PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1539 [new locus tag: SA_RS08665 ]
pan locus tag^?: SAUPAN004370000
symbol: SA1539
pan gene symbol^?: ezrA
• synonym:
• product: septation ring formation regulator EzrA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1539 [new locus tag: SA_RS08665 ]
• symbol: SA1539
• product: septation ring formation regulator EzrA
• replicon: chromosome
• strand: -
• coordinates: 1758381..1760075
• length: 1695
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124384 NCBI
• RefSeq: NP_374827 NCBI
• BioCyc: see SA_RS08665
• MicrobesOnline: 103853 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGTGTTATATATCATTTTGGCAATAATTGTGATTATATTGATTGCTGTAGGTGTATTA
  TTCTATTTACGTTCAAATAAACGACAAATTATTGAAAAAGCAATCGAACGTAAAAATGAA
  ATTGAGACGTTACCTTTTGATCAAAACCTTGCACAATTATCTAAGTTGAATTTAAAAGGT
  GAAACAAAAACGAAATACGATGCAATGAAAAAGGACAACGTAGAAAGTACAAATAAGTAT
  CTAGCTCCTGTGGAAGAAAAAATCCATAATGCTGAGGCTTTATTAGATAAATTTAGTTTC
  AACGCATCTCAAAGTGAAATTGATGATGCAAATGAGTTGATGGATAGTTACGAACAAAGC
  TATCAGCAACAATTAGAAGATGTAAATGAAATTATTGCGTTATACAAAGATAATGATGAA
  TTATATGACAAATGTAAGGTTGATTATCGTGAAATGAAACGTGATGTTTTAGCAAATCGT
  CATCAATTTGGTGAGGCAGCAAGTCTACTTGAAACTGAAATTGAAAAGTTTGAGCCAAGG
  TTAGAGCAATATGAAGTACTAAAAGCTGATGGTAATTATGTACAAGCGCACAACCATATA
  GCTGCCTTGAATGAACAAATGAAACAGCTAAGATCTTATATGGAAGAAATACCAGAATTA
  ATTAGAGAAACTCAAAAAGAATTACCTGGTCAATTCCAAGATTTAAAATATGGTTGCCGT
  GATCTTAAAGTTGAAGGGTATGATCTGGATCACGTAAAAGTAGACAGTACATTACAAAGC
  TTAAAAACAGAGCTTAGTTTCGTTGAACCATTAATTAGCCGCTTAGAATTAGAAGAAGCT
  AATGATAAACTAGCTAATATCAATGATAAGTTAGATGACATGTATGATTTAATTGAACAT
  GAAGTTAAAGCTAAAAATGATGTCGAAGAAACAAAAGATATCATTACGGATAACTTATTC
  AAAGCTAAAGACATGAATTATACATTGCAAACAGAAATTGAATATGTACGTGAAAACTAC
  TATATAAATGAATCTGATGCTCAAAGTGTTCGTCAATTTGAAAATGAAATTCAAAGTTTA
  ATTTCTGTATATGATGATATTTTAAAAGAAATGTCTAAATCTGCTGTACGATATAGCGAG
  GTTCAGGATAATTTACAATATTTAGAAGATCATGTCACAGTTATTAATGACAAACAAGAA
  AAGCTACAAAATCATCTGATTCAATTGCGTGAAGATGAAGCAGAAGCAGAAGACAATCTG
  CTACGAGTACAATCGAAGAAAGAAGAAGTGTATCGTCGATTACTTGCTTCTAACTTAACA
  AGCGTTCCTGAAAGGTTTATCATCATGAAAAATGAAATTGATCATGAAGTTCGTGATGTT
  AACGAACAATTTAGTGAACGTCCAATACACGTTAAACAGTTAAAAGATAAAGTGTCTAAA
  ATTGTGATTCAAATGAATACATTTGAAGATGAAGCAAATGATGTTCTTGTTAATGCTGTT
  TATGCAGAGAAATTAATTCAATATGGAAATAGATATCGTAAGGACTATAGCAATGTTGAT
  AAGAGCTTAAATGAAGCTGAACGATTATTTAAAAATAATCGCTATAAGCGTGCGATTGAA
  ATTGCAGAGCAAGTTCTTGAAAGTGTTGAGCCAGGTGTCACTAAACATATTGAAGAAGAA
  GTTATTAAGCAATAG
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⊟Protein

⊟General

• locus tag: SA1539 [new locus tag: SA_RS08665 ]
• symbol: SA1539
• description: septation ring formation regulator EzrA
• length: 564
• theoretical pI: 4.50272
• theoretical MW: 66227.1
• GRAVY: -0.682092

⊟Function

• ⊞TIGRFAM:
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 23.2)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 23.2)
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 22.7)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 22.7)
  and 2 more
  Genetic information processing Protein fate Protein folding and stabilization cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 5.9)
  Metabolism Energy metabolism Electron transport cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 5.9)
• TheSEED  :
  • Septation ring formation regulator EzrA
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Control of cell elongation - division cycle in Bacilli  Septation ring formation regulator EzrA
• ⊞PFAM:
  no clan defined EzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 537.2)
  and 12 more
  GpW_gp25-like (CL0763) GPW_gp25; Baseplate wedge protein gp25 (PF04965; HMM-score: 19.5)
  no clan defined PI3K_P85_iSH2; Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain (PF16454; HMM-score: 19)
  Csm6_6H; Csm6 6H domain (PF22205; HMM-score: 15.7)
  Ac110_PIF; Per os infectivity factor AC110 (PF07280; HMM-score: 15.6)
  DUF6249; Domain of unknown function (DUF6249) (PF19762; HMM-score: 13.4)
  DUF2106; Predicted membrane protein (DUF2106) (PF09879; HMM-score: 12.5)
  ASFV_J13L; African swine fever virus J13L protein (PF05568; HMM-score: 12.4)
  FRB_dom; FKBP12-rapamycin binding domain (PF08771; HMM-score: 11.2)
  IT (CL0182) ArsP_1; Predicted permease (PF03773; HMM-score: 10.3)
  no clan defined Med9; RNA polymerase II transcription mediator complex subunit 9 (PF07544; HMM-score: 10)
  DUF7237; Family of unknown function (DUF7237) (PF23884; HMM-score: 8.6)
  KNTase_C (CL0291) DUF5667; Domain of unknown function (DUF5667) (PF18915; HMM-score: 7.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.94
  • Cytoplasmic Membrane Score: 2.06
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helix: 1
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.9889
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0109
• ⊞LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.026729
  • TAT(Tat/SPI): 0.000705
  • LIPO(Sec/SPII): 0.007408
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15927294 NCBI
• RefSeq: NP_374827 NCBI
• UniProt: P64003 UniProt

⊟Protein sequence

• MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETLPFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNASQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANRHQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIPELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLELEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIEYVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHVTVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMKNEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQYGNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQVLESVEPGVTKHIEEEVIKQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications