From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08665 [old locus tag: NWMN_1545 ]
  • pan locus tag?: SAUPAN004249000
  • symbol: NWMN_RS08665
  • pan gene symbol?: thrR
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS08665 [old locus tag: NWMN_1545 ]
  • symbol: NWMN_RS08665
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1713255..1713710
  • length: 456
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1713255..1713710) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1545

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGACAATAAAGATTATAAAAAGTTTTATTTAATTAGAGAAGATGTCTTGCCTGAATCC
    GTGGTTAAAACATTGAAGATTAAAGATGCCTTAAAAAGTGATCCGACATTGTCCATTTAT
    GATGCCGTTAAACAGTTTGATCTATCTAGAAGTGCTTTTTATAAATATAGAGAAACGATA
    TTTCCAGTAGACGATAAAATGCTTGACCATCGAGAATTTACATTAATTTTATATGTAACT
    GATATTGTTGGTATGTTGGCACGTGTACTAGATGTTATATCAAAGTTAGAACTATCTGTA
    TTAACGATTCATCAAAGTATTCCAATGGAAGAAAAAGCAACAATAACATTATCACTGAAT
    GCTAAATCTAAAGAAACTTCAGTAGAAGATGTTATTGGCGCTTTGAGAAATTTAGATTAT
    GTATCAAAAGTAGAATTAATTAGTATGAGTATGTAA
    60
    120
    180
    240
    300
    360
    420
    456

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS08665 [old locus tag: NWMN_1545 ]
  • symbol: NWMN_RS08665
  • description: hypothetical protein
  • length: 151
  • theoretical pI: 5.3108
  • theoretical MW: 17343.1
  • GRAVY: -0.0278146

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 13.1)
  • TheSEED: see NWMN_1545
  • ⊞PFAM:
    ACT (CL0070) ACT_4; ACT domain (PF13291; HMM-score: 24.9)
    ACT; ACT domain (PF01842; HMM-score: 21.5)
    and 5 more
    bHLH-TF_ACT-like_plant; Plant bHLH transcription factor, ACT-like domain (PF22754; HMM-score: 19.1)
    ACT_5; ACT domain (PF13710; HMM-score: 14.9)
    no clan defined DUF5896; Family of unknown function (DUF5896) (PF19248; HMM-score: 14.2)
    ACT (CL0070) ACT_AHAS_ss; AHAS small subunit-like ACT domain (PF22629; HMM-score: 13.3)
    HTH (CL0123) HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7532
    • Cytoplasmic Membrane Score: 0.0443
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.2019
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002097
    • TAT(Tat/SPI): 0.000135
    • LIPO(Sec/SPII): 0.000449
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVKQFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLTIHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_RS08665&oldid=83757"