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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0260 [new locus tag: NWMN_RS01435 ]
  • pan locus tag?: SAUPAN001242000
  • symbol: nanE
  • pan gene symbol?: nanE
  • synonym:
  • product: N-acetylmannosamine-6-phosphate 2-epimerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0260 [new locus tag: NWMN_RS01435 ]
  • symbol: nanE
  • product: N-acetylmannosamine-6-phosphate 2-epimerase
  • replicon: chromosome
  • strand: +
  • coordinates: 316295..316963
  • length: 669
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTTACCACATGGATTAATAGTATCTTGTCAGGCACTACCAGATGAACCATTGCATTCA
    TCTTTTATTATGTCGAAAATGGCATTAGCTGCGTATGAAGGTGGTGCTGTTGGTATTCGC
    GCAAATACTAAGGAAGACATTTTAGCAATTAAAGAAACGGTAGATTTACCAGTTATTGGC
    ATTGTGAAACGTGACTATGATCACTCAGATGTTTTCATTACTGCAACGTCAAAAGAAGTT
    GATGAACTGATAGAAAGCCAATGTGAAGTCATTGCATTGGATGCAACGTTACAGCAACGT
    CCGAAAGAAACGTTAGACGAATTAGTATCATATATTAGAACACATGCACCGAACGTTGAA
    ATCATGGCTGATATCGCGACCGTTGAAGAAGCTAAAAATGCCGCACGACTTGGCTTTGAT
    TATATTGGCACGACGTTACATGGCTATACTAGTTATACGCAAGGACAATTACTTTATCAA
    AATGACTTCCAATTTTTAAAAGATGTACTACAAAGTGTTGATGCAAAAGTTATTGCGGAA
    GGTAATGTCATTACACCGGATATGTATAAACGTGTGATGGACTTAGGCGTTCATTGTTCA
    GTCGTTGGTGGTGCGATAACACGACCAAAAGAAATTACGAAACGTTTTGTTCAAATTATG
    GAAGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    669

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0260 [new locus tag: NWMN_RS01435 ]
  • symbol: NanE
  • description: N-acetylmannosamine-6-phosphate 2-epimerase
  • length: 222
  • theoretical pI: 4.57542
  • theoretical MW: 24545
  • GRAVY: -0.0189189

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 5.1.3.9?  ExPASy
    N-acylglucosamine-6-phosphate 2-epimerase N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate
  • ⊞TIGRFAM:
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 28.3)
    and 3 more
    Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 14.2)
    bifunctional HisA/TrpF protein (TIGR01919; EC 5.3.1.16,5.3.1.24; HMM-score: 13.3)
    Cellular processes Cellular processes Toxin production and resistance radical SAM P-methyltransferase, PhpK family (TIGR04479; EC 2.1.-.-; HMM-score: 12.9)
  • TheSEED  :
    • N-acetylmannosamine-6-phosphate 2-epimerase (EC 5.1.3.9)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylmannosamine-6-phosphate 2-epimerase (EC 5.1.3.9)
  • ⊞PFAM:
    TIM_barrel (CL0036) NanE; Putative N-acetylmannosamine-6-phosphate epimerase (PF04131; HMM-score: 217)
    and 3 more
    IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 20.5)
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 15.4)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9848
    • Cytoplasmic Membrane Score: 0.0119
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0031
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.023929
    • TAT(Tat/SPI): 0.011302
    • LIPO(Sec/SPII): 0.002163
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTKEDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPKETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQNDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQIMED

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: NanR* (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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