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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01278
  • pan locus tag?: SAUPAN003613000
  • symbol: SAOUHSC_01278
  • pan gene symbol?: glpD
  • synonym:
  • product: aerobic glycerol-3-phosphate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01278
  • symbol: SAOUHSC_01278
  • product: aerobic glycerol-3-phosphate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1234935..1236608
  • length: 1674
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCATTGTCTACTTTTAAGAGAGAACATATTAAAAAGAATTTAAGAAATGATGAATAT
    GATTTAGTAATTATTGGTGGCGGTATTACAGGTGCAGGTATTGCACTAGACGCGAGTGAA
    AGAGGAATGAAAGTTGCATTAGTTGAAATGCAAGACTTTGCACAAGGAACAAGCTCAAGA
    TCTACAAAATTAGTCCATGGTGGTTTGCGTTACTTAAAACAATTCCAAATTGGAGTAGTT
    GCCGAAACTGGTAAAGAACGTGCGATTGTTTATGAAAATGGGCCTCATGTTACGACTCCA
    GAGTGGATGCTTTTACCAATGCATAAAGGTGGAACATTTGGTAAATTCTCAACATCAATT
    GGTTTAGGAATGTATGATCGTTTAGCAGGTGTTAAGAAGTCTGAACGTAAAAAAATGTTA
    TCTAAAAAAGAAACTTTAGCTAAAGAACCATTAGTTAAAAAAGAAGGTCTAAAAGGCGGC
    GGTTACTATGTTGAATATCGTACTGACGATGCGCGTTTAACTATTGAAGTTATGAAGCGT
    GCTGCTGAAAAAGGCGCAGAAATTATCAACTATACTAAATCTGAACACTTCACTTATGAT
    AAAAATCAACAAGTAAATGGTGTTAAAGTTATAGATAAATTAACTAATGAAAATTATACA
    ATTAAGGCTAAAAAAGTGGTTAATGCAGCAGGTCCATGGGTTGATGATGTTAGAAGTGGT
    GATTATGCACGCAATAATAAAAAATTACGTTTAACTAAAGGTGTACATGTTGTTATTGAT
    CAATCAAAATTCCCATTAGGTCAAGCAGTATACTTTGATACTGAAAAAGATGGAAGAATG
    ATTTTTGCAATTCCACGTGAAGGAAAAGCGTATGTAGGTACTACAGATACATTCTATGAC
    AATATCAAATCTTCACCATTAACTACACAAGAAGACAGAGACTATTTAATCGATGCGATT
    AATTACATGTTCCCTAGTGTTAATGTTACAGATGAAGATATTGAATCAACATGGGCAGGA
    ATTAGACCATTAATTTACGAAGAAGGCAAAGACCCTTCTGAAATCTCTCGTAAGGATGAA
    ATTTGGGAAGGTAAATCAGGTTTATTAACTATTGCAGGTGGTAAATTAACAGGCTATCGT
    CACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTA
    ACATTTAGTCCATGTAATACAAAAGGTCTGGCAATTTCAGGTGGCGATGTAGGTGGTAGC
    AAGAACTTTGATGCGTTTGTAGAGCAAAAAGTAGATGTAGCTAAAGGATTCGGCATTGAT
    GAAGATGTTGCAAGACGTTTAGCATCTAAATATGGTTCAAATGTTGATGAATTGTTCAAC
    ATTGCGCAAACATCTCAATACCATGATAGCAAGTTACCATTAGAAATTTATGTAGAACTT
    GTTTATAGTATTCAACAAGAAATGGTATACAAACCTAACGATTTCTTAGTTCGTCGTTCT
    GGTAAAATGTATTTCAATATTAAAGATGTATTAGATTATAAAGATGCTGTCATCGATATT
    ATGGCAGATATGCTTGATTACTCTCCAGCTCAAATTGAAGCATATACTGAAGAAGTTGAG
    CAAGCAATTAAAGAAGCGCAACATGGAAATAATCAACCAGCAGTTAAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1674

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01278
  • symbol: SAOUHSC_01278
  • description: aerobic glycerol-3-phosphate dehydrogenase
  • length: 557
  • theoretical pI: 7.03905
  • theoretical MW: 62387.5
  • GRAVY: -0.490844

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 1.1.5.3?  ExPASy
    Glycerol-3-phosphate dehydrogenase sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol
  • ⊞TIGRFAM:
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, A subunit (TIGR03377; EC 1.1.5.3; HMM-score: 175.5)
    and 26 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 48.2)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 33.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 31.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 27.4)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 23.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 22)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 19.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 18.9)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 18.3)
    lycopene cyclase family protein (TIGR01790; HMM-score: 18)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 16.1)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 16.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 14.7)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 14.6)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, monomeric form (TIGR01377; HMM-score: 14.2)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 13.5)
    putative aminophosphonate oxidoreductase (TIGR03329; HMM-score: 13.2)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, beta subunit family (TIGR01373; HMM-score: 12.3)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.2)
    Metabolism Energy metabolism Anaerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.2)
    Metabolism Energy metabolism Aerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 12.2)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase, flavoprotein subunit (TIGR01816; HMM-score: 11.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 11.6)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 11.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other C-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 10.2)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 9.7)
  • ⊞TheSEED  :
    • Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)
    Carbohydrates Sugar alcohols Glycerol and Glycerol-3-phosphate Uptake and Utilization  Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)
    and 2 more
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)
    Respiration Electron donating reactions Respiratory dehydrogenases 1  Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)
  • ⊞PFAM:
    NADP_Rossmann (CL0063) DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 205.5)
    and 13 more
    Fer2_BFD (CL0667) DAO_C; C-terminal domain of alpha-glycerophosphate oxidase (PF16901; HMM-score: 86.9)
    NADP_Rossmann (CL0063) FAD_binding_2; FAD binding domain (PF00890; HMM-score: 25.8)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 24.7)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 24.7)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 22.4)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 20.7)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 20.1)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 17.5)
    GMC_oxred_N; GMC oxidoreductase (PF00732; HMM-score: 15.9)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 15.5)
    Thi4; Thi4 family (PF01946; HMM-score: 15.1)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 13.3)
    HI0933_like; HI0933-like protein Rossmann domain (PF03486; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.6962
    • Cytoplasmic Membrane Score: 0.2967
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0069
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007739
    • TAT(Tat/SPI): 0.004601
    • LIPO(Sec/SPII): 0.0012
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMKVALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEWMLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGGGYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNENYTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEKDGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDIESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSKRLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASKYGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIKDVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: CcpA* regulon

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser:  [3] 
    Expression Data Browser
    ⊟Multi-gene expression profiles



    Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Jump up to: 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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