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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0635 [new locus tag: SACOL_RS03280 ]
  • pan locus tag?: SAUPAN002369000
  • symbol: SACOL0635
  • pan gene symbol?: lipL
  • synonym:
  • product: lipoate-protein ligase A family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0635 [new locus tag: SACOL_RS03280 ]
  • symbol: SACOL0635
  • product: lipoate-protein ligase A family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 666736..667572
  • length: 837
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGATTTAGCGAGTAAATATTTTAATGGCGTCAACTGGCGATATATCGATCATTCTTCT
    GGATTAGAACCTATGCAATCTTTCGCATTCGATGATACATTTTGCGAAAGTGTGGGTAAA
    GATATATCAGATAATGTTGTGCGTACTTGGATTCATCAACATACTGTTATTCTTGGTATT
    CATGATTCAAGATTGCCGTTTTTAAAAGATGGCATTGATTATTTAACGAATGAGATTGGT
    TATAATGCCATTGTTAGAAATTCTGGTGGCTTAGGTGTCGTTCTAGATCAAGGTGTATTA
    AATATATCGCTGATGTTCAAAGGACAAACAGAAACAACGATTGATGAAGCGTTTACTGTG
    ATGTACCTCTTAATTAGCAAAATGTTCGAAAATGAGAATGTTGATATTGATACGATGGAA
    ATTGAACATTCTTATTGCCCAGGAAAATTTGACTTAAGTATCGATGGTAAGAAATTTGCA
    GGCATATCGCAACGAAGAGTTAGAGGCGGTATTGCTGTACAAATTTATCTTTGTGTTGAA
    GGCTCTGGTTCAGAACGTGCATTGATGATGCAAACATTTTATGAACATGCTTTAAAAGGT
    GAAGTGACTAAATTTAAATATCCTGAAATTGAACCATCTTGTATGGCCTCATTAGAGACA
    TTGCTTAACAAAACGATTACTGTTCAAGATGTAATGTTTTTACTATTATATGCAATCAAA
    GATCTTGGCGGTGTATTAAATATGACGCCAATTACTCAAGAAGAATGGCAAAGATACGAT
    ACGTATTTTGATAAAATGATTGAAAGAAACAAGAAAATGATAGATCAAATGCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    837

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0635 [new locus tag: SACOL_RS03280 ]
  • symbol: SACOL0635
  • description: lipoate-protein ligase A family protein
  • length: 278
  • theoretical pI: 4.66423
  • theoretical MW: 31735.2
  • GRAVY: -0.161151

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.3.1.204?  ExPASy
    Octanoyl-[GcvH]:protein N-octanoyltransferase [Glycine cleavage system H]-N6-octanoyl-L-lysine + a [lipoyl-carrier protein] = glycine cleavage system H + a [lipoyl-carrier protein]-N6-octanoyl-L-lysine
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair lipoyltransferase and lipoate-protein ligase (TIGR00545; HMM-score: 18.3)
  • ⊞TheSEED  :
    • Protein:protein lipoyl transferase
    Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Lipoate-protein ligase A
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Lipoic acid Lipoic acid metabolism  Lipoate-protein ligase A
  • ⊞PFAM:
    tRNA_synt_II (CL0040) LplA-B_cat; Lipoyl protein ligase A/B catalytic domain (PF21948; HMM-score: 122.4)
    and 1 more
    BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 46.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9873
    • Cytoplasmic Membrane Score: 0.0099
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0026
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003675
    • TAT(Tat/SPI): 0.000136
    • LIPO(Sec/SPII): 0.000416
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISDNVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNISLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFAGISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASLETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQMQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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