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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2016 [new locus tag: SACOL_RS10530 ]
  • pan locus tag?: SAUPAN005241000
  • symbol: groEL
  • pan gene symbol?: groEL
  • synonym:
  • product: chaperonin GroEL

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2016 [new locus tag: SACOL_RS10530 ]
  • symbol: groEL
  • product: chaperonin GroEL
  • replicon: chromosome
  • strand: -
  • coordinates: 2076024..2077640
  • length: 1617
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGTTAAACAATTGAAATTCTCTGAAGATGCACGTCAAGCAATGTTACGTGGTGTTGAC
    CAACTTGCAAATGCAGTTAAAGTAACGATTGGTCCTAAAGGACGTAATGTTGTATTAGAT
    AAAGAGTTTACAGCACCTTTAATTACGAATGATGGTGTGACGATTGCTAAAGAAATCGAA
    TTAGAAGATCCATATGAAAATATGGGGGCTAAACTAGTTCAAGAAGTCGCAAATAAGACA
    AATGAAATTGCTGGTGACGGTACGACAACTGCAACAGTATTAGCTCAAGCAATGATTCAA
    GAAGGCTTGAAAAATGTTACAAGTGGTGCGAACCCAGTTGGTTTACGACAAGGTATCGAC
    AAAGCAGTTAAAGTTGCTGTTGAAGCGTTACATGAAAATTCTCAAAAAGTTGAAAATAAA
    AATGAAATTGCGCAAGTAGGTGCGATTTCAGCAGCAGATGAAGAAATTGGACGTTATATT
    TCTGAAGCTATGGAAAAAGTAGGTAACGATGGTGTCATTACAATTGAAGAATCAAATGGA
    CTAAACACTGAACTAGAAGTGGTTGAAGGTATGCAATTTGATCGTGGTTATCAATCACCG
    TATATGGTTACTGATTCAGATAAAATGGTTGCTGAATTAGAACGCCCATACATTTTAGTA
    ACAGATAAGAAAATCTCGTCTTTCCAAGATATCTTACCTTTATTAGAACAAGTGGTTCAA
    TCTAATCGTCCAATCTTAATTGTAGCTGATGAAGTTGAAGGCGATGCATTAACAAATATC
    GTGCTAAACCGTATGCGTGGCACATTTACAGCTGTTGCAGTAAAAGCACCTGGTTTTGGT
    GATCGTAGAAAAGCGATGCTTGAAGATTTAGCTATTTTAACTGGTGCGCAAGTGATTACT
    GATGATTTAGGCTTAGATTTAAAAGATGCATCAATTGATATGTTAGGTACTGCAAGTAAA
    GTAGAAGTAACTAAAGATAATACCACTGTTGTTGATGGTGACGGTGACGAAAACAGCATT
    GATGCACGTGTTAGCCAATTGAAATCTCAAATTGAAGAAACTGAATCTGACTTTGATCGT
    GAAAAATTACAAGAGCGCTTAGCTAAATTAGCAGGTGGTGTTGCAGTTATCAAAGTAGGT
    GCAGCAAGTGAAACAGAGCTTAAAGAACGTAAATTACGTATTGAAGATGCATTAAATTCT
    ACACGTGCAGCAGTTGAAGAAGGTATTGTTGCAGGTGGTGGTACTGCATTAGTAAATGTT
    TACCAAAAAGTAAGTGAAATTGAAGCTGAAGGTGACATTGAAACAGGTGTAAATATTGTA
    CTTAAAGCATTAACTGCACCAGTTCGTCAAATTGCTGAAAATGCAGGATTAGAAGGTTCT
    GTTATTGTAGAACGTTTGAAAAACGCAGAGCCGGGTGTTGGTTTTAACGCTGCTACAAAC
    GAGTGGGTTAATATGTTAGAAGAAGGTATCGTTGATCCAACTAAAGTAACACGCTCAGCA
    TTACAACATGCTGCAAGTGTTGCAGCAATGTTCTTAACGACTGAAGCGGTTGTAGCATCA
    ATTCCAGAAAAAAATAATGACCAACCTAACATGGGTGGCATGCCAGGAATGATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1617

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2016 [new locus tag: SACOL_RS10530 ]
  • symbol: GroEL
  • description: chaperonin GroEL
  • length: 538
  • theoretical pI: 4.27242
  • theoretical MW: 57629.9
  • GRAVY: -0.176952

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization chaperonin GroL (TIGR02348; HMM-score: 823.7)
    and 10 more
    Genetic information processing Protein fate Protein folding and stabilization thermosome, various subunits, archaeal (TIGR02339; HMM-score: 100.6)
    T-complex protein 1, delta subunit (TIGR02342; HMM-score: 92.7)
    T-complex protein 1, eta subunit (TIGR02345; HMM-score: 79.1)
    T-complex protein 1, epsilon subunit (TIGR02343; HMM-score: 75.1)
    T-complex protein 1, zeta subunit (TIGR02347; HMM-score: 72.1)
    T-complex protein 1, beta subunit (TIGR02341; HMM-score: 70.6)
    T-complex protein 1, gamma subunit (TIGR02344; HMM-score: 63.8)
    T-complex protein 1, theta subunit (TIGR02346; HMM-score: 60.3)
    T-complex protein 1, alpha subunit (TIGR02340; HMM-score: 50.1)
    L-seryl-tRNA(Sec) kinase (TIGR03575; EC 2.7.1.164; HMM-score: 15)
  • ⊞TheSEED  :
    • Heat shock protein 60 family chaperone GroEL
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Heat shock protein 60 family chaperone GroEL
    and 1 more
    Protein Metabolism Protein folding GroEL GroES  Heat shock protein 60 family chaperone GroEL
  • PFAM:
    no clan defined Cpn60_TCP1; TCP-1/cpn60 chaperonin family (PF00118; HMM-score: 260.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9849
    • Cytoplasmic Membrane Score: 0.0017
    • Cell wall & surface Score: 0.0009
    • Extracellular Score: 0.0125
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004121
    • TAT(Tat/SPI): 0.001123
    • LIPO(Sec/SPII): 0.000853
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFTAPLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYISEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYILVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKAPGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVAAMFLTTEAVVASIPEKNNDQPNMGGMPGMM

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 4689 [5]
  • ⊟interaction partners:
    SACOL0594(tuf)elongation factor Tu  [6] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: HrcA (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 31.05 h [7]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  7. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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