PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS08665 [old locus tag: SA1539 ]
• pan locus tag^?: SAUPAN004370000
• symbol: SA_RS08665
• pan gene symbol^?: ezrA
• synonym:
• product: septation ring formation regulator EzrA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08665 [old locus tag: SA1539 ]
• symbol: SA_RS08665
• product: septation ring formation regulator EzrA
• replicon: chromosome
• strand: -
• coordinates: 1758381..1760075
• length: 1695
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1758381..1760075) NCBI
• BioCyc: SA_RS08665 BioCyc
• MicrobesOnline: see SA1539

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGTGTTATATATCATTTTGGCAATAATTGTGATTATATTGATTGCTGTAGGTGTATTA
  TTCTATTTACGTTCAAATAAACGACAAATTATTGAAAAAGCAATCGAACGTAAAAATGAA
  ATTGAGACGTTACCTTTTGATCAAAACCTTGCACAATTATCTAAGTTGAATTTAAAAGGT
  GAAACAAAAACGAAATACGATGCAATGAAAAAGGACAACGTAGAAAGTACAAATAAGTAT
  CTAGCTCCTGTGGAAGAAAAAATCCATAATGCTGAGGCTTTATTAGATAAATTTAGTTTC
  AACGCATCTCAAAGTGAAATTGATGATGCAAATGAGTTGATGGATAGTTACGAACAAAGC
  TATCAGCAACAATTAGAAGATGTAAATGAAATTATTGCGTTATACAAAGATAATGATGAA
  TTATATGACAAATGTAAGGTTGATTATCGTGAAATGAAACGTGATGTTTTAGCAAATCGT
  CATCAATTTGGTGAGGCAGCAAGTCTACTTGAAACTGAAATTGAAAAGTTTGAGCCAAGG
  TTAGAGCAATATGAAGTACTAAAAGCTGATGGTAATTATGTACAAGCGCACAACCATATA
  GCTGCCTTGAATGAACAAATGAAACAGCTAAGATCTTATATGGAAGAAATACCAGAATTA
  ATTAGAGAAACTCAAAAAGAATTACCTGGTCAATTCCAAGATTTAAAATATGGTTGCCGT
  GATCTTAAAGTTGAAGGGTATGATCTGGATCACGTAAAAGTAGACAGTACATTACAAAGC
  TTAAAAACAGAGCTTAGTTTCGTTGAACCATTAATTAGCCGCTTAGAATTAGAAGAAGCT
  AATGATAAACTAGCTAATATCAATGATAAGTTAGATGACATGTATGATTTAATTGAACAT
  GAAGTTAAAGCTAAAAATGATGTCGAAGAAACAAAAGATATCATTACGGATAACTTATTC
  AAAGCTAAAGACATGAATTATACATTGCAAACAGAAATTGAATATGTACGTGAAAACTAC
  TATATAAATGAATCTGATGCTCAAAGTGTTCGTCAATTTGAAAATGAAATTCAAAGTTTA
  ATTTCTGTATATGATGATATTTTAAAAGAAATGTCTAAATCTGCTGTACGATATAGCGAG
  GTTCAGGATAATTTACAATATTTAGAAGATCATGTCACAGTTATTAATGACAAACAAGAA
  AAGCTACAAAATCATCTGATTCAATTGCGTGAAGATGAAGCAGAAGCAGAAGACAATCTG
  CTACGAGTACAATCGAAGAAAGAAGAAGTGTATCGTCGATTACTTGCTTCTAACTTAACA
  AGCGTTCCTGAAAGGTTTATCATCATGAAAAATGAAATTGATCATGAAGTTCGTGATGTT
  AACGAACAATTTAGTGAACGTCCAATACACGTTAAACAGTTAAAAGATAAAGTGTCTAAA
  ATTGTGATTCAAATGAATACATTTGAAGATGAAGCAAATGATGTTCTTGTTAATGCTGTT
  TATGCAGAGAAATTAATTCAATATGGAAATAGATATCGTAAGGACTATAGCAATGTTGAT
  AAGAGCTTAAATGAAGCTGAACGATTATTTAAAAATAATCGCTATAAGCGTGCGATTGAA
  ATTGCAGAGCAAGTTCTTGAAAGTGTTGAGCCAGGTGTCACTAAACATATTGAAGAAGAA
  GTTATTAAGCAATAG
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⊟Protein

⊟General

• locus tag: SA_RS08665 [old locus tag: SA1539 ]
• symbol: SA_RS08665
• description: septation ring formation regulator EzrA
• length: 564
• theoretical pI: 4.50272
• theoretical MW: 66227.1
• GRAVY: -0.682092

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 23.2)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 23.2)
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 22.7)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 22.7)
  and 2 more
  Genetic information processing Protein fate Protein folding and stabilization cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 5.9)
  Metabolism Energy metabolism Electron transport cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 5.9)
• TheSEED: see SA1539
• PFAM:
  no clan defined EzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 527.7)
  and 9 more
  PI3K_P85_iSH2; Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain (PF16454; HMM-score: 22.7)
  GPW_gp25; Gene 25-like lysozyme (PF04965; HMM-score: 18.1)
  Ac110_PIF; Per os infectivity factor AC110 (PF07280; HMM-score: 15.6)
  ASFV_J13L; African swine fever virus J13L protein (PF05568; HMM-score: 12.4)
  DUF2106; Predicted membrane protein (DUF2106) (PF09879; HMM-score: 12)
  Med9; RNA polymerase II transcription mediator complex subunit 9 (PF07544; HMM-score: 10.8)
  IncA; IncA protein (PF04156; HMM-score: 9.8)
  SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 9.2)
  HTH (CL0123) MerR-DNA-bind; MerR, DNA binding (PF09278; HMM-score: 7.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.94
  • Cytoplasmic Membrane Score: 2.06
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helix: 1
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.026729
  • TAT(Tat/SPI): 0.000705
  • LIPO(Sec/SPII): 0.007408
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446167011 NCBI
• RefSeq: WP_000244866 NCBI
• UniProt: see SA1539

⊟Protein sequence

• MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETLPFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNASQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANRHQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIPELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLELEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIEYVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHVTVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMKNEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQYGNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQVLESVEPGVTKHIEEEVIKQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications