From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0870 [new locus tag: SACOL_RS04465 ]
  • pan locus tag?: SAUPAN002812000
  • symbol: SACOL0870
  • pan gene symbol?: —
  • synonym:
  • product: LysE/YggA family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0870 [new locus tag: SACOL_RS04465 ]
  • symbol: SACOL0870
  • product: LysE/YggA family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 892991..893608
  • length: 618
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTAACCGCAATTATTCATGGATTTATCTTAGCGATTGGTCTAATTTTACCTTTAGGT
    GCACAAAATGTATTTATTTTTAACCAAGGAGCTAATCAACCAAAATATAGATATGTATTG
    CCTGCAATAATTACAGCCGGGTTGTCAGACAGCTTACTTATTATTATTGCAGTGGTAGGA
    GTATCTATCATTATTATGTCTTTACCTGTACTTCAAGCAATTATTTATATAGTTGGTTTA
    ATTTTCTTGATGTATATGGCTTGGACCATTTGGCATGATAAACCCTCAACAGATGGAGAA
    ACTCAAATTATGTCTCCAATGAAACAAGTAAGTTTTGCTTTATCAGTTTCATTACTCAAT
    CCACATGCTATTTTAGATACAATTGGAGTAATTGGTAGTAGTGCTGCATTATATAGTGGC
    AGCAATAAAATTGCGTTTACAATCGCTTGTATTAGCGTTTCATGGTTATGGTTCTTTTTA
    CTCGCAATTTTAGGAAAAATGGTTGGATCAATTGATAAAACCGGAAAATTATTAACAATA
    ATCAATAAAATATCAAGCATAATAATTATTATTGTTGCTCTGATGATATTACAAAAACTT
    ATTCAATTATTATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    618

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0870 [new locus tag: SACOL_RS04465 ]
  • symbol: SACOL0870
  • description: LysE/YggA family protein
  • length: 205
  • theoretical pI: 9.71156
  • theoretical MW: 22211.9
  • GRAVY: 1.18488

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-lysine exporter (TIGR00948; HMM-score: 154.3)
    and 1 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines homoserine/Threonine efflux protein (TIGR00949; HMM-score: 35.3)
  • TheSEED  :
    • Transporter, LysE family
  • PFAM:
    LysE (CL0292) LysE; LysE type translocator (PF01810; HMM-score: 94.9)
    and 1 more
    no clan defined CAAD; CAAD domains of cyanobacterial aminoacyl-tRNA synthetase (PF14159; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.038834
    • TAT(Tat/SPI): 0.001598
    • LIPO(Sec/SPII): 0.07802
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAIITAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQIMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFLLAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL0870&oldid=54316"