⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS04295 [old locus tag: SA0753 ]
pan locus tag^?: SAUPAN002812000
symbol: SA_RS04295
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS04295 [old locus tag: SA0753 ]
• symbol: SA_RS04295
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 861025..861642
• length: 618
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (861025..861642) NCBI
• BioCyc: SA_RS04295 BioCyc
• MicrobesOnline: see SA0753

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGGTAACCGCAATTATTCATGGATTTATCTTAGCGATTGGTCTAATTTTACCTTTAGGT
  GCACAAAATGTATTTATTTTTAACCAAGGAGCTAATCAACCAAAATATAGATATGTATTG
  CCTGCAATAATTACAGCCGGGTTGTCAGACAGCTTACTTATTATTATTGCAGTGGTAGGA
  GTATCTATCATTATTATGTCTTTACCTGTACTTCAAGCAATTATTTATATAGTTGGTTTA
  ATTTTCTTGATGTATATGGCTTGGACCATTTGGCATGATAAACCCTCAACAGATGGAGAA
  GCTCAAATTATATCTCCAATGAAACAAGTAAGTTTTGCTTTATCAGTTTCATTACTCAAT
  CCACATGCTATTTTAGATACAATTGGTGTAATTGGTAGTAGTGCTGCATTATATAGTGGC
  AGCAATAAAATTGCGTTTACAATCGCTTGTATTAGCGTTTCATGGTTATGGTTCTTTTTA
  CTCGCAATTTTAGGAAAAATGGTTGGATCAATTGATAAAACTGGAAAATTATTAACAATA
  ATCAATAAAATATCAAGCATAATAATTATTATTGTTGCTCTAATGATATTACAAAAACTT
  ATTCAATTATTATTCTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  618
  

⊟Protein

⊟General

• locus tag: SA_RS04295 [old locus tag: SA0753 ]
• symbol: SA_RS04295
• description: hypothetical protein
• length: 205
• theoretical pI: 9.71156
• theoretical MW: 22163.8
• GRAVY: 1.20976

⊟Function

• ⊞⊞TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines L-lysine exporter (TIGR00948; HMM-score: 152.2)
  and 1 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines homoserine/Threonine efflux protein (TIGR00949; HMM-score: 35)
• TheSEED: see SA0753
• ⊞⊞PFAM:
  LysE (CL0292) LysE; LysE type translocator (PF01810; HMM-score: 93.7)
  and 1 more
  no clan defined CAAD; CAAD domains of cyanobacterial aminoacyl-tRNA synthetase (PF14159; HMM-score: 14.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• ⊞⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9999
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0001
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.038834
  • TAT(Tat/SPI): 0.001598
  • LIPO(Sec/SPII): 0.07802
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 446177696 NCBI
• RefSeq: WP_000255551 NCBI
• UniProt: see SA0753

⊟Protein sequence

• MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAIITAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQIISPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFLLAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SA0753

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications