PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0300
pan locus tag^?: SAUPAN001235000
symbol: SA0300
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0300
• symbol: SA0300
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 355764..356006
• length: 243
• essential: no DEG

⊟Accession numbers

• Gene ID: 1123079 NCBI
• RefSeq: NP_373546 NCBI
• BioCyc:
• MicrobesOnline: 102572 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  
  ATCAAGTCAGTTATGCCATCAAATAGTGTGAAAGATGTTACAGGTGCAGGCGATTCATTC
  TGTGCTGCAGTAGTATATAGCTGGTTAAATGGGATGTCTACTGAAGATATATTAATTGCT
  GGTATGGTTAACGCAAAGAAAACGATAGAAACGAAATATACAGTTAGGCAAAACCTAGAT
  CAACAGCAACTTTATCACGATATGGAGGATTATAAAAATGGCAAATTTACAAAAGTATAT
  TGA
  60
  120
  180
  240
  243
  

⊟Protein

⊟General

• locus tag: SA0300
• symbol: SA0300
• description: hypothetical protein
• length: 80
• theoretical pI: 7.34389
• theoretical MW: 9005.21
• GRAVY: -0.4125

⊟Function

• ⊞TIGRFAM:
  hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 25.5)
  1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 23)
  Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 21.6)
  and 2 more
  Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 16.9)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 16.2)
• TheSEED  :
  • Pseudouridine kinase (EC 2.7.1.83)
  Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Pseudouridine Metabolism  Pseudouridine kinase (EC 2.7.1.83)
• ⊞PFAM:
  Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 38.8)
  and 1 more
  AB_hydrolase (CL0028) Abhydrolase_10; Alpha/beta hydrolase domain (PF20091; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.4808
  • Cytoplasmic Membrane Score: 0.3086
  • Cell wall & surface Score: 0.0015
  • Extracellular Score: 0.2091
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.017008
  • TAT(Tat/SPI): 0.001803
  • LIPO(Sec/SPII): 0.00192
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926013 NCBI
• RefSeq: NP_373546 NCBI
• UniProt: A0A0H3JLB9 UniProt

⊟Protein sequence

• MKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGMVNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0300 > SA0301 > SA0302

⊟Regulation

• ⊞regulator: CcpA regulon

  CcpA

  (TF)

  important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications