tag: SAUSA300_RS03490 ]
  • symbol: SAUSA300_0651
  • product: CHAP domain-contain protein
  • replicon: chromosome
  • strand: -
  • coordinates: 726462..727259
  • length: 798
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    TTGAAAAAATTAGCATTTGCAATAACAGCAACATCTGGTGCAGCTGCATTTTTAACGCAT
    CATGATGCACAAGCTTCTACACAACATACAGTACAATCTGGTGAATCATTATGGAGTATT
    GCTCAAAAATACAACACTTCAGTAGAGAGTATTAAACAAAATAACCAATTAGATAACAAC
    TTGGTATTCCCTGGTCAAGTTATCTCAGTAGGTGGAAGTGATGCACAAAATACGTCAAAC
    ACTTCTCCACAAGCTGGTTCAGCATCATCTCATACTGTACAAGCTGGTGAATCATTAAAT
    ATCATTGCTAGCAGATATGGTGTTTCAGTTGATCAATTAATGGCAGCCAATAACTTACGT
    GGTTATTTAATTATGCCTAACCAAACATTACAAATTCCTAATGGTGGATCAGGTGGTACA
    ACACCAACAGCTACAACAGGTAGCAATGGCAATGCATCATCTTTTAATCACCAAAATTTA
    TACACTGCTGGTCAATGTACATGGTACGTATTTGACCGTCGTGCTCAAGCTGGTAGTCCA
    ATTAGCACATATTGGTCAGACGCTAAGTATTGGGCTGGTAACGCAGCTAATGATGGTTAC
    CAAGTAAACAACACACCATCAGTTGGTTCAATTATGCAAAGCACACCTGGTCCATATGGT
    CATGTTGCTTATGTTGAACGTGTCAATGGTGATGGTAGTATCTTGATTTCTGAAATGAAT
    TACACATATGGTCCATACAATATGAACTACCGTACAATTCCAGCTTCAGAAGTTTCTAGC
    TATGCATTCATCCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    798

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0651 [new locus tag: SAUSA300_RS03490 ]
  • symbol: SAUSA300_0651
  • description: CHAP domain-contain protein
  • length: 265
  • theoretical pI: 6.62611
  • theoretical MW: 28186.6
  • GRAVY: -0.392075

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination spore coat assembly protein SafA (TIGR02899; HMM-score: 63.9)
    and 1 more
    Cellular processes Cellular processes Sporulation and germination stage VI sporulation protein D (TIGR02907; HMM-score: 41.2)
  • TheSEED  :
    • N-acetylmuramoyl-L-alanine amidase SAV0665 (EC 3.5.1.28)
  • PFAM:
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 106.5)
    and 6 more
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 78.6)
    LysM (CL0187) LysM3_LYK4_5; LYK4/5 LysM3 domain (PF23473; HMM-score: 40.7)
    LysM_RLK; LysM domain (PF23577; HMM-score: 22.3)
    LysM1_NFP_LYK; NFP/LYK LysM1 domain (PF23446; HMM-score: 18.9)
    LysM2_CERK1_LYK3_4_5; LYK3/4/5 LysM2 domain (PF23472; HMM-score: 17.7)
    NMB0315_dom_I; NMB0315-like, domain I (PF22310; HMM-score: 17.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.21
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0.057
    • Extracellular Score: 0.9429
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: HDAQASTQ
  • SignalP: Signal peptide SP(Sec/SPI) length 25 aa
    • SP(Sec/SPI): 0.982953
    • TAT(Tat/SPI): 0.004467
    • LIPO(Sec/SPII): 0.002627
    • Cleavage Site: CS pos: 25-26. AQA-ST. Pr: 0.9495
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKYNTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNIIASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNLYTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGPYGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Viktor H. Mebus, Larissa M. Busch, Morten Børre, Tobias K. Nielsen, Martin Saxtorph Bojer, Camilla Henriksen, Maria D. Barbuti, Danae M. Angeles, Kamilla Brejndal, Stephan Michalik, Manuela Gesell Salazar, Morten Kjos, Uwe Völker, Birgitte H. Kallipolitis, Dorte Frees
Temperature-dependent regulation of bacterial cell division hydrolases by the coordinated action of a regulatory RNA and the ClpXP protease
[DOI]

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