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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS07625 [old locus tag: SACOL1497 ]
- pan locus tag?: SAUPAN003917000
- symbol: SACOL_RS07625
- pan gene symbol?: papS
- synonym:
- product: CCA-adding enzyme
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS07625 [old locus tag: SACOL1497 ]
- symbol: SACOL_RS07625
- product: CCA-adding enzyme
- replicon: chromosome
- strand: -
- coordinates: 1538264..1539466
- length: 1203
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1201ATGGATAAATCATTATTTGAACAGGCAAGGCCTATATTAGAACAAATTCAAGACAATGGT
TTTGAAGCATATTATGTAGGTGGCTCTGTAAGAGATTATGTCATGGGAAGAAATATTCAT
GATATAGATATCACAACAAGTGCAACGCCGGATGAAATAGAATCTATCTTTAGTCATACG
ATACCTGTAGGTAAAGAACATGGCACGATAAATGTAGTTTTTAATGATGAAAATTATGAA
GTGACAACATTCCGGGCTGAAGAAGATTATGTCGATCACCGTAGACCAAGTGGTGTTACA
TTTGTTCGTGATTTATACGAAGATTTGCAACGACGAGATTTCACGATGAATGCGATAGCA
ATGGATACAGCATACAAATTGTATGATTATTTTGATGGTCAACAAGATATTAATAATCGA
ATAATAAGAACTGTAGGTATAGCTGAGGAACGATTCCAAGAAGATGCTTTACGTATGATT
CGATGTTTAAGGTTCCAGTCACAATTATCATTTGATATTGCAACGGAAACATTCGAAGCG
ATGCGTATACAAATGGCAGATATTAAATTTTTATCAATTGAGCGTATAGTGATTGAACTA
ACTAAATTAATGCGAGGTATTAATGTTGAAAAGAGTTTTAATCATTTAAAATCGCTGAAA
GCATTTAATTATATGCCGTATTTCGAACATCTTGATATGAATCAAATTAATGTAACTGAA
GCAATTGATTTAGAATTGTTGATTGCTATAGTATCAGTTAAATTTGATATTAATTACTCA
TTGAAGCCTTTAAAGCTAAGTAACCGACAAGTAAAAGATATCAATCAATATATTCAAATT
ATGAATGCATTACCAAGTATTATTACAAAAGAACAATTAAAAATGTTTGTTTATGATTAT
GATACGCATCTCATTAAAAATGTAATGGTTGCAGCAGACGTGTTAAAGGCAAATGATATT
CAAGGACATGAACCATTAATCGTTAACCTTCAAACGATTGATGAAACATTACATCGTTTA
CCTATGCATAATAGAAAAGACATGATGGTTAATGGCGGTGTACTTATGGCACATTTAAAT
GCCAAAAGTGGTCCGTGGTTAAAAGATGTGCTAAGACAAATTGAGATAGCGATTGTAACA
GGTAAAGTAAGCAACGAAGAAACTGAAATTTTGAAATGGGTGGATAATCATGTCAAAATA
TAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS07625 [old locus tag: SACOL1497 ]
- symbol: SACOL_RS07625
- description: CCA-adding enzyme
- length: 400
- theoretical pI: 5.1044
- theoretical MW: 46435.9
- GRAVY: -0.247
⊟Function[edit | edit source]
- ⊞reaction: EC 2.7.7.72? ExPASy
- TIGRFAM: poly(A) polymerase (TIGR01942; EC 2.7.7.19; HMM-score: 161.7)Protein synthesis tRNA and rRNA base modification CCA tRNA nucleotidyltransferase (TIGR02692; EC 2.7.7.72; HMM-score: 161.1)
- TheSEED: see SACOL1497
- ⊞PFAM: NTP_transf (CL0260) PolyA_pol; Poly A polymerase head domain (PF01743; HMM-score: 132.5)and 2 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Mg2+
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMIRCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
⊟Experimental data[edit | edit source]
- experimentally validated: see SACOL1497
- protein localization: see SACOL1497
- quantitative data / protein copy number per cell:
- ⊟interaction partners:
SACOL_RS11695 30S ribosomal protein S5 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)